Millipore Sigma Vibrant Logo
Attention: We have moved. Merck Millipore products are no longer available for purchase on MerckMillipore.com.Learn More

PK1111 Anti-GSK-3β Rabbit pAb

Anti-GSK-3β, rabbit polyclonal, recognizes the ~46 kDa GSK-3β protein in mouse small intestine tissue. It is validated for use in Western blotting and immunohistochemistry.

Anti-GSK-3β Rabbit pAb MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
PK1111
  
Purchase on Sigma-Aldrich

Přehled

Replacement Information

Tabulka spec. kláve

Species ReactivityHostAntibody Type
H, M, RRbPolyclonal Antibody
Description
Overview

This product has been discontinued.



small intestine tissue.
Catalogue NumberPK1111
Brand Family Calbiochem®
Application Data
Detection of mouse GSK-3β by immunoblotting. Sample: Whole tissue extract from mouse small intestine. Primary antibody: Anti-GSK-3β Rabbit pAb (Cat. No. PK1111). Detection: chemiluminescence.
References
ReferencesGarrido, J.J., et al. 2007.FEBS Lett. 8, 1579.
Meares, G.P. and Jope, R.S. 2007.J Biochem. 23, 16989.
Product Information
FormLiquid
FormulationIn PBS, 30% glycerol, 0.5% BSA.
Positive controlMouse small intestine
Preservative0.01% thimerosal
Quality LevelMQ100
Applications
Key Applications Immunoblotting (Western Blotting)
Immunohistochemistry
Application NotesImmunoblotting (0.5-4 µg/ml)
Immunohistochemistry (10-30 µg/ml; see comments)
Application CommentsThis antibody has been reported to work for immunohistochemistry in the range of 10-30 µg/ml, but the optimal concentration should be determined for each experiment. Antibody should be titrated for optimal results in individual systems.
Biological Information
Immunogenrecombinant human GSK-3β
ImmunogenHuman
HostRabbit
IsotypeIgG
Species Reactivity
  • Human
  • Mouse
  • Rat
Antibody TypePolyclonal Antibody
Concentration Label Please refer to vial label for lot-specific concentration
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Blue Ice Only
Toxicity Standard Handling
Storage -20°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C) for long-term storage.
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Katalogové číslo GTIN
PK1111 0

Documentation

Anti-GSK-3β Rabbit pAb MSDS

Title

Safety Data Sheet (SDS) 

Anti-GSK-3β Rabbit pAb Certificates of Analysis

TitleLot Number
PK1111

References

Přehled odkazů
Garrido, J.J., et al. 2007.FEBS Lett. 8, 1579.
Meares, G.P. and Jope, R.S. 2007.J Biochem. 23, 16989.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision16-September-2016 JSW
ApplicationImmunoblotting (0.5-4 µg/ml)
Immunohistochemistry (10-30 µg/ml; see comments)
Application Data
Detection of mouse GSK-3β by immunoblotting. Sample: Whole tissue extract from mouse small intestine. Primary antibody: Anti-GSK-3β Rabbit pAb (Cat. No. PK1111). Detection: chemiluminescence.
DescriptionImmunoaffinity purified rabbit polyclonal antibody. Recognizes the ~46 kDa GSK-3 protein.
BackgroundGlycogen synthase kinase 3 (GSK-3) is a serine/threonine protein kinase that plays a role in the regulation of cell survival and in the Wnt signaling pathway. Its activity can be inhibited by Akt-mediated phosphorylation at Ser21 in GSK-3α and Ser9 in GSK-3β. GSK-3 is also known to phosphorylate the six isoforms of Tau, indicating a link to Alzheimer's disease.
HostRabbit
Immunogen speciesHuman
Immunogenrecombinant human GSK-3β
IsotypeIgG
Specieshuman, mouse, rat
Positive controlMouse small intestine
FormLiquid
FormulationIn PBS, 30% glycerol, 0.5% BSA.
Concentration Label Please refer to vial label for lot-specific concentration
Preservative0.01% thimerosal
CommentsThis antibody has been reported to work for immunohistochemistry in the range of 10-30 µg/ml, but the optimal concentration should be determined for each experiment. Antibody should be titrated for optimal results in individual systems.
Storage Avoid freeze/thaw
-20°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C) for long-term storage.
Toxicity Standard Handling
ReferencesGarrido, J.J., et al. 2007.FEBS Lett. 8, 1579.
Meares, G.P. and Jope, R.S. 2007.J Biochem. 23, 16989.