Millipore Sigma Vibrant Logo
Attention: We have moved. Merck Millipore products are no longer available for purchase on MerckMillipore.com.Learn More

324610 Anti-Early Endosomal Antigen 1 (1391-1410) Rabbit pAb

Anti-Early Endosomal Antigen 1 (1391-1410) Rabbit pAb MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Anti-EEA1

324610
  
Purchase on Sigma-Aldrich

Přehled

Replacement Information

Tabulka spec. kláve

Host
Rb
Description
Overview

This product has been discontinued.





Recognizes the ~160 kDa EEA1 protein.
Catalogue Number324610
Brand Family Calbiochem®
SynonymsAnti-EEA1
Application Data
Detection of mouse early endosomal antigen 1 by immunoblotting. Samples: Whole tissue extract from mouse brain left untreated (lane 1) and treated with neutralizing peptide (lane 2). Primary antibody: Anti-Early Endosomal Antigen 1 (1391-1410) Rabbit pAb (Cat. No. 324610) (1 µg/ml). Detection: chemiluminescence.
References
ReferencesMills, I.G., et al. 1998. Curr. Biol. 8, 881.
Stenmark, H., et al. 1996. J. Biol. Chem. 271, 24048.
Product Information
FormLiquid
FormulationIn PBS, 1 mg/ml BSA.
Preservative≤0.1% sodium azide
Applications
Key Applications Immunoblotting (Western Blotting)
Immunofluorescence
Application NotesImmunoblotting (1 µg/ml)
Immunofluorescence (4 µg/ml, see comments)
Application CommentsImmunofluorescence localization of EEA1 in HeLa cells shows a punctate staining pattern consistent with the cytoplasmic distribution of endosomes. Variables associated with assay conditions will dictate the optimal working dilution.
Biological Information
Immunogena synthetic peptide (TPSSKKPVRVCDACFNDLQG) corresponding to amino acids 1391-1410 of human EEA1
ImmunogenHuman
HostRabbit
IsotypeIgG
Concentration Label Please refer to vial label for lot-specific concentration
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Dry Ice Only
Toxicity Standard Handling
Storage -20°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Katalogové číslo GTIN
324610 0

Documentation

Anti-Early Endosomal Antigen 1 (1391-1410) Rabbit pAb Certificates of Analysis

TitleLot Number
324610

References

Přehled odkazů
Mills, I.G., et al. 1998. Curr. Biol. 8, 881.
Stenmark, H., et al. 1996. J. Biol. Chem. 271, 24048.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision27-July-2007 RFH
SynonymsAnti-EEA1
ApplicationImmunoblotting (1 µg/ml)
Immunofluorescence (4 µg/ml, see comments)
Application Data
Detection of mouse early endosomal antigen 1 by immunoblotting. Samples: Whole tissue extract from mouse brain left untreated (lane 1) and treated with neutralizing peptide (lane 2). Primary antibody: Anti-Early Endosomal Antigen 1 (1391-1410) Rabbit pAb (Cat. No. 324610) (1 µg/ml). Detection: chemiluminescence.
DescriptionImmunoaffinity purified rabbit polyclonal antibody. Recognizes the ~160 kDa EEA1 protein.
BackgroundEEA1 is a membrane-bound protein specific to the early endosome that is essential for fusion between early endocytic vesicles. Early endosomes are cytoplasmic compartments that fuse with endocytic vesicles for redistribution of extracellular compounds to alternate destinations. Membrane association of EEA1 is dependent on PI-3 kinase activity, inhibitors of which cause EEA1 to dissociate from early endosomes. EEA1 contains C-terminal zinc-finger-like domains similar to those found in nucleic acid binding proteins, implicating its role in membrane trafficking.
HostRabbit
Immunogen speciesHuman
Immunogena synthetic peptide (TPSSKKPVRVCDACFNDLQG) corresponding to amino acids 1391-1410 of human EEA1
IsotypeIgG
Speciescanine, hamster, human, mouse, rat
FormLiquid
FormulationIn PBS, 1 mg/ml BSA.
Concentration Label Please refer to vial label for lot-specific concentration
Preservative≤0.1% sodium azide
CommentsImmunofluorescence localization of EEA1 in HeLa cells shows a punctate staining pattern consistent with the cytoplasmic distribution of endosomes. Variables associated with assay conditions will dictate the optimal working dilution.
Storage Avoid freeze/thaw
-20°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Toxicity Standard Handling
ReferencesMills, I.G., et al. 1998. Curr. Biol. 8, 881.
Stenmark, H., et al. 1996. J. Biol. Chem. 271, 24048.