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09-774 Anti-EED Antibody

09-774
100 µg  
Purchase on Sigma-Aldrich

Speciální nabídky

Přehled

Speciální nabídky

Tabulka spec. kláve

Species ReactivityKey ApplicationsHostFormatAntibody Type
H, MWBRbPurifiedPolyclonal Antibody
Description
Catalogue Number09-774
Replaces09-727
DescriptionAnti-EED Antibody
Alternate Names
  • embryonic ectoderm development
Background InformationPolycomb group proteins are important for maintaining transcriptional silencing. One conserved PcG complex, PRC2, is composed of several proteins including the histone methyltransferase EZH2, the WD-repeat protein EED (Embryonic ectoderm development), and the Zn-finger protein Suz12. Transcriptional repression mediated by EED involves histone deacetylation, while the EZH2 methylates histone H3 on lysine 27. EED protein is present in four isoforms. These EED isoforms selectively associate with distinct EZH2-containing complexes, resulting in differential targeting of their associated methyltransferase activity. These complexes play a role in Hox gene silencing, X-inactivation, germline development, stem cell pluripotency and cancer metastasis.
Product Information
FormatPurified
Control
  • Jurkat Cell Lysate
  • 3T3/A31 cell lysate
PresentationPurified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationUse Anti-EED Antibody (Rabbit Polyclonal Antibody) validated in WB to detect EED also known as embryonic ectoderm development.
Key Applications
  • Western Blotting
Biological Information
ImmunogenA synthetic peptide corresponding to a.a. 429-441 of human EED, conjugated to KLH.
Epitopea.a. 429-441
ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
HostRabbit
SpecificityThis antibody recognizes EED, Mr ~ 62-70 kDa.
Species Reactivity
  • Human
  • Mouse
Species Reactivity NoteHuman, mouse. Predicted to react with rat, oppossum, chicken, dog, and cow based on sequence homology.
Antibody TypePolyclonal Antibody
Entrez Gene Number
Entrez Gene SummaryThis gene encodes a member of the Polycomb-group (PcG) family. PcG family members form multimeric protein complexes, which are involved in maintaining the transcriptional repressive state of genes over successive cell generations. This protein interacts with enhancer of zeste 2, the cytoplasmic tail of integrin beta7, immunodeficiency virus type 1 (HIV-1) MA protein, and histone deacetylase proteins. This protein mediates repression of gene activity through histone deacetylation, and may act as a specific regulator of integrin function. Two transcript variants encoding distinct isoforms have been identified for this gene.
Gene Symbol
  • HEED
  • WAIT-1
  • WAIT1
  • hEED
Purification MethodPurified
UniProt Number
UniProt SummaryFunction: Polycomb group (PcG) protein. Component of the PRC2/EED-EZH2 complex, which methylates Lys9 and Lys27 of histone H3, leading to transcriptional repression of the affected target gene. The PRC2/EED-EZH2 complex may also serve as a recruiting platform for DNA methyltransferases, thereby linking two epigenetic repression systems. Genes repressed by the PRC2/EED-EZH2 complex include HOXC8, HOXA9, MYT1 and CDKN2A.
Subunit structure: Interacts with MLL. Component of the PRC2/EED-EZH2 complex, which includes EED, EZH2, SUZ12, RBBP4 and RBBP7 and possibly AEBP2. The minimum components required for methyltransferase activity of the PRC2/EED-EZH2 complex are EED, EZH2 and SUZ12. The PRC2 complex may also interact with DNMT1, DNMT3A, DNMT3B and PHF1 via the EZH2 subunit and with SIRT1 via the SUZ12 subunit. Interacts with HDAC, HDAC2, histone H1 and YY1. May interact with the MA protein of HIV-1.
Subcellular location: Nucleus. Note: Transiently colocalizes with XIST at inactive X chromosomes.
Tissue specificity: Expressed in brain, colon, heart, kidney, liver, lung, muscle, ovary, peripheral blood leukocytes, pancreas, placenta, prostate, spleen, small intestine, testis, thymus and uterus. Appears to be overexpressed in breast and colon cancer.
Molecular Weight~53 kDa observed. Uniprot describes three isoforms at 51 kDa (isoform 1), 53 kDa (isoform 2), and 46 kDa (isoform 3).
Product Usage Statements
Quality AssuranceWestern Blot Analysis: 1 μg/mL of this lot detected EED on 10 μg of Jurkat cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Packaging Information
Material Size100 µg
Global Trade Item Number
Katalogové číslo GTIN
09-774 04053252738449