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MAB1956Z Anti-Integrin α5 Antibody, clone P1D6, azide free

MAB1956Z
100 µg  
Purchase on Sigma-Aldrich

Speciální nabídky

Přehled

Speciální nabídky

Tabulka spec. kláve

Species ReactivityKey ApplicationsHostFormatAntibody Type
HIHC, FUNCMPurifiedMonoclonal Antibody
Description
Catalogue NumberMAB1956Z
Brand Family Chemicon®
Trade Name
  • Chemicon
DescriptionAnti-Integrin α5 Antibody, clone P1D6, azide free
Alternate Names
  • CD49e
  • MAB1956
Background InformationIntegrin alpha 5 belongs to the integrin alpha chain family. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. This gene encodes the integrin alpha 5 chain. Alpha chain 5 undergoes post translational cleavage in the extracellular domain to yield disulfide linked light and heavy chains that join with beta 1 to form a fibronectin receptor. In addition to adhesion, integrins are known to participate in cell surface mediated signalling.
Product Information
FormatPurified
Control
  • Widely expressed, ovary
PresentationPurified mouse monoclonal in 0.01M Phosphate, 150mM NaCl, pH 7.4, containing no preservatives.
Quality LevelMQ100
Applications
ApplicationThis Anti-Integrin α5 Antibody, clone P1D6, azide free is validated for use in IH, FUNC for the detection of Integrin α5.
Key Applications
  • Immunohistochemistry
  • Affects Function
Application NotesMAB1956Z is suitable for use in attachment inhibition assays using fibroblasts, endothelial cells, platelets and T & B lymphocytes on fibronectin.

MAB1956Z is not reactive with the denatured alpha 5 subunit. For immunoblotting, a dot blot is suggested as an alternative to western blotting.
Biological Information
CloneP1D6
ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
HostMouse
SpecificityReacts with Human alpha 5 integrin. Reactivity with other species is unknown.
IsotypeIgG3
Species Reactivity
  • Human
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Entrez Gene SummaryThe product of this gene belongs to the integrin alpha chain family. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. This gene encodes the integrin alpha 5 chain. Alpha chain 5 undergoes post-translational cleavage in the extracellular domain to yield disulfide-linked light and heavy chains that join with beta 1 to form a fibronectin receptor. In addition to adhesion, integrins are known to participate in cell-surface mediated signalling.
Gene Symbol
  • ITGA5
  • VLA-5
  • CD49e
  • VLA5A
  • FNRA
Purification MethodProtein A Purfied
UniProt Number
UniProt SummaryFUNCTION: SwissProt: P08648 # Integrin alpha-5/beta-1 is a receptor for fibronectin and fibrinogen. It recognizes the sequence R-G-D in its ligands. In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions.
SIZE: 1049 amino acids; 114536 Da
SUBUNIT: Heterodimer of an alpha and a beta subunit. The alpha subunit is composed of an heavy and a light chain linked by a disulfide bond. Alpha-5 associates with beta-1. Interacts with HPS5. Interacts with HIV-1 Tat.
SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein.
SIMILARITY: SwissProt: P08648 ## Belongs to the integrin alpha chain family. & Contains 7 FG-GAP repeats.
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsMaintain for 1 year at 2–8°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Packaging Information
Material Size100 µg
Global Trade Item Number
Katalogové číslo GTIN
MAB1956Z 04053252610004