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MABE227 Anti-FTO Antibody, clone 5-2H10

Anti-FTO Antibody, clone 5-2H10 detects level of FTO & has been published & validated for use in Western Blotting, IHC(P).

Anti-FTO Antibody, clone 5-2H10 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Epigenetics & Nuclear Function Research Sub-Category: Cell Cycle, DNA Replication & Repair Gene Symbol: FTO UniProt Number: Q9C0B1
MABE227
100 µg  
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Tabulka spec. kláve

Species ReactivityKey ApplicationsHostFormatAntibody Type
H, RWB, IH(P)MPurifiedMonoclonal Antibody
Description
Catalogue NumberMABE227
DescriptionAnti-FTO Antibody, clone 5-2H10
Alternate Names
  • Alpha-ketoglutarate-dependent dioxygenase FTO
  • Fat mass and obesity-associated protein
Background InformationAlpha-ketoglutarate-dependent dioxygenase FTO (Fat mass and obesity-associated protein) is a ubiquitous nuclear protein which belongs to the FTO family. It is a dioxygenase enzyme that dealkylates nucleic acids by oxidative demethylation in the presence of oxygen, alpha-ketoglutarate and iron (Fe2+). FTO is highly active on single-stranded RNA molecules with 3-methyluracil residues and on single-stranded DNA containing 3-methylthymine residues, and has relatively low activity on single-stranded DNA containing either 3-methylcytosine or 3-methyladenine. There has been no observed activity on double-stranded DNA. FTO is highly abundant in the hypothalamus, pituitary, and adrenal glands. It plays an important role in energy homeostasis and the regulation of fat accumulation in the body, and may be involved in obesity. Mutated FTO proteins are linked to a syndrome characterized by retarded growth, delayed development, coarse facies and early death.
References
Product Information
FormatPurified
Control
  • Human kidney tissue lysate
PresentationPurified mouse monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationAnti-FTO Antibody, clone 5-2H10 detects level of FTO & has been published & validated for use in Western Blotting, IHC(P).
Key Applications
  • Western Blotting
  • Immunohistochemistry (Paraffin)
Application NotesImmunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected FTO in glandular cells of rat pituitary gland tissue.
Biological Information
ImmunogenRecombinant protein corresponding to human FTO.
Clone5-2H10
ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
HostMouse
IsotypeIgG2aκ
Species Reactivity
  • Human
  • Rat
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Entrez Gene SummaryThis gene is a nuclear protein of the AlkB related non-haem iron and 2-oxoglutarate-dependent oxygenase superfamily but the exact physiological function of this gene is not known. Other non-heme iron enzymes function to reverse alkylated DNA and RNA damage by oxidative demethylation. Studies in mice and humans indicate a role in nervous and cardiovascular systems and a strong association with body mass index, obesity risk, and type 2 diabetes.
Gene Symbol
  • FTO
Purification MethodProtein G Purified
UniProt Number
UniProt SummaryFUNCTION: Dioxygenase that repairs alkylated DNA and RNA by oxidative demethylation. Has highest activity towards single-stranded RNA containing 3-methyluracil, followed by single-stranded DNA containing 3-methylthymine. Has low demethylase activity towards single-stranded DNA containing 1-methyladenine or 3-methylcytosine. Has no activity towards 1-methylguanine. Has no detectable activity towards double-stranded DNA. Requires molecular oxygen, alpha-ketoglutarate and iron. Contributes to the regulation of the global metabolic rate, energy expenditure and energy homeostasis. Contributes to the regulation of body size and body fat accumulation.

COFACTOR: Binds 1 Fe2+ ion per subunit.

ENZYME REGULATION: Activated by ascorbate. Inhibited by N-oxalylglycine, fumarate and succinate.

SUBUNIT STRUCTURE: Monomer. May also exist as homodimer.

SUBCELLULAR LOCATION: Nucleus.

TISSUE SPECIFICITY: Ubiquitously expressed, with relatively high expression in adrenal glands and brain; especially in hypothalamus and pituitary.

DOMAIN: The 3D-structure of the Fe2OG dioxygenase domain is similar to that of the Fe2OG dioxygenase domain found in the bacterial DNA repair dioxygenase alkB and its mammalian orthologs, but sequence similarity is very low. As a consequence, the domain is not detected by protein signature databases.
Molecular Weight~58 kDa observed.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blot in human kidney tissue lysate.

Western Blot Analysis: 1 µg/mL of this antibody detected FTO in 10 µg of human kidney tissue lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 µg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Katalogové číslo GTIN
MABE227 04053252594915

Documentation

Anti-FTO Antibody, clone 5-2H10 MSDS

Title

Safety Data Sheet (SDS) 

Anti-FTO Antibody, clone 5-2H10 Certificates of Analysis

TitleLot Number
Anti-FTO, clone 5-2H10 - 2385976 2385976
Anti-FTO, clone 5-2H10 - 2295485 2295485
Anti-FTO, clone 5-2H10 - 3288501 3288501
Anti-FTO, clone 5-2H10 - 3384549 3384549
Anti-FTO, clone 5-2H10 - 3524847 3524847
Anti-FTO, clone 5-2H10 - 3916197 3916197
Anti-FTO, clone 5-2H10 - Q1984921 Q1984921
Anti-FTO, clone 5-2H10 -2586547 2586547
Anti-FTO, clone 5-2H10 -2801049 2801049
Anti-FTO, clone 5-2H10 -2859159 2859159
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Brochure

Title
New Products: Volume 3, 2012