Millipore Sigma Vibrant Logo
Attention: We have moved. Merck Millipore products are no longer available for purchase on MerckMillipore.com.Learn More

CP28 Anti-Fibroblast Antigen Mouse mAb (AS02)

Anti-Fibroblast Antigen, mouse monoclonal, clone AS02, detects ~30-31 kDa CD90/Thy-1 antigen in human fibroblasts (non-reducing conditions). It is validated for FC, WB, IF, IP & IHC (frozen sectio

 More>>
Anti-Fibroblast Antigen Mouse mAb (AS02) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Anti-CD90 (Ab-1) (Mouse), Anti-Thy-1 (Ab-1) (Mouse)

CP28
  
Purchase on Sigma-Aldrich

Přehled

Tabulka spec. kláve

Species ReactivityHostAntibody Type
H, MkMMonoclonal Antibody
Description
OverviewRecognizes the ~30-31 kDa CD90/Thy-1 antigen in human fibroblasts under non-reducing conditions.
Catalogue NumberCP28
Brand Family Calbiochem®
SynonymsAnti-CD90 (Ab-1) (Mouse), Anti-Thy-1 (Ab-1) (Mouse)
References
ReferencesSaalbach, A., et al. 1996 J. Invest. Dermatol. 106, 1314.
Janin, A., et al. 1990. Clin. Exp. Rheum. 8, 237.
Kay, M., et al. 1989. J. Invest. Dermatol. 92, 168.
Esterre, P.H., et al. 1984 Cell Mol. Biol. 38, 297.
Hoyhtya, M., et al. 1984 Eur. J. Biochem 141, 477.
Product Information
DeclarationManufactured by Dianova, GmbH, Germany.
FormLiquid
FormulationIn 50 mM sodium phosphate buffer, 6% BSA.
Positive controlAny fibroblast cell line or skin tissue
Preservative≤0.1% sodium azide
Quality LevelMQ100
Applications
Application ReferencesSaalbach, A., et al. 2000. J. Invest. Dermatol. 115, 882. Saalbach, A., et al. 1999. Cell Tissue Res. 298, 307. Saalbach, A., et al. 1998. Arch. Dermatol. Res. 290, 360. Saalbach, A., et al. 1997. Cell Tissue Res. 290, 539. Saalbach, A., et al. 1996. J. Invest. Dermatol. 106, 1314.
Key Applications Flow Cytometry
Frozen Sections
Immunoblotting (Western Blotting)
Immunofluorescence
Immunoprecipitation
Application NotesFlow Cytometry (see comments)
Frozen Sections (10 µg/ml, acetone fixation)
Immunoblotting (10 µg/ml, non-reducing only)
Immunofluorescence (10 µg/ml)
Immunoprecipitation (2-10 µg/ml)
Application CommentsThis antibody has been used with magnetic beads to remove fibroblasts from mixed cultures. It is also suitable for flow cytometry studies (see application references). Note that in healthy tissue (skin, placenta, liver, heart), this antibody does not react with endothelial cells. However, endothelial cells activated by PMA, TNFα, or IFNγ may be detected by this antibody, suggesting activated endothelial cells may express CD90 and that CD90 may be an activation marker of human endothelial cells. Antibody should be titrated for optimal results in individual systems.
Biological Information
Immunogenhuman fibroblasts
ImmunogenHuman
CloneAS02
HostMouse
IsotypeIgG₁
Species Reactivity
  • Human
  • Monkey
Antibody TypeMonoclonal Antibody
Concentration Label Please refer to vial label for lot-specific concentration
Storage and Shipping Information
Ship Code Blue Ice Only
Toxicity Standard Handling
Storage +2°C to +8°C
Do not freeze Ok to freeze
Global Trade Item Number
Katalogové číslo GTIN
CP28 0