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MABT456 Anti-MSN Antibody, clone 2C12

Detect Moesin using this mouse monoclonal antibody, Anti-MSN Antibody, clone 2C12 validated for use in western blotting, IHC & Flow Cytometry.

Anti-MSN Antibody, clone 2C12 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Inflammation & Immunology Research Sub-Category: Immunological Signaling Gene Symbol: MSN UniProt Number: P26038
MABT456
100 µL  
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Tabulka spec. kláve

Species ReactivityKey ApplicationsHostFormatAntibody Type
HWB, IHC, FCMAscitesMonoclonal Antibody
Description
Catalogue NumberMABT456
DescriptionAnti-MSN Antibody, clone 2C12
Alternate Names
  • Moesin
  • Membrane-organizing extension spike protein
Background InformationMoesin (membrane-organizing extension spike protein) is the protein encoded by the MSN gene. The MSN encoded protein moesin is a member of the ERM (ezrin, moesin, radixin) family of proteins that regulate linkage between the plasma membrane and the actin cytoskeleton. Thus, the MSN encoded protein is a peripheral membrane protein that is keenly involved in the signaling and structural connections in cytoskeletal plasma membrane structures like microvilli. Moesin in its active, phosphorylated state strongly binds actin and helps anchor it to the plasma membrane. The MSN encoded Moesin is localized to filopodia and other membranous extensions of various cell types and thus plays a role in cell-cell recognition signaling, cell movement, and cell transport. In its inactive, non-phosphorylated state, Moesin binds itself in a head-tail fashion and is incapable of binding actin or other cytoskeletal proteins. The kinase ROCK2 is central to the opening up of moesin and allowing its interaction with actin and the plasma membrane. EMD-Millipore’s Anti-MSN, clone 2C12 has been tested in western blots against purified recombinant protein as well as multiple human cancer cell lysates. The clone has also been tested successfully in paraffin embedded immunohistochemistry on human colon tissue and by flow cytometry on human Jurkat cells and ELISA using purified recombinant antigen.
References
Product Information
FormatAscites
Control
  • Jurkat cell lysate
PresentationMouse monoclonal IgG1 ascitic fluid containing up to 0.1% sodium azide.
Quality LevelMQ100
Applications
ApplicationDetect Moesin using this mouse monoclonal antibody, Anti-MSN Antibody, clone 2C12 validated for use in western blotting, IHC & Flow Cytometry.
Key Applications
  • Western Blotting
  • Immunohistochemistry
  • Flow Cytometry
Application NotesImmunohistochemistry Analysis: A 1:100-1,000 dilution from a representative lot detected MSN in human tonsil and colon tissues.

Flow Cytometry Analysis: A 1:200-400 dilution from a representative lot detected MSN in Jurkat cells.

Optimal working dilutions must be determined by end user.
Biological Information
ImmunogenPurified protein fragment of human MSN expressed in E. coli.
Clone2C12
HostMouse
IsotypeIgG1
Species Reactivity
  • Human
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • MSN
Purification MethodUnpurified
UniProt Number
Molecular Weight~68 kDa observed. Uncharacterized bands may appear in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in Jurkat cell lysate.

Western Blotting Analysis: A 1:500 dilution of this antibody detected MSN in 10 µg of Jurkat cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Katalogové číslo GTIN
MABT456 04053252910289