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MABN1593 Anti-DGCR8 Antibody, clone 11C10.1

Anti-DGCR8 Antibody, clone 11C10.1 is an antibody against DGCR8 for use in Western Blotting.

Anti-DGCR8 Antibody, clone 11C10.1 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Neuroscience Research Sub-Category: RNA Metabolism & Binding Proteins Gene Symbol: DGCR8, DGCRK6, LP4941, C22orf12 UniProt Number: Q8WYQ5
MABN1593
100 μg  
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Tabulka spec. kláve

Species ReactivityKey ApplicationsHostFormatAntibody Type
HWBMPurifiedMonoclonal Antibody
Description
Catalogue NumberMABN1593
DescriptionAnti-DGCR8 Antibody, clone 11C10.1
Alternate Names
  • Microprocessor complex subunit DGCR8
  • DiGeorge syndrome critical region 8
  • Pasha
Background InformationMicroprocessor complex subunit DGCR8 (UniProt Q8WYQ5; also known as DiGeorge syndrome critical region 8, Pasha) is encoded by the DGCR8 (also known as DGCRK6, LP4941) gene (ORF C22orf12; Gene ID 54487) in human. The DGCR8 gene encodes a double-stranded RNA-binding protein that functions as a non-catalytic subunit of the microprocessor complex which mediates the biogenesis of microRNAs (miRNA) from the primary microRNA (pri-microRNA) transcript. miRNAs are initially transcribed as pri-microRNA with a local stem-loop that is cleaved by the microprocessor complex to produce the precursor microRNA (pre-miRNA). The microprocessor complex is comprised of the RNase Drosha and the RNA-binding protein DGCR8. DGCR8 is reported to be phosphorylated by Erk and Abl, an event that promotes DGCR8 stability and stimulates Drosha recruitment and subsequent substrate pri-microRNAs processing.
References
Product Information
FormatPurified
PresentationPurified mouse monoclonal IgG1κ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationAnti-DGCR8 Antibody, clone 11C10.1 is an antibody against DGCR8 for use in Western Blotting.
Key Applications
  • Western Blotting
Biological Information
ImmunogenGST-tagged recombinant fragment from the region between the WW and DRBM 1 domain of human DGCR8.
EpitopeRegion between WW and DRBM 1 domain.
Clone11C10.1
ConcentrationPlease refer to lot specific datasheet.
HostMouse
SpecificityExpected to react with spliced isoforms 1 and 3, but not isoform 2 of human DGCR8 reported by UniProt (Q8WYQ5).
IsotypeIgG1κ
Species Reactivity
  • Human
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • DGCR8
  • DGCRK6
  • LP4941
  • C22orf12
Purification MethodProtein G Purified
UniProt Number
Molecular Weight~120 kDa observed. 86.05 kDa (isoform 1) and 82.75 kDa (isoform 3). The detected band size is consistent with the reported apparent molecular weight (Ha, M., and Kim, V.N. (2014). Nat. Rev. Mol. Cell Biol. 15(8):509-524).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in HeLa cell lysate.

Western Blotting Analysis: 1.0 µg/mL of this antibody detected DGCR8 in 10 µg of HeLa cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Katalogové číslo GTIN
MABN1593 04055977331189