Millipore Sigma Vibrant Logo
Attention: We have moved. Merck Millipore products are no longer available for purchase on MerckMillipore.com.Learn More

ST1119 Anti-NOD2/CARD15 Rabbit pAb

Anti-NOD2/CARD15 Rabbit pAb MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Anti-BLAU, Anti-CARD15, Anti-ACUG

ST1119
  
Purchase on Sigma-Aldrich

Přehled

Replacement Information

Tabulka spec. kláve

Host
Rb
Description
Overview

This product has been discontinued.





Recognizes the ~150 kDa NOD2 protein in Jurkat cells.
Catalogue NumberST1119
Brand Family Calbiochem®
SynonymsAnti-BLAU, Anti-CARD15, Anti-ACUG
Application Data
Lane 1: Detection of human NOD2 by immunoblotting. Sample: Whole cell lysate (20 µg) from Jurkat cells. Primary antibody: Anti-NOD2 Rabbit pAb (Cat. No. ST1119) (2 µg/ml). Detection: chemiluminescence. Lane 2. Detection of human NOD2 by immunoprecipitation followed by immunoblotting. Sample: Whole cell lysate (500 µg) from Jurkat cells (lane 2) and negative control sample (lane 3). Antibody for immunoprecipitation: Anti-NOD2 Rabbit pAb (Cat. No. ST1119) (5 µg). Immunoblotting conditions: same as lane 1.
References
ReferencesFritz, J.H., et al. 2006. Nat. Immunol. 7, 1250.
Radford-Smith, G. and Pandeya, N. 2006. World J. Gastroenterol. 12, 7097.
Product Information
FormLiquid
FormulationIn Tris-citrate/phosphate buffer, pH 7.0-8.0.
Preservative≤0.1% sodium azide
Applications
Key Applications Immunoblotting (Western Blotting)
Immunoprecipitation
Application NotesImmunoblotting (2 µg/ml)
Immunoprecipitation (5 µg/500 µg total protein)
Application CommentsAntibody should be titrated for optimal results in individual systems.
Biological Information
Immunogena synthetic peptide corresponding to amino acids from human NOD2
ImmunogenHuman
HostRabbit
IsotypeIgG
Concentration Label Please refer to vial label for lot-specific concentration
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Blue Ice Only
Toxicity Standard Handling
Storage -20°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Katalogové číslo GTIN
ST1119 0

Documentation

References

Přehled odkazů
Fritz, J.H., et al. 2006. Nat. Immunol. 7, 1250.
Radford-Smith, G. and Pandeya, N. 2006. World J. Gastroenterol. 12, 7097.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision25-October-2007 RFH
SynonymsAnti-BLAU, Anti-CARD15, Anti-ACUG
ApplicationImmunoblotting (2 µg/ml)
Immunoprecipitation (5 µg/500 µg total protein)
Application Data
Lane 1: Detection of human NOD2 by immunoblotting. Sample: Whole cell lysate (20 µg) from Jurkat cells. Primary antibody: Anti-NOD2 Rabbit pAb (Cat. No. ST1119) (2 µg/ml). Detection: chemiluminescence. Lane 2. Detection of human NOD2 by immunoprecipitation followed by immunoblotting. Sample: Whole cell lysate (500 µg) from Jurkat cells (lane 2) and negative control sample (lane 3). Antibody for immunoprecipitation: Anti-NOD2 Rabbit pAb (Cat. No. ST1119) (5 µg). Immunoblotting conditions: same as lane 1.
DescriptionImmunoaffinity purified rabbit polyclonal antibody. Recognizes the ~150 kDa NOD2/CARD15 protein.
BackgroundNod2 (nucleotide-binding oligomerization domain containing 2; accession no. NP 071445) is a 150 kDa protein that is a member of the Nod-like receptor (NLR) family. It is strongly associated with host responses to intracellular invasion by bacteria and inflammatory responses. Mutations in Nod2 have been associated with the inflammatory disorders, Crohn's disease and Blau syndrome. Nod2 contains two caspase recruitment (CARD) domains at the N-terminus, a NACHT domain in the central region, and six leucine-rich repeats (LRRs) at the C-terminus. Bacterial pathogens are recognized by the host cells extracellularly through Toll-like receptors or intracellularly by a family of cytosolic Nod-like receptors (NLRs). NLRs recognize the microbial molecules that are introduced by the pathogens and/or endogenous factors released from damaged tissue. Nod2 detects intracellular presence of muramyl dipeptide, the largest molecular motif common to Gram-negative and Gram-positive bacteria. After detection of the bacterial product, Nod2 forms a complex with receptor-interacting protein 2 (RIP2) through a CARD-CARD interaction. This complex recruits NEMO (IKK-γ) and mediates the ubiquitin-proteasome-dependent degradation of IκBα, which results in the activation and nuclear traslocation of NF-κB and the upregulation of inflammatory mediators.
HostRabbit
Immunogen speciesHuman
Immunogena synthetic peptide corresponding to amino acids from human NOD2
IsotypeIgG
Specieshuman
FormLiquid
FormulationIn Tris-citrate/phosphate buffer, pH 7.0-8.0.
Concentration Label Please refer to vial label for lot-specific concentration
Preservative≤0.1% sodium azide
CommentsAntibody should be titrated for optimal results in individual systems.
Storage Avoid freeze/thaw
-20°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Toxicity Standard Handling
ReferencesFritz, J.H., et al. 2006. Nat. Immunol. 7, 1250.
Radford-Smith, G. and Pandeya, N. 2006. World J. Gastroenterol. 12, 7097.