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MABS2047-25UG Anti-MT-ND2 Antibody, clone 9E12-1B3

Anti-MT-ND2, clone 9E12-1B3, Cat. No. MABS2047, is a mouse monoclonal antibody that detects NADH-ubiquinone oxidoreductase chain 2 and has been tested for use in Western Blotting.

Anti-MT-ND2 Antibody, clone 9E12-1B3 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Signaling Gene Symbol: MT-ND2, MTND2, NADH2, ND2 UniProt Number: P03891
MABS2047-25UG
25 μg  
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Tabulka spec. kláve

Species ReactivityKey ApplicationsHostFormatAntibody Type
HWBMPurifiedMonoclonal Antibody
Description
Catalogue NumberMABS2047-25UG
DescriptionAnti-MT-ND2 Antibody, clone 9E12-1B3
Alternate Names
  • NADH-ubiquinone oxidoreductase chain 2
  • EC: 1.6.5.3
  • NADH dehydrogenase subunit 2
  • MT-ND2
Background InformationNADH-ubiquinone oxidoreductase chain 2 (UniProt: P03891; also known as EC: 1.6.5.3, NADH dehydrogenase subunit 2, MT-ND2) is encoded by the MT-ND2 (also known as MTND2, NADH2, ND2) gene (Gene ID: 4536) in human. MT-ND2 is a mitochondrial inner membrane protein that forms the core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I). It is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone (Coenzyme Q10) that participates in the generation of a proton gradient, which is then used for ATP synthesis. Defects in MT-ND2 gene are known to cause of Leber hereditary optic neuropathy (LHON) that is a maternally inherited disease resulting in acute or subacute loss of central vision, due to optic nerve dysfunction. LHON results from primary mitochondrial DNA mutations affecting the respiratory chain complexes.
References
Product Information
FormatPurified
PresentationPurified mouse monoclonal antibody IgG2a in buffer containing HEPES-Buffered Saline (150 mM NaCl, 15 mM HEPES, pH 7.2) with 0.02% sodium azide.
Applications
ApplicationAnti-MT-ND2, clone 9E12-1B3, Cat. No. MABS2047, is a mouse monoclonal antibody that detects NADH-ubiquinone oxidoreductase chain 2 and has been tested for use in Western Blotting.
Key Applications
  • Western Blotting
Application NotesWestern Blotting Analysis: 2 µg/mL from a representative lot detected MT-ND2 in mitochondria from human neonatal dermal fibroblasts and mitochondria from human neonatal dermal fibroblasts depleted of mtDNA (Courtesy of Michael F. Marusich, Ph.D., mAbDx, Inc., Eugene, OR USA).
Biological Information
ImmunogenSynthetic peptide corresponding to the N-terminus of human MT-ND2.
Clone9E12-1B3
ConcentrationPlease refer to lot specific datasheet.
HostMouse
SpecificityClone 9E12-1B3 detects NADH-ubiquinone oxidoreductase chain 2 (MT-ND2) in mitochondria from human cells. It targets an epitope within the N-terminal region.
IsotypeIgG2aκ
Species Reactivity
  • Human
Species Reactivity NoteHuman.
Antibody TypeMonoclonal Antibody
Gene Symbol
  • MT-ND2
  • MTND2
  • NADH2
  • ND2
Purification MethodProtein L
UniProt Number
Molecular Weight~30 kDa observed; 38.96 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in mitochondria from human neonatal dermal fibroblasts and mitochondria from human neonatal dermal fibroblasts depleted of mtDNA.
Western Blotting Analysis: 1 ug/mL of this antibody detected MT-ND2 in 10 µg of human neonatal dermal fibroblasts and mitochondria from human neonatal dermal fibroblasts depleted of mtDNA.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size25 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Katalogové číslo GTIN
MABS2047-25UG 04054839550959