Millipore Sigma Vibrant Logo
Attention: We have moved. Merck Millipore products are no longer available for purchase on MerckMillipore.com.Learn More

MABN229 Anti-NOS1AP Antibody, clone 6E1.1

Anti-NOS1AP Antibody, clone 6E1.1 is an antibody against NOS1AP for use in Western Blotting, Immunohistochemistry .

Anti-NOS1AP Antibody, clone 6E1.1 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Neuroscience Research Sub-Category: Developmental Signaling Gene Symbol: NOS1AP, CAPON, KIAA0464 UniProt Number: O75052
MABN229
100 μg  
Purchase on Sigma-Aldrich

Speciální nabídky

Přehled

Replacement Information

Speciální nabídky

100% Performance Guaranteed

Tabulka spec. kláve

Species ReactivityKey ApplicationsHostFormatAntibody Type
H, R, MWB, IH(P)MPurifiedMonoclonal Antibody
Description
Catalogue NumberMABN229
DescriptionAnti-NOS1AP Antibody, clone 6E1.1
Alternate Names
  • Carboxyl-terminal PDZ ligand of neuronal nitric oxide synthase protein
  • C-terminal PDZ ligand of neuronal nitric oxide synthase protein
  • Nitric oxide synthase 1 adaptor protein
  • NOS1AP
Background InformationCarboxyl-terminal PDZ ligand of neuronal nitric oxide synthase(nNOS) protein or CAPON (UniProt O75052), also known as Nitric oxide synthase (NOS) 1 adaptor protein, C-terminal PDZ ligand of nNOS, C-terminal PDZ domain ligand of nNOS, Ligand of nNOS with carboxyl-terminal PDZ domain) is a cytosolic protein encoded by the NOS1AP gene (Entrez Gene ID 9722) in human. NOS1AP protein (CAPON) contains a C-terminal PDZ-binding domain and an N-terminal phosphotyrosine binding (PTB) domain that mediates its interactions with nNOS and the small monomeric G protein Dexras1, respectively. Originally it was characterized as a competitor against PSD95 for nNOS interaction, but later evidence indicates that nNOS-CAPON association contributes to the modulation of anxiety-related behavior via regulating Dexras1-ERK signaling. CAPON is also reported to co-localize with the L-type calcium channel and the inwardly rectifying potassium channel Kir3.1 in murine cardiac myocytes. In addition, CAPON is significantly upregulated in mice with dystrophic cardiomyopathy, implicating a role of NOS1AP protein on cardiac arrhythmias, most likely through S-nitrosylation of L-type calcium channel and potassium channels.
References
Product Information
FormatPurified
PresentationPurified mouse monoclonal IgG2bκ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ300
Applications
ApplicationAnti-NOS1AP Antibody, clone 6E1.1 is an antibody against NOS1AP for use in Western Blotting, Immunohistochemistry .
Key Applications
  • Western Blotting
  • Immunohistochemistry (Paraffin)
Application NotesWestern Blotting Analysis: 1.0 µg/mL from a representative lot detected NOS1AP in 200 µg of human brain, mouse brain, and human cerebellum tissue lysate.
Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected NOS1AP in human cerebellum, rat cerebellum, human cerebral cortex, and rat cerebral cortex tissue.
Biological Information
ImmunogenGST-tagged recombinant protein corresponding to the C-terminal of human NOS1AP.
EpitopeC-terminal
Clone6E1.1
ConcentrationPlease refer to lot specific datasheet.
HostMouse
IsotypeIgG2bκ
Species Reactivity
  • Human
  • Rat
  • Mouse
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • NOS1AP
  • CAPON
  • KIAA0464
Purification MethodProtein G Purified
UniProt Number
Molecular Weight~53 kDa observed. Uncharacterized band(s) may appear in some lysates.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in rat brain tissue lysate.

Western Blotting Analysis: 1.0 µg/mL of this antibody detected NOS1AP in 200 µg of rat brain tissue lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Katalogové číslo GTIN
MABN229 04055977168297