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07-2162 Anti-PCNA Antibody

Use Anti-PCNA Antibody (Rabbit Polyclonal Antibody) validated in WB, ICC to detect PCNA also known as proliferating cell nuclear antigen, cyclin, DNA polymerase delta auxiliary protein, Cyclin.

Anti-PCNA Antibody MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Epigenetics & Nuclear Function Research Sub-Category: Cell Cycle, DNA Replication & Repair Gene Symbol: PCNA UniProt Number: P12004
07-2162
100 µg  
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Tabulka spec. kláve

Species ReactivityKey ApplicationsHostFormatAntibody Type
H, M, R, ChpWB, ICCRbPurifiedPolyclonal Antibody
Description
Catalogue Number07-2162
DescriptionAnti-PCNA Antibody
Alternate Names
  • proliferating cell nuclear antigen
  • cyclin
  • DNA polymerase delta auxiliary protein
  • Cyclin
Background InformationProliferating cell nuclear antigen (PCNA) belongs to the DNA sliding clamp family and is comprised of three identical monomers forming a heterodimer. Due to its unique structure, PCNA is able to increase the processivity of DNA polymerase delta by using its outer domains to be involved in protein-protein interaction while still tethered to and freely sliding along the DNA. PCNA is a critical regulatory component in the onset of leading strand DNA replication and the termination of lagging strand synthesis. Studies have also indicated a role for PCNA in the recruitment and regulation of MMR pathway repair proteins. PCNA is also able to interact with a wide variety of cell cycle proteins and serves to coordinate several proteins participating in DNA processes, such as apoptosis.
References
Product Information
FormatPurified
Control
  • A431 cell lysate
PresentationPurified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationUse Anti-PCNA Antibody (Rabbit Polyclonal Antibody) validated in WB, ICC to detect PCNA also known as proliferating cell nuclear antigen, cyclin, DNA polymerase delta auxiliary protein, Cyclin.
Key Applications
  • Western Blotting
  • Immunocytochemistry
Application NotesWestern Blot Analysis: 1 µg/mL from a previous lot detected PCNA on 10 µg of NIH/3T3 and PC12 cell lysates.

Immunocytochemistry Analysis: 1:500 dilution from a previous lot detected PCNA in A431 cells.
Biological Information
ImmunogenGST-tagged recombinant protein corresponding to human PCNA.
ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
HostRabbit
Species Reactivity
  • Human
  • Mouse
  • Rat
  • Chimpanzee
Species Reactivity NoteDemonstrated to react with human, mouse, and rat. Predicted to react with chimpanzee based on 100% sequence homology. Other homologies: Bovine, rabbit, canine, and panda (99% sequence homology).
Antibody TypePolyclonal Antibody
Entrez Gene Number
Entrez Gene SummaryThe protein encoded by this gene is found in the nucleus and is a cofactor of DNA polymerase delta. The encoded protein acts as a homotrimer and helps increase the processivity of leading strand synthesis during DNA replication. In response to DNA damage, this protein is ubiquitinated and is involved in the RAD6-dependent DNA repair pathway. Two transcript variants encoding the same protein have been found for this gene. Pseudogenes of this gene have been described on chromosome 4 and on the X chromosome. [provided by RefSeq].
Gene Symbol
  • PCNA
Purification MethodProtein A
UniProt Number
UniProt SummaryFUNCTION: This protein is an auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand.

SUBUNIT STRUCTURE: Homotrimer (By similarity). Forms a complex with activator 1 heteropentamer in the presence of ATP. Interacts with EXO1, POLH, POLK, DNMT1, ERCC5, FEN1, CDC6, APEX2 and POLDIP2. Forms a ternary complex with DNTTIP2 and core histone. Interacts with KCTD10 and PPP1R15A (By similarity). Interacts with POLD1, POLD3 and POLD4. Interacts with BAZ1B; the interaction is direct. Interacts with HLTF and SHPRH. Interacts with NUDT15. Interaction is disrupted in response to UV irradiation and acetylation. Interacts with CDKN1A/p21(CIP1) and CDT1; interacts via their PIP-box which also recruits the DCX(DTL) complex.

SUBCELLULAR LOCATION: Nucleus.

PTM: Upon methyl methanesulfonate-induced DNA damage, mono-ubiquitinated by the UBE2B-RAD18 complex on Lys-164. This induces non-canonical polyubiquitination on Lys-164 through 'Lys-63' linkage of ubiquitin moieties by the E2 complex UBE2N-UBE2V2 and the E3 ligases, HLTF, RNF8 and SHPRH, which is required for DNA repair. 'Lys-63' polyubiquitination prevents genomic instability on DNA damage. Monoubiquitination at Lys-164 also takes place in undamaged proliferating cells, and is mediated by the DCX(DTL) complex, leading to enhance PCNA-dependent translesion DNA synthesis.

Acetylated in response to UV irradiation. Acetylation disrupts interaction with NUDT15 and promotes degradation.

MISCELLANEOUS: Antibodies against PCNA are present in sera from patients with systemic lupus erythematosus.

SEQUENCE SIMILARITIES: Belongs to the PCNA family.
Molecular Weight~ 35 kDa observed. PCNA has a predicted molecular weight of 29 kDa, however, it can be monoubiquitinated, resulting in a ~ 35 kDa band.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blot in A431 cell lysate.

Western Blot Analysis: 1 µg/mL of this antibody detected PCNA on 10 µg of A431 cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 µg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Katalogové číslo GTIN
07-2162 04053252641909

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Millipore’s antibodies against Proliferating cell nuclear antigen (PCNA) have been well validated and published. A list of our PCNA antibodies, based on the expertise of Upstate & Chemicon, is below.
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Kategorie

Life Science Research > Antibodies and Assays > Primary Antibodies