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ST1579 Anti-FADS1 Mouse mAb (2D9)

Anti-FADS1, mouse monoclonal, clone 2D9, recognizes the ~50-55 kDa FADS1 n HeLa cells and human skeletal muscle tissue. It is validated for use in ELISA, WB, and IHC (paraffin sections).

Anti-FADS1 Mouse mAb (2D9) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Anti-Fatty Acid Desaturase 1

ST1579
  
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Replacement Information

Tabulka spec. kláve

Species ReactivityHostAntibody Type
HMMonoclonal Antibody
Description
Overview

This product has been discontinued.



Recognizes the ~50-55 kDa FADS1 protein in HeLa cells and human skeletal muscle tissue.
Catalogue NumberST1579
Brand Family Calbiochem®
SynonymsAnti-Fatty Acid Desaturase 1
Application Data
Detection of human FADS1 by immunoblotting. Sample: Whole cell lysates from HeLa NE cells. Primary antibody: Anti-FADS1 Mouse mAb (2D9) (Cat. No. ST1579) (1-5 µg/ml). Detection: chemiluminescence.

Detection of human FADS1 by staining paraffin sections. Sample: Formalin-fixed, paraffin-embedded human skeletal muscle. Primary antibody: Anti-FADS1 Mouse mAb (2D9) (Cat. No. ST1579) (3 µg/ml). Detection: colorimetric (DAB).
References
Product Information
FormLiquid
FormulationIn PBS, pH 7.2.
Positive controlHeLa cells, Human skeletal muscle tissue
PreservativeNone
Quality LevelMQ100
Applications
Key Applications Enzyme-Linked Immunosorbent Assay
Immunoblotting (Western Blotting)
Paraffin Sections
Application NotesELISA (see comments)
Immunoblotting (1-5 µg/ml)
Paraffin Sections (3 µg/ml)
Application CommentsFor ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
Biological Information
ImmunogenA recombinant polypeptide corresponding to amino acids 1-100 of human FADS1, expressed as a GST fusion protein
Clone2D9
HostMouse
IsotypeIgG2a
Species Reactivity
  • Human
Antibody TypeMonoclonal Antibody
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Blue Ice Only
Toxicity Regulatory Review
Storage -20°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Katalogové číslo GTIN
ST1579 0

Documentation

Anti-FADS1 Mouse mAb (2D9) MSDS

Title

Safety Data Sheet (SDS) 

Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision09-November-2010 RFH
SynonymsAnti-Fatty Acid Desaturase 1
ApplicationELISA (see comments)
Immunoblotting (1-5 µg/ml)
Paraffin Sections (3 µg/ml)
Application Data
Detection of human FADS1 by immunoblotting. Sample: Whole cell lysates from HeLa NE cells. Primary antibody: Anti-FADS1 Mouse mAb (2D9) (Cat. No. ST1579) (1-5 µg/ml). Detection: chemiluminescence.

Detection of human FADS1 by staining paraffin sections. Sample: Formalin-fixed, paraffin-embedded human skeletal muscle. Primary antibody: Anti-FADS1 Mouse mAb (2D9) (Cat. No. ST1579) (3 µg/ml). Detection: colorimetric (DAB).
DescriptionPurified mouse monoclonal antibody. Recognizes the ~50-55 kDa FADS1 protein.
BackgroundFADS1 is a member of the fatty acid desaturase (FADS) gene family. Desaturase enzymes regulate unsaturation of fatty acids through the introduction of double bonds between defined carbons of the fatty acyl chain. FADS family members are considered fusion products composed of an N-terminal cytochrome b5-like domain and a C-terminal multiple membrane-spanning desaturase portion, both of which are characterized by conserved histidine motifs.
HostMouse
ImmunogenA recombinant polypeptide corresponding to amino acids 1-100 of human FADS1, expressed as a GST fusion protein
Clone2D9
IsotypeIgG2a
Specieshuman
Positive controlHeLa cells, Human skeletal muscle tissue
FormLiquid
FormulationIn PBS, pH 7.2.
PreservativeNone
CommentsFor ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
Storage Avoid freeze/thaw
-20°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Toxicity Regulatory Review