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NA28 Anti-MLH1 (Ab-1) Mouse mAb (14)

Anti-MLH1 (Ab-1) Mouse mAb (14) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
NA28
  
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Přehled

Replacement Information

Tabulka spec. kláve

Species ReactivityHostAntibody Type
HMMonoclonal Antibody
Description
Overview

This product has been discontinued.



Recognizes the ~83 kDa MLH1 protein in LoVo cells.
Catalogue NumberNA28
Brand Family Calbiochem®
References
ReferencesKane, M.F., et al. 1997. Cancer Res. 57, 808.
Bronner, C.E., et al. 1994. Nature 368, 258.
Papadopoulos, N., et al. 1994. Science 263, 1625.
Peltomäki, P.T. 1994. Annals of Medicine 26, 215.
Fishel, R., et al. 1993. Cell 75, 1027.
Leach, F.S., et al. 1993. Cell 75, 1215.
Lindbolm, A., et al. 1993. Nature Genetics 5, 279.
Product Information
FormLiquid
FormulationIn 0.05 M sodium phosphate buffer, 0.2% gelatin.
Negative controlHCT116 cells
Positive controlLoVo cells or colon tissue
Preservative≤0.1% sodium azide
Quality LevelMQ100
Applications
Key Applications Immunoblotting (Western Blotting)
Paraffin Sections
Application NotesImmunoblotting (1 µg/ml)
Paraffin Sections (10 µg/ml, heat pre-treatment required)
Application CommentsFor paraffin sections the antibody may give a weak immunoreactive staining pattern; high-pressure heat-induced antigen retrieval method is recommended using high/low pH citrate buffer. Antibody should be titrated for optimal results in individual systems.
Biological Information
Immunogenrecombinant, human MLH1
ImmunogenHuman
Clone14
HostMouse
IsotypeIgG₁
Species Reactivity
  • Human
Antibody TypeMonoclonal Antibody
Concentration Label Please refer to vial label for lot-specific concentration
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Blue Ice Only
Toxicity Standard Handling
Storage -20°C
Do not freeze Yes
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Katalogové číslo GTIN
NA28 0

Documentation

Anti-MLH1 (Ab-1) Mouse mAb (14) MSDS

Title

Safety Data Sheet (SDS) 

Anti-MLH1 (Ab-1) Mouse mAb (14) Certificates of Analysis

TitleLot Number
NA28

References

Přehled odkazů
Kane, M.F., et al. 1997. Cancer Res. 57, 808.
Bronner, C.E., et al. 1994. Nature 368, 258.
Papadopoulos, N., et al. 1994. Science 263, 1625.
Peltomäki, P.T. 1994. Annals of Medicine 26, 215.
Fishel, R., et al. 1993. Cell 75, 1027.
Leach, F.S., et al. 1993. Cell 75, 1215.
Lindbolm, A., et al. 1993. Nature Genetics 5, 279.

Brochure

Title
Caspases and other Apoptosis Related Tools Brochure
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision05-September-2007 RFH
ApplicationImmunoblotting (1 µg/ml)
Paraffin Sections (10 µg/ml, heat pre-treatment required)
DescriptionPurified mouse monoclonal antibody generated by immunizing BALB/c mice with the specified immunogen and fusing splenocytes with SP2/0 mouse myeloma cells. Recognizes the ~87 kDa MLH1 protein.
BackgroundThe MLH1 gene is one of at least 4 genes encoding proteins involved in the repair of mismatch nucleotides following DNA replication or repair. Mutations in the MLH1 gene contribute to the development of sporadic colorectal carcinoma, while germline MLH1 mutations are responsible for ~35% of inherited, non-polyposis colorectal carcinoma (HNPCC). MLH1 encodes a protein of 756 amino acids.
HostMouse
Immunogen speciesHuman
Immunogenrecombinant, human MLH1
Clone14
IsotypeIgG₁
Specieshuman, not mouse
Positive controlLoVo cells or colon tissue
Negative controlHCT116 cells
FormLiquid
FormulationIn 0.05 M sodium phosphate buffer, 0.2% gelatin.
Concentration Label Please refer to vial label for lot-specific concentration
Preservative≤0.1% sodium azide
CommentsFor paraffin sections the antibody may give a weak immunoreactive staining pattern; high-pressure heat-induced antigen retrieval method is recommended using high/low pH citrate buffer. Antibody should be titrated for optimal results in individual systems.
Storage -20°C
Do Not Freeze Yes
Toxicity Standard Handling
ReferencesKane, M.F., et al. 1997. Cancer Res. 57, 808.
Bronner, C.E., et al. 1994. Nature 368, 258.
Papadopoulos, N., et al. 1994. Science 263, 1625.
Peltomäki, P.T. 1994. Annals of Medicine 26, 215.
Fishel, R., et al. 1993. Cell 75, 1027.
Leach, F.S., et al. 1993. Cell 75, 1215.
Lindbolm, A., et al. 1993. Nature Genetics 5, 279.