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06-1330 Anti-PBX1 Antibody

Anti-PBX1 Antibody is a rabbit polyclonal antibody for detection of PBX1 also known as pre-B-cell leukemia homeobox 1, pre-B-cell leukemia transcription factor 1, Homeobox protein PRL & has been validated in WB, ICC.

Anti-PBX1 Antibody MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Epigenetics & Nuclear Function, Stem Cell Research Research Sub-Category: Transcription Factors, Transcription Factors Gene Symbol: PBX1, PRL UniProt Number: P40424
06-1330
100 µL  
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Tabulka spec. kláve

Species ReactivityKey ApplicationsHostFormatAntibody Type
H, Chp, OpWB, ICCRbAffinity PurifiedPolyclonal Antibody
Description
Catalogue Number06-1330
DescriptionAnti-PBX1 Antibody
Alternate Names
  • pre-B-cell leukemia homeobox 1
  • pre-B-cell leukemia transcription factor 1
  • Homeobox protein PRL
  • Pre-B cell leukemia transcription factor-1
  • Homeobox protein PBX1
Background InformationPre-B-cell leukemia transcription factor 1 (PBX1) is a TALE-class homeodomain transcription factor belonging to the TALE/PBX homeobox family. PBX1 functions as a transcriptional activator of PF4 in complex with MEIS1, facilitating the formation of trimeric transcriptional complexes with Hox proteins. Expression has been observed in human fetal brain which may play a role in the generation of the olfactory bulb interneurons and in mammalian neurogenesis. Mutations in PBX1 have been associated with pre-B-cell acute lymphoblastic leukemia (B-ALL).
References
Product Information
FormatAffinity Purified
Control
  • HeLa nuclear extract
PresentationPurified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Applications
ApplicationAnti-PBX1 Antibody is a rabbit polyclonal antibody for detection of PBX1 also known as pre-B-cell leukemia homeobox 1, pre-B-cell leukemia transcription factor 1, Homeobox protein PRL & has been validated in WB, ICC.
Key Applications
  • Western Blotting
  • Immunocytochemistry
Application NotesWestern Blot (SNAP i.d.) Analysis: A 1:500 dilution from a representative lot detected PBX1 on 10 µg of HeLa nuclear extract.

Immunocytochemistry Analysis: 1:500 dilution from a representative lot detected PBX1 in NIH/3T3 and HeLa cells.
Biological Information
ImmunogenKLH-conjugated linear peptide corresponding to the N-terminus of human PBX1.
EpitopeN-terminus
ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
HostRabbit
SpecificityThis antibody recognizes PBX1 at the N-terminus.
Species Reactivity
  • Human
  • Chimpanzee
  • Opossum
Species Reactivity NoteDemonstrated to react with human. Predicted to react with chimpanzee, opossum, panda, orangutan, platypus, and canine based on 100% sequence homology.
Antibody TypePolyclonal Antibody
Entrez Gene Number
Gene Symbol
  • PBX1
  • PRL
Purification MethodAffinity Purfied
UniProt Number
UniProt SummaryFUNCTION: Binds the sequence 5'-ATCAATCAA-3'. Acts as a transcriptional activator of PF4 in complex with MEIS1. Converted into a potent transcriptional activator by the (1;19) translocation. May have a role in steroidogenesis and, subsequently, sexual development and differentiation. Ref.10

SUBUNIT STRUCTURE: Forms a heterodimer with MEIS1 which binds DNA including a cAMP-responsive sequence in CYP17. Also forms heterotrimers with MEIS1 and a number of HOX proteins including HOXA9, HOXD4, HOXD9 and HOXD10. Interacts with PBXIP1.

SUBCELLULAR LOCATION: Nucleus Probable.

TISSUE SPECIFICTY: Expressed in all tissues except in cells of the B and T lineage.

INVOLVEMENT IN DISEASE: Note=A chromosomal aberration involving PBX1 is a cause of pre-B-cell acute lymphoblastic leukemia (B-ALL). Translocation t(1;19)(q23;p13.3) with TCF3. E2A-PBX1 transforms cells by constitutively activating transcription of genes regulated by PBX1 or by other members of the PBX protein family.

SEQUENCE SIMILARITIES: Belongs to the TALE/PBX homeobox family.

Contains 1 homeobox DNA-binding domain.

SEQUENCE CAUTION: The sequence AAA36764.1 differs from that shown. Reason: Erroneous initiation.
Molecular Weight~39 and ~53 kDa observed. Due to alternative splicing PBX1 is observed with two isoforms, Pbx1a and 1b, that are divergent in sequence at the carboxy terminus.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blot in HeLa nuclear extract.

Western Blot Analysis: 1:2000 dilution of this antibody detected PBX1 on 10 µg of HeLa nuclear extract.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Katalogové číslo GTIN
06-1330 04053252748547