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OP144 Anti-MDM2 (Ab-4) Mouse mAb (2A9C1.18)

Anti-MDM2 (Ab-4) Mouse mAb (2A9C1.18) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Anti-Ubiquitin Protein Ligase, Anti-p53 Binding Protein, Ant-Murine Double Minute Chromosome-2

OP144
  
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Přehled

Replacement Information

Tabulka spec. kláve

Species ReactivityHostAntibody Type
H, MMMonoclonal Antibody
Description
Overview

This product has been discontinued.



Recognizes the ~90 kDa (apparent MW) MDM2 protein in A549 cells.
Catalogue NumberOP144
Brand Family Calbiochem®
SynonymsAnti-Ubiquitin Protein Ligase, Anti-p53 Binding Protein, Ant-Murine Double Minute Chromosome-2
Application Data
Detection of human MDM2 by staining paraffin sections. Sample: Human breast carcinoma, paraffin-embedded. Primary antibody: Anti-MDM2 (Ab-4) Mouse mAb (2A9C1.18) (Cat. No. OP144) (2.5 μg/ml). Detection: DAB.
References
ReferencesMarchetti, A., et al. 1995. J. Pathol. 175, 31.
Barak, Y., et al. 1993. EMBO. J. 12, 461.
Ladanyi, M., et al. 1993. Cancer Res. 53, 16.
Leach, F. S., et al. 1993. Cancer Res. 53, 2231.
Oliner, J.D., et al. 1993. Nature 362, 857.
Momand, J., et al. 1992. Cell 69, 1237.
Oliner, J.D., et al. 1992. Nature 358, 80.
Fakharzadeh, S.S., et al. 1991. EMBO J. 10, 1565.
Product Information
FormLiquid
FormulationIn 50 mM sodium phosphate buffer, 50% glycerol.
Positive controlA549 cells or most tissues
PreservativeNone
Quality LevelMQ100
Applications
Key Applications Immunoblotting (Western Blotting)
Immunofluorescence
Immunoprecipitation
Paraffin Sections
Application NotesImmunoblotting (2 µg/ml, chemiluminescence)
Immunofluorescence (1 µg/ml)
Immunoprecipitation (1 µg/reaction)
Paraffin Sections (2.5 µg/ml, heat pre-treatment required)
Application CommentsAntibody should be titrated for optimal results in individual systems.
Biological Information
Immunogenrecombinant, human MDM2
ImmunogenHuman
Epitopewithin amino acids 153-222
Clone2A9C1.18
HostMouse
IsotypeIgG2a
Species Reactivity
  • Human
  • Mouse
Antibody TypeMonoclonal Antibody
Concentration Label Please refer to vial label for lot-specific concentration
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Dry Ice Only
Toxicity Standard Handling
Storage -20°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Katalogové číslo GTIN
OP144 0

Documentation

Anti-MDM2 (Ab-4) Mouse mAb (2A9C1.18) MSDS

Title

Safety Data Sheet (SDS) 

Anti-MDM2 (Ab-4) Mouse mAb (2A9C1.18) Certificates of Analysis

TitleLot Number
OP144

References

Přehled odkazů
Marchetti, A., et al. 1995. J. Pathol. 175, 31.
Barak, Y., et al. 1993. EMBO. J. 12, 461.
Ladanyi, M., et al. 1993. Cancer Res. 53, 16.
Leach, F. S., et al. 1993. Cancer Res. 53, 2231.
Oliner, J.D., et al. 1993. Nature 362, 857.
Momand, J., et al. 1992. Cell 69, 1237.
Oliner, J.D., et al. 1992. Nature 358, 80.
Fakharzadeh, S.S., et al. 1991. EMBO J. 10, 1565.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision02-October-2007 RFH
SynonymsAnti-Ubiquitin Protein Ligase, Anti-p53 Binding Protein, Ant-Murine Double Minute Chromosome-2
ApplicationImmunoblotting (2 µg/ml, chemiluminescence)
Immunofluorescence (1 µg/ml)
Immunoprecipitation (1 µg/reaction)
Paraffin Sections (2.5 µg/ml, heat pre-treatment required)
Application Data
Detection of human MDM2 by staining paraffin sections. Sample: Human breast carcinoma, paraffin-embedded. Primary antibody: Anti-MDM2 (Ab-4) Mouse mAb (2A9C1.18) (Cat. No. OP144) (2.5 μg/ml). Detection: DAB.
DescriptionPurified mouse monoclonal antibody. Recognizes the ~90 kDa (apparent MW) MDM2 protein.
BackgroundIncreased expression of proto-oncogenes due to gene amplification can lead to cellular transformation in the absence of other mutagenic events. Recently, a novel gene termed MDM2, first identified in mouse as a small acentromeric extrachromosomal element, was shown to become oncogenic upon amplification and tumorigenic when overexpressed in NIH3T3 and Rat2 cells. The human homolog of MDM2 gene has been isolated and mapped to chromosome 12q13-q14, a region that is found to be amplified in human sarcomas. Sequence analysis of the murine, rat, and human MDM2 genes suggests that MDM2 may be involved in transcriptional regulation. MDM2 contains two putative metal-binding motifs, one of which shares similarity to known zinc finger transcriptional activators. MDM2 also contains a putative nuclear localization signal and an acidic domain of the type found in transcriptional activators. A functional role for MDM2 has recently been identified on the basis of experiments which demonstrate that MDM2 forms a stable complex with p53 in vivo. Furthermore, over-expression of MDM2 inhibits the ability of wild type p53 to stimulate expression of target genes and this inhibition appears to result from MDM2 binding to the acidic activation domain of p53, thereby preventing p53 from directly contacting the transcriptional machinery. Interestingly, wild type p53 appears to positively regulate expression of the MDM2 gene. MDM2 amplification has been observed in sarcomas and direct analysis of the MDM2 and p53 genes in sarcomas indicates that one or the other but not both of these genes is mutated in 70% of the tumors. Thus, it appears that genetic alterations in either p53 or MDM2 represent alternative mechanisms for inactivating the same growth suppressive pathway. Regulation of MDM2 expression by p53 also represents a potential feed back control of p53 function.
HostMouse
Immunogen speciesHuman
Immunogenrecombinant, human MDM2
Epitopewithin amino acids 153-222
Clone2A9C1.18
IsotypeIgG2a
Specieshuman, mouse
Positive controlA549 cells or most tissues
FormLiquid
FormulationIn 50 mM sodium phosphate buffer, 50% glycerol.
Concentration Label Please refer to vial label for lot-specific concentration
PreservativeNone
CommentsAntibody should be titrated for optimal results in individual systems.
Storage Avoid freeze/thaw
-20°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Toxicity Standard Handling
ReferencesMarchetti, A., et al. 1995. J. Pathol. 175, 31.
Barak, Y., et al. 1993. EMBO. J. 12, 461.
Ladanyi, M., et al. 1993. Cancer Res. 53, 16.
Leach, F. S., et al. 1993. Cancer Res. 53, 2231.
Oliner, J.D., et al. 1993. Nature 362, 857.
Momand, J., et al. 1992. Cell 69, 1237.
Oliner, J.D., et al. 1992. Nature 358, 80.
Fakharzadeh, S.S., et al. 1991. EMBO J. 10, 1565.