Millipore Sigma Vibrant Logo
Attention: We have moved. Merck Millipore products are no longer available for purchase on MerckMillipore.com.Learn More

PC362 Anti-Orexin A (14-33) (Ab-2) Rabbit pAb

Anti-Orexin A (14-33) (Ab-2) Rabbit pAb MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
PC362
  
Purchase on Sigma-Aldrich

Přehled

Replacement Information

Tabulka spec. kláve

Species ReactivityHostAntibody Type
B, H, M, RRbPolyclonal Antibody
Description
Overview

This product has been discontinued.



Recognizes orexin A in mouse and rat brain. Supplied with a control peptide.
Catalogue NumberPC362
Brand Family Calbiochem®
Application Data
Detection of human Orexin A by staining free-floating sections. Sample: Floating mouse brain fixed in 4% paraformaldehyde. Primary antibody: Anti-Orexin A (14-33) (Ab-2) Rabbit pAb (Cat. No. PC362) (1:3500).
References
ReferencesDe Lecea, L., et al. 1998. Proc. Natl. Acad. Sci. USA 95, 322.
Flier, J.S. and Maratos-Flier, E. 1998. Cell 92, 437.
Sakurai, T., et al. 1998. Cell 92, 573.
Schwartz, M.W. 1998. Nat. Med. 4, 385.
Product Information
FormLiquid
FormulationSerum or ascites diluted in 5 mM phosphate buffer, pH 7.5 and 25 µg lyophilized control.
Positive controlMouse or rat brain
Preservative≤0.1% sodium azide (antibody only)
Quality LevelMQ100
Applications
Key Applications Enzyme-Linked Immunosorbent Assay
Free-floating Sections
Frozen Sections
Immunofluorescence
Application NotesELISA (see comments)
Free-floating Sections (1:3500)
Frozen Sections (1:3500)
Immunofluorescence (1:2000)
Application CommentsImmunohistochemistry was performed using floating mouse and rat brain sections fixed in 4% paraformaldehyde. Staining was completely abolished by preincubating the antiserum with control peptide at 10-6M. Staining is observed in the lateral hyothalamus. Unconjugated Orexin A Control Peptide has been included for use as an internal control for antibody specificity. This antibody has been tested to work in ELISA. Also available in purified form, Anti-Orexin A (14-33) (Ab-1) Rabbit pAb, Cat. No. PC345. Antibody should be titrated for optimal results in individual systems.
Biological Information
Immunogena synthetic peptide (CRLYELLHGAGNHAAGILTL) corresponding to amino acids 14-33 of human orexin A
ImmunogenHuman
HostRabbit
IsotypeIgG
Species Reactivity
  • Bovine
  • Human
  • Mouse
  • Rat
Antibody TypePolyclonal Antibody
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Dry Ice Only
Toxicity Standard Handling
Storage -20°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsFollowing initial thaw of antibody, aliquot and freeze (-20°C). Reconstitute control peptide with 25 µl dH₂O for a working stock of 1 mg/ml. Following reconstitution, aliquot and freeze (-20°C).
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Katalogové číslo GTIN
PC362 0

Documentation

Anti-Orexin A (14-33) (Ab-2) Rabbit pAb MSDS

Title

Safety Data Sheet (SDS) 

Anti-Orexin A (14-33) (Ab-2) Rabbit pAb Certificates of Analysis

TitleLot Number
PC362

References

Přehled odkazů
De Lecea, L., et al. 1998. Proc. Natl. Acad. Sci. USA 95, 322.
Flier, J.S. and Maratos-Flier, E. 1998. Cell 92, 437.
Sakurai, T., et al. 1998. Cell 92, 573.
Schwartz, M.W. 1998. Nat. Med. 4, 385.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision09-October-2007 RFH
ApplicationELISA (see comments)
Free-floating Sections (1:3500)
Frozen Sections (1:3500)
Immunofluorescence (1:2000)
Application Data
Detection of human Orexin A by staining free-floating sections. Sample: Floating mouse brain fixed in 4% paraformaldehyde. Primary antibody: Anti-Orexin A (14-33) (Ab-2) Rabbit pAb (Cat. No. PC362) (1:3500).
DescriptionRabbit polyclonal antibody supplied as undiluted serum. Recognizes orexin A. Supplied with a control peptide.
BackgroundThe hypothalamus has been shown to play a central role in the regulation of energy homeostasis and in the control of feeding behavior. Two homologous peptides have recently been identified for which the message and protein have been localized to the lateral hypothalamus. Orexin A (hypocretin 1), a 33 amino acid peptide, and orexin B (hypocretin 2), a 28 amino acid peptide, are both encoded by a single mRNA transcript. These peptides stimulate food consumption in a dose-dependent manner when administered intracerebroventricularly, and precursor mRNA levels are upregulated upon fasting. In addition to the orexins (hypocretins), two G-protein coupled receptors, designated orexin receptor-1 and orexin receptor-2, have been identified which presumably mediate the signaling events from these neuropeptides. Competitive radioligand binding assays suggest that the orexin receptor-1 is selective for orexin A, while orexin receptor-2 is nonselective for both peptides.
HostRabbit
Immunogen speciesHuman
Immunogena synthetic peptide (CRLYELLHGAGNHAAGILTL) corresponding to amino acids 14-33 of human orexin A
IsotypeIgG
Speciesbovine, human, mouse, rat
Positive controlMouse or rat brain
FormLiquid
FormulationSerum or ascites diluted in 5 mM phosphate buffer, pH 7.5 and 25 µg lyophilized control.
Preservative≤0.1% sodium azide (antibody only)
CommentsImmunohistochemistry was performed using floating mouse and rat brain sections fixed in 4% paraformaldehyde. Staining was completely abolished by preincubating the antiserum with control peptide at 10-6M. Staining is observed in the lateral hyothalamus. Unconjugated Orexin A Control Peptide has been included for use as an internal control for antibody specificity. This antibody has been tested to work in ELISA. Also available in purified form, Anti-Orexin A (14-33) (Ab-1) Rabbit pAb, Cat. No. PC345. Antibody should be titrated for optimal results in individual systems.
Storage Avoid freeze/thaw
-20°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing initial thaw of antibody, aliquot and freeze (-20°C). Reconstitute control peptide with 25 µl dH₂O for a working stock of 1 mg/ml. Following reconstitution, aliquot and freeze (-20°C).
Toxicity Standard Handling
ReferencesDe Lecea, L., et al. 1998. Proc. Natl. Acad. Sci. USA 95, 322.
Flier, J.S. and Maratos-Flier, E. 1998. Cell 92, 437.
Sakurai, T., et al. 1998. Cell 92, 573.
Schwartz, M.W. 1998. Nat. Med. 4, 385.