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MABT917 Anti-Connexin 43 Antibody, clone 2F5

This mouse monoclonal Anti-Connexin 43, clone 2F5, Cat. No. MABT917 is tested for use in Immunohistochemistry (Paraffin), Immunofluorescence, and Western Blotting for the detection of Connexin-43.

Anti-Connexin 43 Antibody, clone 2F5 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Cell Structure Gene Symbol: Gja1, Cx43 UniProt Number: P08050
MABT917
100 μg  
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Tabulka spec. kláve

Species ReactivityKey ApplicationsHostFormatAntibody Type
H, RIH(P), IF, WBMPurifiedMonoclonal Antibody
Description
Catalogue NumberMABT917
DescriptionAnti-Connexin 43 Antibody, clone 2F5
Alternate Names
  • Gap junction alpha-1 protein
  • CX43
  • Gap junction 43 kDa heart protein
Background InformationGap junction alpha-1 protein (UniProt: P08050; also known as Connexin-43, CX43, Gap junction 43 kDa heart protein) is encoded by the Gja1 (also known as Cxn-43) gene (Gene ID: 24392) in rat. The gap junction channels are dodecamers of transmembrane proteins of the connexin family. Connexins usually have a short half-life ( 1.5 - 5 h) and this short half-life is a key element of coupling regulation as it allows very dynamic and acute changes in gap junction regulation and gap junctional communication. In cardiomyocytes three types of connexins have been identified: Connexin 40, 43, and 45. Each of these connexins form channels with unique and specific electrophysiological properties. Connexin-43 is a multi-pass membrane protein and is the most ubiquitous connexin with widespread tissue expression pattern. Connexin-43 can be phosphorylated at multiple sites and phosphorylation is shown to regulate the kinetics of its trafficking, assembly, gating and turnover in a cell-cycle-stage specific manner. Phosphorylation at Ser325, Ser328 and Ser330 by casein kinase I is reported to modulate gap junction assembly while phosphorylation of Ser368 by protein kinase C gamma isoform leads to disassembly of gap junction plaques and inhibition of gap junction activity. During myocardial ischemia connexin-43 is shown to become dephosphorylated that allows gap junctions to be opened and convey the propagation of ischemia injury. In bladder smooth muscle cells, connexin-43 displays a circadian cycle with low levels during the sleep phase.
References
Product Information
FormatPurified
PresentationPurified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationThis mouse monoclonal Anti-Connexin 43, clone 2F5, Cat. No. MABT917 is tested for use in Immunohistochemistry (Paraffin), Immunofluorescence, and Western Blotting for the detection of Connexin-43.
Key Applications
  • Immunohistochemistry (Paraffin)
  • Immunofluorescence
  • Western Blotting
Application NotesImmunohistochemistry Analysis: A 1:50 dilution from a representative lot detected Connexin 43 in rat heart tissue.

Western Blotting Analysis: A representative lot detected Connexin 43 in mouse heart tissue lysates, NRK cell lysates, and MDCK cell lysates (Courtesy of Joell Solan in Paul Lampe's Lab at Fred Hutchinson Cancer Research Center, Seattle, WA).

Immunofluorescence Analysis: A representative lot detected Connexin 43 in NRK cells (Courtesy of Joell Solan in Paul Lampe's Lab at Fred Hutchinson Cancer Research Center, Seattle, WA).
Biological Information
ImmunogenA recombinant fragment corresponding to 136 amino acids from the C-terminal half of Rat connexin-43.
EpitopeC-terminus
Clone2F5
ConcentrationPlease refer to lot specific datasheet.
HostMouse
SpecificityClone 2F5 detects connexin-43 in human and rat heart. It targets an epitope within 136 amino acids from the C-terminal half.
IsotypeIgG1κ
Species Reactivity
  • Human
  • Rat
Species Reactivity NoteHuman, Rat
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • Gja1
  • Cx43
Purification MethodProtein G purified
UniProt Number
Molecular Weight43.03 kDa calculated.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Immunohistochemistry in human heart tissue.

Immunohistochemistry Analysis: A 1:50 dilution of this antibody detected Connexin 43 in human heart tissue.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Katalogové číslo GTIN
MABT917 04054839198960