Millipore Sigma Vibrant Logo
Attention: We have moved. Merck Millipore products are no longer available for purchase on MerckMillipore.com.Learn More

PC364 Anti-Netrin-1 (Ab-2) (497-508) Rabbit pAb

Anti-Netrin-1 (Ab-2) (497-508) Rabbit pAb MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
PC364
  
Purchase on Sigma-Aldrich

Přehled

Replacement Information

Tabulka spec. kláve

Host
Rb
Description
Overview

This product has been discontinued.





Recognizes the ~70 kDa netrin-1 protein in mouse brain tissue. Supplied with a control peptide.
Catalogue NumberPC364
Brand Family Calbiochem®
Application Data
Detection of mouse netrin-1 by staining frozen sections. Sample: Day 14 embryonic mouse brain tissue fixed with 4% paraformaldehyde. Primary antibody: Anti-Netrin-1 (Ab-2) (497-508) Rabbit pAb (Cat. No. PC364) (2 μg/ml).
References
ReferencesVarela-Echavarria, A. and Guthrie, S. 1997. Genes and Dev. 11, 545.
Harris, R., et al. 1996. Neuron 17, 217.
Keino-Masu, K., et al. 1996. Cell 87, 175.
Mitchell, K.J., et al. 1996. Neuron 17, 203.
Serafini, T., et al. 1996. Cell 87, 1001.
Tessier-Lavigne, M. and Goodman, C.S., 1996. Science 274, 1123.
Kennedy, T.E., et al. 1994. Cell 78, 425.
Serafini, T., et al. 1994. Cell 78, 409.
Ishii, N., et al. 1992. Neuron 9, 873.
Hedgecock, E.M., et al. 1990. Neuron 4, 61.
Product Information
FormLiquid
Formulation100 µg antibody in 0.05 M sodium phosphate buffer, 0.2% gelatin and 25 µg lyophilized control peptide (immunogen).
Positive controlMouse brain tissue
Preservative≤0.1% sodium azide (antibody only)
Applications
Key Applications Frozen Sections
Not Western Blot
Application NotesFrozen Sections (1-2 µg/ml)
Immunoblotting (not recommended)
Application CommentsNetrin-1 is enriched in embryonic neural tissue. Immunohistochemistry was performed using embryonic day 14 (E14) mouse brain sections fixed in 4% paraformaldehyde. Staining was completely abolished by preincubating the purified antibody with control peptide at 10-6M. Unconjugated Netrin-1 Control Peptide has been included for use as an internal control for antibody specificity. For immunoblotting applications, we recommend Netrin-1 (Ab-1) Polyclonal Antibody (Cat. No. PC344). Antibody should be titrated for optimal results in individual systems.
Biological Information
Immunogena synthetic peptide (QIHILKADKAGD) corresponding to amino acids 497-508 of mouse netrin-1, conjugated to KLH
ImmunogenMouse
HostRabbit
IsotypeIgG
Concentration Label Please refer to vial label for lot-specific concentration
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Blue Ice Only
Toxicity Standard Handling
Storage +2°C to +8°C
Do not freeze Yes
Special InstructionsStore lyophilized peptide at -20°C. Reconstitute peptide with 25 µl of dH₂O for a working stock of 1 mg/ml. Following reconstitution of peptide, aliquot and freeze (-20°C).
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Katalogové číslo GTIN
PC364 0

Documentation

Anti-Netrin-1 (Ab-2) (497-508) Rabbit pAb Certificates of Analysis

TitleLot Number
PC364

References

Přehled odkazů
Varela-Echavarria, A. and Guthrie, S. 1997. Genes and Dev. 11, 545.
Harris, R., et al. 1996. Neuron 17, 217.
Keino-Masu, K., et al. 1996. Cell 87, 175.
Mitchell, K.J., et al. 1996. Neuron 17, 203.
Serafini, T., et al. 1996. Cell 87, 1001.
Tessier-Lavigne, M. and Goodman, C.S., 1996. Science 274, 1123.
Kennedy, T.E., et al. 1994. Cell 78, 425.
Serafini, T., et al. 1994. Cell 78, 409.
Ishii, N., et al. 1992. Neuron 9, 873.
Hedgecock, E.M., et al. 1990. Neuron 4, 61.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision09-October-2007 RFH
ApplicationFrozen Sections (1-2 µg/ml)
Immunoblotting (not recommended)
Application Data
Detection of mouse netrin-1 by staining frozen sections. Sample: Day 14 embryonic mouse brain tissue fixed with 4% paraformaldehyde. Primary antibody: Anti-Netrin-1 (Ab-2) (497-508) Rabbit pAb (Cat. No. PC364) (2 μg/ml).
DescriptionPurified rabbit polyclonal antibody. Recognizes the ~70 kDa netrin-1 protein. Supplied with a control peptide (immunogen).
BackgroundIn the developing nervous system, axon guidance and the establishment of appropriate neuronal connections involves a number of chemoattractant and chemorepellent molecules. Netrins are related to the extracellular matrix protein laminin and comprise a family of secreted molecules capable of guiding the growth cone. The first netrin family member to be characterized, UNC-6 from C. elegans, was initially identified from the gene mutation which lead to defects in circumferential axon migration in the nematode. In contrast, the identification of the first vertebrate netrins, designated netrin-1 and netrin-2, resulted from a biochemical rather than genetic approach. Netrin homologs have subsequently been identified from a number of species. Results from several studies suggest a role for netrin-1 in mediating chemotropic and outgrowth-promoting activities of floor plate cells to guide commissural axons towards the ventral midline. DCC (Deleted in Colorectal Cancer) is believed to function as a component of a receptor complex which mediates the effects of netrin-1 on commissural axons and helps coordinate the navigation of axons to establish precise patterns of neural connectivity. In addition to the netrins and their receptors, a number of other diffusable or membrane-associated ligand families and their receptors have been implicated in axon guidance, including the semaphorins, ephrins, and cell adhesion molecules.
HostRabbit
Immunogen speciesMouse
Immunogena synthetic peptide (QIHILKADKAGD) corresponding to amino acids 497-508 of mouse netrin-1, conjugated to KLH
IsotypeIgG
Speciesmouse
Positive controlMouse brain tissue
FormLiquid
Formulation100 µg antibody in 0.05 M sodium phosphate buffer, 0.2% gelatin and 25 µg lyophilized control peptide (immunogen).
Concentration Label Please refer to vial label for lot-specific concentration
Preservative≤0.1% sodium azide (antibody only)
CommentsNetrin-1 is enriched in embryonic neural tissue. Immunohistochemistry was performed using embryonic day 14 (E14) mouse brain sections fixed in 4% paraformaldehyde. Staining was completely abolished by preincubating the purified antibody with control peptide at 10-6M. Unconjugated Netrin-1 Control Peptide has been included for use as an internal control for antibody specificity. For immunoblotting applications, we recommend Netrin-1 (Ab-1) Polyclonal Antibody (Cat. No. PC344). Antibody should be titrated for optimal results in individual systems.
Storage +2°C to +8°C
Do Not Freeze Yes
Special InstructionsStore lyophilized peptide at -20°C. Reconstitute peptide with 25 µl of dH₂O for a working stock of 1 mg/ml. Following reconstitution of peptide, aliquot and freeze (-20°C).
Toxicity Standard Handling
ReferencesVarela-Echavarria, A. and Guthrie, S. 1997. Genes and Dev. 11, 545.
Harris, R., et al. 1996. Neuron 17, 217.
Keino-Masu, K., et al. 1996. Cell 87, 175.
Mitchell, K.J., et al. 1996. Neuron 17, 203.
Serafini, T., et al. 1996. Cell 87, 1001.
Tessier-Lavigne, M. and Goodman, C.S., 1996. Science 274, 1123.
Kennedy, T.E., et al. 1994. Cell 78, 425.
Serafini, T., et al. 1994. Cell 78, 409.
Ishii, N., et al. 1992. Neuron 9, 873.
Hedgecock, E.M., et al. 1990. Neuron 4, 61.