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MABS460-I-25UG Anti-FGF23 Antibody, clone 278-2

Anti-FGF23, clone 278-2, Cat. No. MABS460-I, is a mouse monoclonal antibody that detects Fibroblast growth factor 23 and is tested for use in Flow Cytometry and Western Blotting.

MABS460-I-25UG
25 μg  
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Description
Catalogue NumberMABS460-I-25UG
DescriptionAnti-FGF23 Antibody, clone 278-2
Alternate Names
  • Fibroblast growth factor 23
  • FGF-23
  • Phosphatonin
  • Tumor-derived hypophosphatemia-inducing factor
Background InformationFibroblast growth factor 23 (UniProt: Q9GZV9; also known as FGF-23, Phosphatonin, Tumor-derived hypophosphatemia-inducing factor) is encoded by the FGF23 (also known as HYPF, UNQ3027/PRO9828) gene (Gene ID: 8074) in human. FGF23 is a growth factor that is produced primarily in the bone tissue and its primary hormonal function is to regulate phosphate homeostasis and vitamin D metabolism. Unlike other FGFs, it has a low affinity for heparin sulfate, which allows it to enter the circulation and act as an endocrine hormone. It inhibits renal tubular phosphate transport by reducing sodium-dependent phosphate transport protein 2A (SLC34A1) levels. FGF23 binding to FGFR requires a co‐receptor, Klotho, which acts as a scaffold for docking with FGFR1. Together with Klotho, it modulates phosphate reabsorption and both 1 ‐hydroxylation and 24‐hydroxylation in the renal proximal tubules. FGF23 is reported to inhibit the expression of 1 ‐hydroxylase while increasing the expression of 24‐hydroxylase, thus reducing the concentration of active 1,25(OH)2D. It can also act directly on the parathyroid to reduce parathyroid hormone secretion. (Ref.: Almel, EA., et al. (2019). J. Bone Min. Res (JMBR Plus). 3(8): e10190).
References
Product Information
FormatPurified
PresentationPurified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ200
Applications
ApplicationAnti-FGF23, clone 278-2, Cat. No. MABS460-I, is a mouse monoclonal antibody that detects Fibroblast growth factor 23 and is tested for use in Flow Cytometry and Western Blotting.
Key Applications
  • Western Blotting
  • Flow Cytometry
Application NotesTested Applications:\

Flow Cytometry Analysis (FC): 1 µg of this antibody detected Fibroblast growth factor 23 (FGF23) in one million Jurkat cells pretreated with Brefeldin A (1 µg/mL 4 h).
Biological Information
ImmunogenRecombinant human Fibroblast growth factor 23 (FGF23).
EpitopeUnknown
Clone278-2
Concentration1.0 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
HostMouse
SpecificityClone 278.2 is a mouse monoclonal antibody that detects human Fibroblast growth factor 23 (FGF-23).
IsotypeIgG1κ
Species Reactivity
  • Human
Species Reactivity NoteHuman.
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • FGF23
Purification MethodProtein G purified
UniProt Number
Molecular Weight~37 kDa observed; 27.95 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in Human brain tissue lysates.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected FGF23 in Human brain tissue lysates.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsRecommend storage at +2°C to +8°C. For long term storage antibodies can be kept at -20°C. Avoid repeated freeze-thaws.
Packaging Information
Material Size25 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Katalogové číslo GTIN
MABS460-I-25UG 04065266791426