Millipore Sigma Vibrant Logo
Attention: We have moved. Merck Millipore products are no longer available for purchase on MerckMillipore.com.Learn More

ABD113-25UG Anti-HLA-DRB3

Detect HLA-DRB3 using this rabbit polyclonal Anti-HLA-DRB3 Antibody, Cat. No. ABD113-25UG, validated for use in Western Blotting and Flow Cytometry.

Anti-HLA-DRB3 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Apoptosis & Cancer Gene Symbol: HLA-DRB3 UniProt Number: P79483
ABD113-25UG
25 μg  
Purchase on Sigma-Aldrich

Přehled

Replacement Information

Tabulka spec. kláve

Species ReactivityKey ApplicationsHostFormatAntibody Type
HWB, FCRbAffinity PurifiedPolyclonal Antibody
Description
Catalogue NumberABD113-25UG
DescriptionAnti-HLA-DRB3
Alternate Names
  • HLA class II histocompatibility antigen
  • DR beta 3 chain
  • MHC class II antigen DRB3
Background InformationHLA class II histocompatibility antigen, DR beta 3 chain (UniProt: P79483; also known as MHC class II antigen DRB3) is encoded by the HLA-DRB3 gene (Gene ID: 3125) in human. DRB3 is a single-pass type I membrane protein; that is synthesized with a signal peptide of 29 amino acids that is subsequently cleaved to generate a mature form. It also contains an Ig0like C1 type domain (aa 126-214). It is a heterodimer of an alpha and a beta subunit; also referred as MHC class II molecule. To produce a MHC class II molecule that presents an antigen, three MHC class II molecules (heterodimers of an alpha and a beta chain) associate with a CD74 trimer in the endoplasmic reticulum (ER) to form a heterononamer. The MHC class II complex transits through a number of intracellular compartments in the endocytic pathway until it reaches the cell membrane for antigen presentation. DRB3 binds peptides derived from antigens that access the endocytic route of antigen presenting cells (APC) and presents them on the cell surface for recognition by the CD4 T-cells.
References
Product Information
FormatAffinity Purified
PresentationPurified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationDetect HLA-DRB3 using this rabbit polyclonal Anti-HLA-DRB3 Antibody, Cat. No. ABD113-25UG, validated for use in Western Blotting and Flow Cytometry.
Key Applications
  • Western Blotting
  • Flow Cytometry
Application NotesFlow Cytometry Analysis: 1 µg from a representative lot detected HLA-DRB3 in one million Raji cells.
Biological Information
ImmunogenKLH-conjugated linear peptide corresponding to 15 amino acids from the N-terminal half of human HLA class II histocompatibility antigen, DR beta 3 chain (HLA-DRB3).
ConcentrationPlease refer to lot specific datasheet.
HostRabbit
SpecificityThis rabbit polyclonal antibody detects HLA class II histocompatibility antigen, DR beta 3 chain in human cells. It targets an epitope within 15 amino acids from the N-terminal half.
IsotypeIgG
Species Reactivity
  • Human
Species Reactivity NoteHuman.
Antibody TypePolyclonal Antibody
Gene Symbol
  • HLA-DRB3
Purification MethodAffinity Purified
UniProt Number
Molecular Weight32 kDa kDa observed. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in Raji cell lysate.
Western Blotting Analysis: 2 µg/mL of this antibody detected HLA-DRB3 in 10 µg of Raji cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size25 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Katalogové číslo GTIN
ABD113-25UG 04054839453762