Millipore Sigma Vibrant Logo
Attention: We have moved. Merck Millipore products are no longer available for purchase on MerckMillipore.com.Learn More

ABE476 Anti-GAL4 Antibody (activation domain)

ABE476
100 µL  
Purchase on Sigma-Aldrich

Speciální nabídky

Přehled

Replacement Information

Speciální nabídky

Tabulka spec. kláve

Species ReactivityKey ApplicationsHostFormatAntibody Type
YeastWBRbAffinity PurifiedPolyclonal Antibody
Description
Catalogue NumberABE476
Replaces06-283
DescriptionAnti-GAL4 Antibody (activation domain)
Alternate Names
  • Regulatory protein GAL4
Background InformationGAL4 is a transcriptional activator which controls the expression of genes involved in the catabolism of galactose. In addition to a DNA-binding domain, Gal4 contains two acidic regions: activating region I (aa148-238) and activating region II (aa768-881), which can both activate transcription when independently joined to the DNA-binding domain. The activation domains of Gal4 have been shown to interact with Gal80, TBP, SUG1, and ADA2, among other general transcription factors.
References
Product Information
FormatAffinity Purified
Control
  • Gal4 positive and Gal4 negative yeast
PresentationPurified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationUse Anti-GAL4 (activation domain) Antibody (Rabbit Polyclonal Antibody) validated in WB to detect GAL4 (activation domain) also known as Regulatory protein GAL4.
Key Applications
  • Western Blotting
Biological Information
ImmunogenGST-tagged recombinant protein corresponding to the C-terminus of yeast GAL4 (activation domain).
EpitopeC-terminus
HostRabbit
SpecificityThis antibody recognizes the C-terminus of GAL4 (activation domain).
Species Reactivity
  • Yeast
Antibody TypePolyclonal Antibody
Entrez Gene Number
Gene Symbol
  • GAL4
Purification MethodAffinity Purfied
UniProt Number
Molecular Weight~28 kDa, ~38 kDa, ~77 kDa observed
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blot in Gal4 positive and Gal4 negative yeast cell lysate.

Western Blot Analysis: A 1:160,000 dilution of this antibody detected Gal4 in 5 mg of Gal4 positive (+ recombinant Gal4) yeast cell lysate. This antibody also detected small mounts of endogenous Gal4 in Gal4 negative (- recombinant Gal4) yeast cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Katalogové číslo GTIN
ABE476 04053252522741

Documentation

Anti-GAL4 Antibody (activation domain) MSDS

Title

Safety Data Sheet (SDS) 

Anti-GAL4 Antibody (activation domain) Certificates of Analysis

TitleLot Number
Anti-GAL4 (activation domain) - 2394037 2394037
Anti-GAL4 (activation domain) - 2363046 2363046
Anti-GAL4 (activation domain) - 2857210 2857210
Anti-GAL4 (activation domain) - 3098934 3098934
Anti-GAL4 (activation domain) - 3385848 3385848
Anti-GAL4 (activation domain) - 3934340 3934340
Anti-GAL4 (activation domain) - Q2030507 Q2030507
Anti-GAL4 (activation domain) -2501496 2501496
Anti-GAL4 (activation domain) -2641700 2641700
Anti-GAL4 (activation domain) -2793791 2793791