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MABD399 Anti-Lgr5/GPR49 Antibody, clone 5C8

Anti-Lgr5/GPR49 Antibody, clone 5C8 is an antibody against Lgr5/GPR49 for use in Flow Cytometry, Immunocytochemistry.

Anti-Lgr5/GPR49 Antibody, clone 5C8 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Stem Cell Research Gene Symbol: LGR5, GPR49, GPR67, GRP49, HG38 UniProt Number: O75473
MABD399
100 μg  
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Tabulka spec. kláve

Species ReactivityKey ApplicationsHostFormatAntibody Type
HFC, ICCRPurifiedMonoclonal Antibody
Description
Catalogue NumberMABD399
DescriptionAnti-Lgr5/GPR49 Antibody, clone 5C8
Alternate Names
  • Leucine-rich repeat-containing G-protein coupled receptor 5
  • G-protein coupled receptor 49
  • G-protein coupled receptor 67
  • G-protein coupled receptor HG38
  • Orphan G protein-coupled receptor HG38
Background InformationLeucine-rich repeat-containing G-protein coupled receptor 5 (UniProt O75473; also known as G-protein coupled receptor 49, G-protein coupled receptor 67, G-protein coupled receptor HG38, Orphan G protein-coupled receptor HG38) is encoded by the LGR5 (also known as GPR49, GPR67, GRP49, HG38) gene (Gene ID 8549) in human. Lgr5 is a 7-transmembrane protein related to the receptors for the follicle stimulating hormone (FSH), the luteinizing hormone (LH), and the thyroid-stimulating hormone (TSH). Lgr5 is a Wnt signaling pathway targeted gene expressed in proliferative stem cells in various tissues, including small intestine, colon, stomach, and the hair follicle. The R-spondin (RSPO) family of secreted Wnt signaling agonists are known to bind Lgr5 and the related Lgr4 and Lgr6, and RSPO1 depends on its association wth Lgr4, Lgr5, or Lgr6 for its Wnt signaling enhancing effect. Human Lgr5 is synthesized with a propeptide sequence (a.a. 1-21), the removal of which yields the mature 7-transmembrane (a.a. 562-582 , 594-614, 639-659, 683-703, 723-743, 768-788, 803-823) G-protein-coupled receptor (GPCR) having its N-terminal end (a.a. 22-561) at the extracellular side and its C-terminal end (a.a. 824-907) at the cytoplasmic side. The N-terminal extracellular region contains a leucine-rich repeat (LRR) N-terminal (LRRNT) domain (a.a. 25-66), followed by 16 LRRs (LRR 1 through LLR 16; a.a. 67-446).
References
Product Information
FormatPurified
PresentationPurified rat monoclonal IgG2bκ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationAnti-Lgr5/GPR49 Antibody, clone 5C8 is an antibody against Lgr5/GPR49 for use in Flow Cytometry, Immunocytochemistry.
Key Applications
  • Flow Cytometry
  • Immunocytochemistry
Application NotesImmunocytochemistry Analysis: 2.0 µg/mL of this antibody detected both transiently and stably expressed human Lgr5 in HEK293T cells (Courtesy of Dr. Wim de Lau, Hubrecht Institute and University Medical Center Utrecht, The Netherlands).
Biological Information
ImmunogenRecombinant protein corresponding to human Lgr5/GPR49.
Clone5C8
ConcentrationPlease refer to lot specific datasheet.
HostRat
IsotypeIgG2bκ
Species Reactivity
  • Human
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • LGR5
  • GPR49
  • GPR67
  • GRP49
  • HG38
Purification MethodProtein G Purified
UniProt Number
Molecular Weight~100 kDa calculated
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Flow Cytometry in human Lgr5-expressing stable LS174 (clone 11) cells.

Flow Cytometry Analysis: 0.1 µg of this antibody detected Lgr5/GPR49 in human Lgr5-expressing stable LS174 (clone 11) cells.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Katalogové číslo GTIN
MABD399 04055977297362