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ABE2895-25UL Anti-ORF0

Anti-ORF0 Antibody, Cat. No. ABE2895, is a highly specific rabbit polyclonal antibody, that targets and has been tested in Immunoprecipitation, Immunocytochemistry and Western Blotting.

Anti-ORF0 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Epigenetics & Nuclear Function Gene Symbol: ORF0 UniProt Number: N/A
ABE2895-25UL
25 µL  
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Replacement Information

Tabulka spec. kláve

Species ReactivityKey ApplicationsHostFormatAntibody Type
PmIP, ICC, WBRbUnpurifiedPolyclonal Antibody
Description
Catalogue NumberABE2895-25UL
DescriptionAnti-ORF0
Alternate Names
  • ORF0 protein
  • Open Reading Frame 0
Background InformationOpen Reading Frame 0 (ORF0) is a primate-specific open reading frame in LINE-1 retrotransposons. Over 3,000 potential ORF0 loci are reported to be present in human and chimpanzee genomes. ORF0 is a predominantly nuclear protein that enhances LINE-1 mobility and contributes to retrotransposon-mediated diversity. It is a highly positively charged protein that may act by binding to nucleic acids. It displays two splice donor (SD) sites at nucleotides 106 and 191 (amino acids 35 and 64) that can act in concert with splice acceptors (SAs) in downstream genomic sequences to generate fusion proteins. ORF0 transcripts are reported to exist in induced pluripotent stem cells (iPSc) derived from primates. (Ref.: Denli, AM et al (2015). Cell 163(3): 583 593).
References
Product Information
FormatUnpurified
PresentationRabbit polyclonal antiserum with 0.05% sodium azide.
Applications
ApplicationAnti-ORF0 Antibody, Cat. No. ABE2895, is a highly specific rabbit polyclonal antibody, that targets and has been tested in Immunoprecipitation, Immunocytochemistry and Western Blotting.
Key Applications
  • Immunoprecipitation
  • Immunocytochemistry
  • Western Blotting
Application NotesWestern Blotting Analysis: A representative lot detected ORF0 in HEK293T cells transfected with ORF0 (Denli, A.M., et. al. (2015). Cell. 163(3):583-93).

Immunoprecipitation Analysis: A representative lot detected ORF0 in HEK293T cells transfected with ORF0 (Denli, A.M., et. al. (2015). Cell. 163(3):583-93).

Immunocytochemistry Analysis: A representative lot detected ORF0 in HEK293T cells transfected with ORF0 (Denli, A.M., et. al. (2015). Cell. 163(3):583-93).
Biological Information
ImmunogenKLH-conjugated linear peptides corresponding to ORF0 amino acid residues 20-34, 33-49, and 50-65.
ConcentrationPlease refer to lot specific datasheet.
HostRabbit
SpecificityThis rabbit polyclonal antibody recognizes primate-specific open reading frame 0 (ORF0).
IsotypeIgG
Species Reactivity
  • Primate
Species Reactivity NotePrimate. Predicted to react with Chimpanzee and Human based on 100% sequence homology.
Antibody TypePolyclonal Antibody
Gene Symbol
  • ORF0
Purification MethodUnpurified
UniProt Number
Molecular Weight16 kDa observed. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in extracts from HEK293T cells transfected with ORF0 expression plasmid. Western Blotting Analysis: A  1:5,000 dilution of this antibody detected ORF0 in lysate from HEK293T cells transfected with ORF0 expression plasmid.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at -20°C from date of receipt.
Packaging Information
Material Size25 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Katalogové číslo GTIN
ABE2895-25UL 04054839474958