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AP1166 Anti-NGFRAP1 Mouse mAb (4E5)

This Anti-NGFRAP1 Mouse mAb (4E5) is validated for use in ELISA, Immunoblotting for the detection of NGFRAP1.

Anti-NGFRAP1 Mouse mAb (4E5) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Anti-NADE, Anti-BEX3, Anti-Nerve Growth Factor Receptor Associated Protein 1

AP1166
  
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Replacement Information

Tabulka spec. kláve

Species ReactivityHostAntibody Type
HMMonoclonal Antibody
Description
OverviewRecognizes NGFRAP1 protein.
Catalogue NumberAP1166
Brand Family Calbiochem®
SynonymsAnti-NADE, Anti-BEX3, Anti-Nerve Growth Factor Receptor Associated Protein 1
Application Data
Detection of human NGFRAP1 by immunoblotting. Sample: Partial recombinant protein. Primary antibody: Anti-NGFRAP1 Mouse mAb (4E5) (Cat. No. AP1166) (1-5 µg/ml). Detection: chemiluminescence.
References
Product Information
FormLiquid
FormulationIn PBS, pH 7.2.
PreservativeNone
Applications
Key Applications Enzyme-Linked Immunosorbent Assay
Immunoblotting (Western Blotting)
Application NotesELISA (see comments)
Immunoblotting (1-5 µg)
Application CommentsFor ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
Biological Information
ImmunogenA recombinant polypeptide corresponding to amino acids of 1-110 human NGFRAP1, expressed as a GST fusion protein
Clone4E5
HostMouse
IsotypeIgG2b
Species Reactivity
  • Human
Antibody TypeMonoclonal Antibody
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Blue Ice Only
Toxicity Regulatory Review
Storage -20°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Katalogové číslo GTIN
AP1166 0

Documentation

Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision19-October-2010 RFH
SynonymsAnti-NADE, Anti-BEX3, Anti-Nerve Growth Factor Receptor Associated Protein 1
ApplicationELISA (see comments)
Immunoblotting (1-5 µg)
Application Data
Detection of human NGFRAP1 by immunoblotting. Sample: Partial recombinant protein. Primary antibody: Anti-NGFRAP1 Mouse mAb (4E5) (Cat. No. AP1166) (1-5 µg/ml). Detection: chemiluminescence.
DescriptionPurified mouse monoclonal antibody. Recognizes the NGFRAP1 protein.
BackgroundThe p75 neurotrophin receptor (p75NTR) is a member of the tumor necrosis receptor superfamily and can mediate cell death and cell survival in response to nerve growth factor (NGF). The p75NTR-associated cell death executor (NADE) mediates apoptosis by interacting with the cell death domain of p75NTR following the binding of NGF by p75NTR. Recent studies have shown that NADE also interacts with second mitochondria-derived activator of caspase (Smac). Coexpression of NADE and Smac promotes TRAIL-induced apoptosis and inhibits XIAP-mediated Smac ubiquitization. It has been suggested that it is this interaction between NADE and Smac that allows apoptosis to proceed.
HostMouse
ImmunogenA recombinant polypeptide corresponding to amino acids of 1-110 human NGFRAP1, expressed as a GST fusion protein
Clone4E5
IsotypeIgG2b
Specieshuman
FormLiquid
FormulationIn PBS, pH 7.2.
PreservativeNone
CommentsFor ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
Storage Avoid freeze/thaw
-20°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Toxicity Regulatory Review