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MABC1149 Anti-HAF/SART1 Antibody, clone 1-2

Detect HAF/SART1 using this mouse monoclonal Anti-HAF/SART1, clone 1-2 Antibody, Cat. No. MABC1149, validated for use in Western Blotting and Immunocytochemistry, Immunohistochemistry, and Western Blotting.

Anti-HAF/SART1 Antibody, clone 1-2 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Apoptosis & Cancer Gene Symbol: SART1 UniProt Number: O43290
MABC1149
100 µL  
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Tabulka spec. kláve

Species ReactivityKey ApplicationsHostFormatAntibody Type
HWB, ICC, IHCMPurifiedMonoclonal Antibody
Description
Catalogue NumberMABC1149
DescriptionAnti-HAF/SART1 Antibody, clone 1-2
Alternate Names
  • U4/U6.U5 tri-snRNP-associated protein 1
  • Allergen Hom s 1
  • HAF
  • hSART-1
  • hSnu66
  • SART-1
  • Hypoxia-associated factor
  • SNU66 homolog
  • Squamous cell carcinoma antigen recognized by T-cells 1
  • U4/U6.U5 tri-snRNP-associated 110 kDa protein
Background InformationU4/U6.U5 tri-snRNP-associated protein 1 (UniProt O43290; also known as Allergen Hom s 1, HAF, hSART-1, hSnu66, Hypoxia-associated factor, SART-1, SNU66 homolog, Squamous cell carcinoma antigen recognized by T-cells 1, U4/U6.U5 tri-snRNP-associated 110 kDa protein) is encoded by the SART1 (also known as HAF) gene (Gene ID 9092) in human. The hypoxia-associated factor (HAF) is an E3 ubiquitin ligase that mediates the ubiquitination and degradation of hypoxia-inducible factor 1α (HIF-1α). HAF also binds to HIF-2α and promotes HIF-2 transactivation instead of promoting HIF-2α degradation. In addition, hypoxia-dependent HAF SUMOylation enhances HAF interaction with HIF-2α and induction of HIF-2 target genes transcription, such as OCT-3/4, SOX2 and NANOG. HAF therefore promotes stem cell characteristics, aggressive tumor growth and invasion by switching cells from HIF-1α- to HIF-2α-dependent signaling. HAF overexpression enhances glioblastoma cells that express both HIF-1α and HIF-2α to initiate tumors as intracranial xenografts in mice. On the other hand, HAF overexpression results in decreased xenograft tumor growth of HT29 colon carcinoma cells that only express HIF-1α.
References
Product Information
FormatPurified
PresentationPurified mouse IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide
Quality LevelMQ100
Applications
ApplicationDetect HAF/SART1 using this mouse monoclonal Anti-HAF/SART1, clone 1-2 Antibody, Cat. No. MABC1149, validated for use in Western Blotting and Immunocytochemistry, Immunohistochemistry, and Western Blotting.
Key Applications
  • Western Blotting
  • Immunocytochemistry
  • Immunohistochemistry
Application NotesImmunocytochemistry Analysis: A 1:200 dilution from a representative lot detected HAF/SART1 in A431 cells.

Western Blotting Analysis: A 1:62.5 dilution from a representative lot detected HAF/SART1 in 10 µg of Jurkat cell lysate.

Immunocytochemistry Analysis: A representative lot detected a reduced HAF immunoreactivity in MIA PaCa-2 human pancreatic carcinoma cells following HAF shRNA treatment (Courtesy of Mei Yee Koh, Ph.D., Sanford-Burnham Medical Research Institute, La Jolla, CA USA).

Immunocytochemistry Analysis: A representative lot detected HAF in HeLa and PANC-1 cells (Courtesy of Mei Yee Koh, Ph.D., Sanford-Burnham Medical Research Institute, La Jolla, CA USA).

Immunocytochemistry Analysis: A representative lot detected an increased HAF immunoreactivity in 786-O human kidney cancer cells transfected to overexpress HAF (Courtesy of Mei Yee Koh, Ph.D., Sanford-Burnham Medical Research Institute, La Jolla, CA USA).

Immunohistochemistry Analysis: A representative lot detected HAF immunoreactivity in 786-O xenograft (Courtesy of Mei Yee Koh, Ph.D., Sanford-Burnham Medical Research Institute, La Jolla, CA USA).
Biological Information
ImmunogenGST-tagged recombinant human HAF/SART1 C-terminal fragment.
Clone1-2
ConcentrationPlease refer to lot specific datasheet.
HostMouse
SpecificityClone 1-2 detected HAF shRNA-mediated HAF knockdown in MIA PaCa-2 human pancreatic carcinoma cells.
IsotypeIgG1κ
Species Reactivity
  • Human
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • SART1
Purification MethodProtein G purified.
UniProt Number
Molecular Weight~110 kDa observed. 90.26 kDa calculated. The larger-than-calculated target band size is consistent with that reported in the literature (Koh, M.Y. et al. (2015). Cancer Res. 75(2):316-329). Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in HeLa cell lysate.

Western Blotting Analysis: A 1:62.5 dilution from a representative lot detected HAF/SART1 in 10 µg of HeLa cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Katalogové číslo GTIN
MABC1149 04054839083730