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MABC1038 Anti-NQO2 Antibody, clone 4A4.1

Anti-NQO2 Antibody, clone 4A4.1 is an antibody against NQO2 for use in Western Blotting, Immunohistochemistry.

Anti-NQO2 Antibody, clone 4A4.1 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Apoptosis & Cancer Research Sub-Category: Oxidative Stress Gene Symbol: NQO2, NMOR2 UniProt Number: P16083
MABC1038
100 μg  
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Species ReactivityKey ApplicationsHostFormatAntibody Type
HWB, IHCMPurifiedMonoclonal Antibody
Description
Catalogue NumberMABC1038
DescriptionAnti-NQO2 Antibody, clone 4A4.1
Alternate Names
  • Ribosyldihydronicotinamide dehydrogenase [quinone]
  • NRH dehydrogenase [quinone] 2
  • NRH:quinone oxidoreductase 2
  • Quinone reductase 2
  • QR2
Background InformationRibosyldihydronicotinamide dehydrogenase [quinone] (EC 1.10.5.1; UniProt P16083; also known as NRH dehydrogenase [quinone] 2, NRH:quinone oxidoreductase 2, Quinone reductase 2, QR2) is encoded by the NQO2 (also known as NMOR2) gene (Gene ID 4835) in human. NQO1 (EC 1.6.5.2) and NQO2 (EC 1.10.5.1; formerly EC 1.10.99.2) constitute the quinone oxidoreductase family in human. NQO1 is involved in vitamin K metabolism and in reducing the cellular quinone concentration to prevent the build-up of cellular reactive oxygen species (ROS). Both enzymes contain a tightly bound FAD as the oxidant cofactor, but differ in their reductant cofactors utilization. While NQO1 can efficiently utilize NADH or NADPH, NQO2 exhibits only low activity in the presence of NADH or NADPH. Instead, non-physiological compounds, such as dihydronicotinamide riboside (NRH), N-methyldihydronicotinamide, and dihydrobenzylnicotamide, are reported to act as good reductant cofactors in NQD2-catalyzed reactions in vitro. NQO1 & NQO2 catalyzed the reduction of a wide variety of substrates, including quinones, nitro-compounds, and iron (III) ions. NQO1 is inhibited by the anticoagulant dicoumarol, while NQO2 is only weakly inhibited by dicoumarol and more strongly inhibited by resveratrol.
References
Product Information
FormatPurified
PresentationPurified mouse monoclonal IgG2bκ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationAnti-NQO2 Antibody, clone 4A4.1 is an antibody against NQO2 for use in Western Blotting, Immunohistochemistry.
Key Applications
  • Western Blotting
  • Immunohistochemistry
Application NotesWestern Blotting Analysis: 0.5 µg/mL from a representative lot detected NQO2 in 10 µg of Jurkat cell lysate.
Immunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected NQO2 in human kidney, liver, and cerebral cortex tissue sections.
Biological Information
ImmunogenGST-tagged recombinant human NQO2 N-terminal fragment.
EpitopeInternal (N-terminal half).
Clone4A4.1
ConcentrationPlease refer to lot specific datasheet.
HostMouse
SpecificityClone 4A4.1 recognizes an internal epitope within the N-terminal half of human Quinone reductase 2.
IsotypeIgG2bκ
Species Reactivity
  • Human
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • NQO2
  • NMOR2
Purification MethodProtein G Purified
UniProt Number
Molecular Weight~26 kDa observed. Uncharacterized band(s) may appear in some lysates.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in HeLa cell lysate.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected NQO2 in 10 µg of HeLa cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Katalogové číslo GTIN
MABC1038 04055977333213