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CA1029 Anti-N-Cadherin Mouse mAb (13A9)

Anti-N-Cadherin Mouse mAb (13A9) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
CA1029
  
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Přehled

Replacement Information

Tabulka spec. kláve

Host
M
Description
Overview

This product has been discontinued.





Recognizes the ~140 kDa N-cadherin protein in HeLa cells.
Catalogue NumberCA1029
Brand Family Calbiochem®
Application Data
Lane 1: Detection of human N-cadherin by immunoblotting. Sample: Whole cell lysate (25 µg) from HeLa cells. Primary antibody: Anti-N-Cadherin Mouse mAb (13A9) (Cat. No. CA1029) (1:1000). Detection: chemiluminescence. Lane 2: Detection of human N-cadherin by immunoprecipitation followed by immunoblotting. Antibody for immunoprecipitation: Anti N-Cadherin Mouse mAb (13A9) (Cat No. CA1029) (1:100/500 µg total protein). Immunoblotting conditions: same as lane 1.
References
ReferencesChristofori, G. 2006. Nature 44, 444.
Cvallaro, U., et al. 2006. Exp. Cell Res. 312, 659.
Cowin, P., et al. 2005. Curr. Op. Cell Biol. 17, 499.
Product Information
FormLiquid
FormulationUndiluted ascites.
Positive controlHeLa cells
Preservative≤0.1% sodium azide
Applications
Key Applications Immunoblotting (Western Blotting)
Immunoprecipitation
Application NotesImmunoblotting (1:1000)
Immunoprecipitation (1:100)
Application CommentsAntibody should be titrated for optimal results in individual systems.
Biological Information
Immunogena recombinant fusion protein consisting of the cytoplasmic domain of human N-cadherin, expressed in E. coli
ImmunogenHuman
Clone13A9
HostMouse
IsotypeIgG
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Blue Ice Only
Toxicity Standard Handling
Storage -20°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Katalogové číslo GTIN
CA1029 0

Documentation

References

Přehled odkazů
Christofori, G. 2006. Nature 44, 444.
Cvallaro, U., et al. 2006. Exp. Cell Res. 312, 659.
Cowin, P., et al. 2005. Curr. Op. Cell Biol. 17, 499.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision20-August-2007 RFH
ApplicationImmunoblotting (1:1000)
Immunoprecipitation (1:100)
Application Data
Lane 1: Detection of human N-cadherin by immunoblotting. Sample: Whole cell lysate (25 µg) from HeLa cells. Primary antibody: Anti-N-Cadherin Mouse mAb (13A9) (Cat. No. CA1029) (1:1000). Detection: chemiluminescence. Lane 2: Detection of human N-cadherin by immunoprecipitation followed by immunoblotting. Antibody for immunoprecipitation: Anti N-Cadherin Mouse mAb (13A9) (Cat No. CA1029) (1:100/500 µg total protein). Immunoblotting conditions: same as lane 1.
DescriptionMouse monoclonal antibody supplied as undiluted ascites. Recognizes the ~140 kDa N-cadherin protein.
BackgroundCadherins are a large family of Ca2+-dependant cell adhesion transmembrane glycoproteins that play a role in cell to cell recognition. The classic cadherins include N-, R-, P-, B-, and E-cadherin. Although cadherins interact with similar intracellular proteins, they can exert specifc activities. Early non-invasive epithelial cells express E-cadherin. However, during the the process of epithelial to mesenchymal transition (EMT), the expression of E-cadherin is lost and the expression of N-cadherin is upregulated. This process, the cadherin switch, results in the transition of a benign tumor to a malignant and metastatic form. N-cadherin is a 140-kDa protein that has several functions that contribute to tumor invasion. It promotes cell-cell adhesion to N-cadherin stromal cells and it binds to and activates FGFRs that result in activation of the PI 3-K and MAPK pathways. N-cadherin is also cleaved by MMPs and a γ-secretase-like protease, releasing the extracellular domain of N-cadherin and the intracellular carboxy terminal.
HostMouse
Immunogen speciesHuman
Immunogena recombinant fusion protein consisting of the cytoplasmic domain of human N-cadherin, expressed in E. coli
Clone13A9
IsotypeIgG
Specieshuman
Positive controlHeLa cells
FormLiquid
FormulationUndiluted ascites.
Preservative≤0.1% sodium azide
CommentsAntibody should be titrated for optimal results in individual systems.
Storage Avoid freeze/thaw
-20°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Toxicity Standard Handling
ReferencesChristofori, G. 2006. Nature 44, 444.
Cvallaro, U., et al. 2006. Exp. Cell Res. 312, 659.
Cowin, P., et al. 2005. Curr. Op. Cell Biol. 17, 499.