Millipore Sigma Vibrant Logo
Attention: We have moved. Merck Millipore products are no longer available for purchase on MerckMillipore.com.Learn More

CB1031 Anti-GHRL Mouse mAb (2F4)

Anti-GHRL Mouse mAb (2F4) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Anti-Ghrelin

CB1031
  
Purchase on Sigma-Aldrich

Přehled

Replacement Information

Tabulka spec. kláve

Host
M
Description
Overview

This product has been discontinued.





Recognizes the ~12 kDa GHRL protein in HeLa cells and human stomach tissue.
Catalogue NumberCB1031
Brand Family Calbiochem®
SynonymsAnti-Ghrelin
Application Data
Detection of human GHRL by immunoblotting. Sample: Whole cell lysate from HeLa cells. Primary antibody: Anti-GHRL Mouse mAb (2F4) (Cat. No. CB1031) (1-5 µg/ml). Detection: chemiluminescence.

Detection of human GHRL by staining paraffin sections. Sample: Formalin-fixed, paraffin-embedded human stomach tissue. Primary antibody: Anti-GHRL Mouse mAb (2F4) (Cat. No. CB1031) (3 µg/ml). Detection: colorimetric (DAB).
References
Product Information
Formliquid
FormulationIn PBS, pH 7.2.
Positive controlHeLa cells, Human stomach tissue
PreservativeNone
Applications
Key Applications Enzyme-Linked Immunosorbent Assay
Immunoblotting (Western Blotting)
Paraffin Sections
Application NotesELISA (see comments)
Immunoblotting (1-5 µg/ml)
Paraffin Sections (3 µg/ml))
Application CommentsFor ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
Biological Information
ImmunogenFull-length, recombinant human rGHRL (aa 1-118), expressed as a GST fusion protein
ImmunogenNA
Clone2F4
HostMouse
IsotypeIgG2a
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Blue Ice Only
Toxicity Regulatory Review
Storage -20°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Katalogové číslo GTIN
CB1031 0

Documentation

Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision29-October-2010 RFH
SynonymsAnti-Ghrelin
ApplicationELISA (see comments)
Immunoblotting (1-5 µg/ml)
Paraffin Sections (3 µg/ml))
Application Data
Detection of human GHRL by immunoblotting. Sample: Whole cell lysate from HeLa cells. Primary antibody: Anti-GHRL Mouse mAb (2F4) (Cat. No. CB1031) (1-5 µg/ml). Detection: chemiluminescence.

Detection of human GHRL by staining paraffin sections. Sample: Formalin-fixed, paraffin-embedded human stomach tissue. Primary antibody: Anti-GHRL Mouse mAb (2F4) (Cat. No. CB1031) (3 µg/ml). Detection: colorimetric (DAB).
DescriptionPurified mouse monoclonal antiboy. Recognizes the ~12 kDa GHRL protein.
BackgroundThis gene encodes ghrelin-obestatin preproprotein, which generates ghrelin and obestatin. Ghrelin is an endogenous ligand for the growth hormone secretagogue receptor and is involved in regulating growth hormone release. Obestatin was initially reported to be an endogenous ligand for the orphan G protein-coupled receptor GPR39 and was involved in satiety and decreased food intake; however, these findings are controversial. Recent reports show that obestatin is involved in inhibiting thirst and anxiety, improving memory, regulating sleep, affecting cell proliferation, and increasing the secretion of pancreatic juice enzymes.
HostMouse
Immunogen speciesNA
ImmunogenFull-length, recombinant human rGHRL (aa 1-118), expressed as a GST fusion protein
Clone2F4
IsotypeIgG2a
Specieshuman
Positive controlHeLa cells, Human stomach tissue
Formliquid
FormulationIn PBS, pH 7.2.
PreservativeNone
CommentsFor ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
Storage Avoid freeze/thaw
-20°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Toxicity Regulatory Review