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04-1664 Anti-Histidine Tag Antibody, clone RM146

This Anti-Histidine Tag Antibody, clone RM146 is validated for use in Western Blotting, Immunoprecipitation for the detection of Histidine Tag.

Anti-Histidine Tag Antibody, clone RM146 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Secondary & Control Antibodies Research Sub-Category: Secondary Antibodies Adsorbed for Dual Labeling
04-1664
100 μg  
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Speciální nabídky

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Tabulka spec. kláve

Species ReactivityKey ApplicationsHostFormatAntibody Type
A Broad Range Of SpeciesWB, IPRbPurifiedMonoclonal Antibody
Description
Catalogue Number04-1664
DescriptionAnti-Histidine Tag Antibody, clone RM146
References
Product Information
FormatPurified
PresentationRabbit monoclonal in PBS with 1% BSA and 0.09% sodium azide.
Quality LevelMQ100
Applications
ApplicationThis Anti-Histidine Tag Antibody, clone RM146 is validated for use in Western Blotting, Immunoprecipitation for the detection of Histidine Tag.
Key Applications
  • Western Blotting
  • Immunoprecipitation
Application NotesImmunoprecipitation Analysis (IP): 0.5-2 μg/mL from a representative lot detected Histidine Tag in HEK293T whole lysate control and IP by clone RM146.
Biological Information
ImmunogenMixture of a peptide with 6xHis-Tag at the N-terminus and another peptide with 6xHis-Tag at the C-terminus.
CloneRM146
ConcentrationPlease refer to lot specific datasheet.
HostRabbit
SpecificityThis antibody reacts to recombinant proteins containing the 6xHis-Tag or 10xHis-Tag fused to either the amino or carboxy terminus. No cross reactivity with other endogenous protein in mammalian or bacteria cells.
IsotypeIgG
Species Reactivity
  • A Broad Range Of Species
Antibody TypeMonoclonal Antibody
Purification MethodProtein A Purfied
Molecular Weight~52 and ~85 kDa observed. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in HEK293T cell lysate untransfected with His-Tag fusion protein X, transfected with His-Tag fusion protein X, E. coli lysate without His-Tag Protein Y, and E. coli lysate with His-Tag Protein Y.

Western Blotting Analysis (WB): 0.2 μg/mL of this antibody detected Histidine Tag in HEK293T cell lysate transfected with His-Tag fusion protein X and E. coli lysate with His-Tag Protein Y.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
Packaging Information
Material Size100 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Katalogové číslo GTIN
04-1664 04055977181333

Documentation

Anti-Histidine Tag Antibody, clone RM146 MSDS

Title

Safety Data Sheet (SDS) 

Anti-Histidine Tag Antibody, clone RM146 Certificates of Analysis

TitleLot Number
Anti-Histidine Tag, clone RM146 - VP1908022 VP1908022
Anti-Histidine Tag, clone RM146 - VP1911081 VP1911081
Anti-Histidine Tag, clone RM146 - VP2108263 VP2108263
Anti-Histidine Tag, clone RM146 - VP2112173 VP2112173
Anti-Histidine Tag, clone RM146 - VP2202182 VP2202182
Anti-Histidine Tag, clone RM146 - VP2205198 VP2205198
Anti-Histidine Tag, clone RM146 - VP2303021 VP2303021
Anti-Histidine Tag, clone RM146 - VP2309134 VP2309134
Anti-Histidine Tag, clone RM146 - VP2405022 VP2405022
Anti-Histidine Tag, clone RM146 -VP1511216 VP1511216
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Technical Info

Title
Characterization of Estrogen Receptor α Phosphorylation Sites in Breast Cancer Tissue Using the SNAP i.d® 2.0 System
White Paper: Further considerations of antibody validation and usage.