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06-1082 Anti-Interleukin 27 (IL-27) Antibody

Anti-Interleukin 27 (IL-27) Antibody detects level of Interleukin 27 (IL-27) & has been published & validated for use in WB, FC.

Anti-Interleukin 27 (IL-27) Antibody MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Inflammation & Immunology Research Sub-Category: Cytokines & Cytokine Receptors Gene Symbol: IL27A, IL27 UniProt Number: Q8NEV9
06-1082
100 µg  
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Species ReactivityKey ApplicationsHostFormatAntibody Type
H, ChpWB, FCRbAffinity PurifiedPolyclonal Antibody
Description
Catalogue Number06-1082
DescriptionAnti-Interleukin 27 (IL-27) Antibody
Alternate Names
  • Interleukin-27 subunit alpha
  • IL-27 subunit alpha
Background InformationInterleukin 27 (IL-27) is a heterodimeric cytokine belonging to the IL-12 family that is composed of two subunits: Epstein-Barr virus (EBV)-induced gene 3 (EBI3) (also known as IL-27B) and IL27-p28 (known as IL-27A). IL-27 has pro- and anti-inflammatory properties, that can regulate T helper cell development, suppress T-cell proliferation, stimulate cytotoxic T cell activity, induce isotype switching in B-cells, and that has diverse effects on innate immune cells. IL-27 is secreted by antigen-presenting cells and plays an important function in regulating the activity of B and T lymphocytes. IL-27 suppresses the expression of GATA3, the inhibitor TH1 cells development. In addition to suppressing cytokine production, IL-27 also antagonizes the effects of some cytokines such as IL6 through direct effects on T cells. Another important role of IL-27 is its antitumor activity as well as its antiangiogenic activity with activation of production of antiangiogenic chemokines such as IP-10/CXCL10 and MIG/CXCL9. In vein endothelial cells, it induces IRF1/interferon regulatory factor 1 and increase the expression of MHC class II transactivator/CIITA with resulting up-regulation of major histocompatibility complex class II. IL-27 also demonstrates antiviral activity with inhibitory properties on HIV-1 replication (Uniprot/Swissprot).
References
Product Information
FormatAffinity Purified
Control
  • HeLa cell lysate
PresentationPurified rabbit polyclonal in buffer containing PBS with 0.08% sodium azide.
Applications
ApplicationAnti-Interleukin 27 (IL-27) Antibody detects level of Interleukin 27 (IL-27) & has been published & validated for use in WB, FC.
Key Applications
  • Western Blotting
  • Flow Cytometry
Application NotesFlow Cytometry Analysis: 5 µg from a previous lot was used to detect IL-27 in human PBMCs.
Biological Information
ImmunogenKLH-conjugated linear peptide corresponding to the mature chain of Interleukin 27 (IL-27), subunit alpha.
EpitopeMature chain of IL-27, subunit alpha.
ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
HostRabbit
SpecificityThis antibody recognizes the mature chain of interleukin 27 (IL-27), subunit alpha.
In endogenous lysates, antibody may detect the heterodimeric IL-27, composed of both IL-27 subunit alpha and beta.
Species Reactivity
  • Human
  • Chimpanzee
Species Reactivity NoteDemonstrated to react with human.
Predicted to react with Chimpanzee based on 100% sequence homology.
80% (12/15 amino acids) rat and mouse.
Antibody TypePolyclonal Antibody
Entrez Gene Number
Entrez Gene SummaryThe protein encoded by this gene is one of the subunits of a heterodimeric cytokine complex. This protein is related to interleukin 12A (IL12A). It interacts with Epstein-Barr virus induced gene 3 (EBI3), a protein similar to interleukin 12B (IL12B), and forms a complex that has been shown to drive rapid expansion of naive but not memory CD4(+) T cells. The complex is also found to synergize strongly with interleukin 12 to trigger interferon gamma (IFNG) production of naive CD4(+) T cells. The biological effects of this cytokine are mediated by the class I cytokine receptor (WSX1/TCRR). [provided by RefSeq]
Gene Symbol
  • IL27A
  • IL27
Purification MethodAffinity Purfied
UniProt Number
UniProt SummaryFUNCTION: Cytokine with pro- and anti-inflammatory properties, that can regulate T helper cell development, suppress T-cell proliferation, stimulate cytotoxic T cell activity, induce isotype switching in B-cells, and that has diverse effects on innate immune cells. Among its target cells are CD4 T helper cells which can differentiate in type 1 effector cells (TH1), type 2 effector cells (TH2) and IL17 producing helper T-cells (TH17). It drives rapid clonal expansion of naive but not memory CD4 T-cells. It also strongly synergizes with IL-12 to trigger interferon-gamma/IFN-gamma production of naive CD4 T-cells, binds to the cytokine receptor WSX-1/TCCR which appears to be required but not sufficient for IL-27-mediated signal transduction. IL-27 potentiate the early phase of TH1 response and suppress TH2 and TH17 differentiation. It induces the differentiation of TH1 cells via two distinct pathways, p38 MAPK/TBX21- and ICAM1/ITGAL/ERK-dependent pathways. It also induces STAT1, STAT3, STAT4 and STAT5 phosphorylation and activates TBX21/T-Bet via STAT1 with resulting IL12RB2 up-regulation, an event crucial to TH1 cell commitment. It suppresses the expression of GATA3, the inhibitor TH1 cells development. In CD8 T-cells, it activates STATs as well as GZMB. IL-27 reveals to be a potent inhibitor of TH17 cell development and of IL-17 production. Indeed IL-27 subunit p28 alone is also able to inhibit the production of IL17 by CD4 and CD8 T-cells. While IL-27 suppressed the development of proinflammatory Th17 cells via STAT1, it inhibits the development of anti-inflammatory inducible regulatory T-cells, iTreg, independently of STAT1. IL-27 has also an effect on cytokine production, it suppresses proinflammatory cytokine production such as IL2, IL4, IL5 and IL6 and activates suppressors of cytokine signaling such as SOCS1 and SOCS3. Apart from suppression of cytokine production, IL-27 also antagonizes the effects of some cytokines such as IL6 through direct effects on T cells. Another important role of IL-27 is its antitumor activity as well as its antiangiogenic activity with activation of production of antiangiogenic chemokines such as IP-10/CXCL10 and MIG/CXCL9. In vein endothelial cells, it induces IRF1/interferon regulatory factor 1 and increase the expression of MHC class II transactivator/CIITA with resulting up-regulation of major histocompatibility complex class II. IL-27 also demonstrates antiviral activity with inhibitory properties on HIV-1 replivation.
SUBUNIT STRUCTURE: Heterodimer with IL27B/EBI3. IL-27 is an heterodimer composed IL27 and EBI3. EBI3 is also a component of the IL-12 heterodimer.
SUBCELLULAR LOCATION: Secreted. Note: Does not seem to be secreted without coexpression of EBI3.
TISSUE SPECIFICITY: Expressed in monocytes and in placenta.
INDUCTION: Transiently induced by LPS stimulation in monocytes.
POST-TRANSLATIONAL MODIFICATION: O-glycosylated.
SEQUENCE SIMILARITIES: Belongs to the IL-6 superfamily.
Molecular WeightCalculated molecular weight ~27 kDa.
In Western Blot, this antibody detects a band at ~40 kDa in HeLa lysate corresonding to the heterodimeric IL-27 composed of IL-27A and IL-27B.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blot in HeLa cell lysates.

Western Blot Analysis: 1 µg/mL of this antibody was used to detect IL-27 in HeLa cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 µg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Katalogové číslo GTIN
06-1082 04053252732751