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NA03T Anti-PCNA (112-121) (Ab-1) Mouse mAb (PC10)

Anti-PCNA (112-121) (Ab-1) Mouse mAb (PC10) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Anti-Proliferating Cell Nuclear Antigen

NA03T
  
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Přehled

Replacement Information

Tabulka spec. kláve

Host
M
Description
OverviewRecognizes the ~37 kDa PCNA protein in HeLa cells and in tonsil and normal colon tissue.
Catalogue NumberNA03T
Brand Family Calbiochem®
SynonymsAnti-Proliferating Cell Nuclear Antigen
References
ReferencesWaseem, N.H. and Lane, D.P. 1990. J. Cell. Sci. 96, 121. Suzuka, I., et al. 1989. Proc. Natl. Acad. Sci. USA 86 3189. Bravo, R., et al. 1987. Nature (London) 326, 515. Bravo, R. and MacDonald-Bravo, H. 1987. J. Cell. Biol. 105, 1549. Miyachi, K., et al. 1987. J. Immunol. 121, 2228.
Product Information
FormLiquid
FormulationIn 50 mM sodium phosphate buffer, 50% glycerol.
Positive controlHeLa cells, HL-60 cells, tonsil tissue, or normal colon tissue
PreservativeNone
Applications
Key Applications Immunoblotting (Western Blotting)
Immunofluorescence
Not Immunoprecipitation
Paraffin Sections
Application NotesImmunoblotting (2.5 µg/ml) Immunofluorescence (1-5 µg/ml) Immunoprecipitation (not recommended with unconjugated antibody, see comments) Paraffin Sections (2.5 µg/ml, no pre-treatment required, see comments)
Application CommentsStaining shows an intense nuclear pattern. Not recommended for immunoprecipitation because a ~37 kDa protein will be precipitated that is not PCNA in some samples. Generally, no pre-treatment is needed to stain paraffin sections, but staining may be enhanced in some samples by pre-treating with 4 N HCl for 10 min at 37°C followed by 3 washes in water. Antibody should be titrated for optimal results in individual systems.
Biological Information
Immunogenrecombinant PCNA
Epitopewithin amino acids 112-121
ClonePC10
HostMouse
IsotypeIgG2a
Concentration Label Please refer to vial label for lot-specific concentration
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Dry Ice Only
Toxicity Standard Handling
Storage -20°C
Protect from Moisture Protect from moisture
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Katalogové číslo GTIN
NA03T 0

Documentation

Anti-PCNA (112-121) (Ab-1) Mouse mAb (PC10) Certificates of Analysis

TitleLot Number
NA03T

References

Přehled odkazů
Waseem, N.H. and Lane, D.P. 1990. J. Cell. Sci. 96, 121. Suzuka, I., et al. 1989. Proc. Natl. Acad. Sci. USA 86 3189. Bravo, R., et al. 1987. Nature (London) 326, 515. Bravo, R. and MacDonald-Bravo, H. 1987. J. Cell. Biol. 105, 1549. Miyachi, K., et al. 1987. J. Immunol. 121, 2228.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision04-September-2007 RFH
SynonymsAnti-Proliferating Cell Nuclear Antigen
ApplicationImmunoblotting (2.5 µg/ml) Immunofluorescence (1-5 µg/ml) Immunoprecipitation (not recommended with unconjugated antibody, see comments) Paraffin Sections (2.5 µg/ml, no pre-treatment required, see comments)
DescriptionPurified mouse monoclonal antibody generated by immunizing BALB/c mice with the specified immunogen and fusing splenocytes with myeloma, SP2/0-Ag14. Recognizes the ~37 kDa PCNA protein.
BackgroundThe proliferating cell nuclear antigen (PCNA) is a ~37 kDa molecular weight protein also known as cyclin. The protein has also been identified as the polymerase δ accessory protein and is detected in a cell cycle dependent manner. In early S phase, PCNA has a very granular distribution and is absent from the nucleoli. At late S phase, PCNA is prominent in the nucleoli. In cells fixed with organic solvents, the PCNA is seen to be strongly associated in the nuclear regions where DNA synthesis is occurring, whereas in cells fixed with aldehydes, the staining is more diffuse but intense and occurs throughout the cell cycle. This is due to the presence of two basic forms of the PCNA protein: a soluble form which is sensitive to organic fixation and not involved in replication and a second form which is insoluble and associated with on-going DNA synthesis. PCNA is a very conserved protein present not only in mammals but also in plant cells. Patients suffering from SLE have been shown to have auto antibodies against PCNA.
HostMouse
Immunogenrecombinant PCNA
Epitopewithin amino acids 112-121
ClonePC10
IsotypeIgG2a
Specieshuman, mouse, rat, yeast
Positive controlHeLa cells, HL-60 cells, tonsil tissue, or normal colon tissue
FormLiquid
FormulationIn 50 mM sodium phosphate buffer, 50% glycerol.
Concentration Label Please refer to vial label for lot-specific concentration
PreservativeNone
CommentsStaining shows an intense nuclear pattern. Not recommended for immunoprecipitation because a ~37 kDa protein will be precipitated that is not PCNA in some samples. Generally, no pre-treatment is needed to stain paraffin sections, but staining may be enhanced in some samples by pre-treating with 4 N HCl for 10 min at 37°C followed by 3 washes in water. Antibody should be titrated for optimal results in individual systems.
Storage Protect from moisture
Avoid freeze/thaw
-20°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Toxicity Standard Handling
ReferencesWaseem, N.H. and Lane, D.P. 1990. J. Cell. Sci. 96, 121. Suzuka, I., et al. 1989. Proc. Natl. Acad. Sci. USA 86 3189. Bravo, R., et al. 1987. Nature (London) 326, 515. Bravo, R. and MacDonald-Bravo, H. 1987. J. Cell. Biol. 105, 1549. Miyachi, K., et al. 1987. J. Immunol. 121, 2228.