Millipore Sigma Vibrant Logo
Attention: We have moved. Merck Millipore products are no longer available for purchase on MerckMillipore.com.Learn More

MABE337 Anti-Hexim 1 Antibody, clone 10D2.1

This Anti-Hexim 1 Antibody, clone 10D2.1 is validated for use in Western Blotting and Immunohistochemistry (Paraffin) for the detection of Hexim 1.

Anti-Hexim 1 Antibody, clone 10D2.1 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Epigenetics & Nuclear Function Research Sub-Category: Nuclear Receptors Gene Symbol: HEXIM1, CLP1, EDG1, HIS1, MAQ1 UniProt Number: O94992
MABE337
200 µg  
Purchase on Sigma-Aldrich

Speciální nabídky

Přehled

Replacement Information

Speciální nabídky

100% Performance Guaranteed

Tabulka spec. kláve

Species ReactivityKey ApplicationsHostFormatAntibody Type
HWB, IH(P)MPurifiedMonoclonal Antibody
Description
Catalogue NumberMABE337
DescriptionAnti-Hexim 1 Antibody, clone 10D2.1
Alternate Names
  • Protein HEXIM1
  • Cardiac lineage protein 1
  • Estrogen down-regulated gene 1 protein
  • Hexamethylene bis-acetamide-inducible protein 1
  • Menage a quatre protein 1
Background InformationHexim1 is a transcriptional regulator which functions as a general RNA polymerase II transcription inhibitor. Hexim1 works in cooperation with 7SK snRNA to sequester P-TEFb in a large inactive 7SK snRNP complex, preventing RNA polymerase II phosphorylation and subsequent transcriptional elongation. Hexim1 may also regulate NF-kappa-B, ESR1, NR3C1 and CIITA-dependent transcriptional activity. The 7SK snRNP complex is composed of P-TEFb (composed of CDK9 and CCNT1/cyclin-T1), Hexim1, Hexim2, BCDIN3, SART3 proteins and 7SK and U6 snRNAs. Hexim1 also interacts with the N-CoR complex through NCOR1, ESR1, NR3C1, and may interact with NF-kappa-B through REL A. Hexim1 is located in the nucleus where it can bind with alpha-importin, and can also be found in the cytoplasm. Hexim1 is up-regulated by HMBA (hexamethylene bisacetamide) at the protein level and down-regulated by estrogen.
References
Product Information
FormatPurified
PresentationPurified mouse monoclonal IgG2bκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationThis Anti-Hexim 1 Antibody, clone 10D2.1 is validated for use in Western Blotting and Immunohistochemistry (Paraffin) for the detection of Hexim 1.
Key Applications
  • Western Blotting
  • Immunohistochemistry (Paraffin)
Application NotesImmunohistochemistry Analysis: A 1:50-250 dilution from a representative lot detected Hexim 1 in human placenta tissue.
Biological Information
ImmunogenGST-tagged recombinant protein corresponding to the C-terminus of human Hexim 1.
EpitopeC-terminus
Clone10D2.1
ConcentrationPlease refer to lot specific datasheet.
HostMouse
IsotypeIgG2bκ
Species Reactivity
  • Human
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • HEXIM1
  • CLP1
  • EDG1
  • HIS1
  • MAQ1
Purification MethodProtein G Purified
UniProt Number
Molecular Weight~60 kDa observed
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in human placenta tissue lysate.

Western Blotting Analysis: 2.0 µg/mL of this antibody detected Hexim 1 in 10 µg of human placenta tissue lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size200 µg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Katalogové číslo GTIN
MABE337 04055977073652