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MABT366 Anti-Claudin-1/CLDN1 Antibody, clone 7A5

This Anti-Claudin-1 Antibody, clone 7A5 is validated for use in Immunocytochemistry, Western Blotting, Flow Cytometry for the detection of CLDN1.

Anti-Claudin-1/CLDN1 Antibody, clone 7A5 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Cell Structure Research Sub-Category: Infectious Diseases - Viral Gene Symbol: CLDN1, CLD1, ILVASC, NISCH, SEMP1, UNQ481/PRO944 UniProt Number: O95832
MABT366
100 μg  
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Tabulka spec. kláve

Species ReactivityKey ApplicationsHostFormatAntibody Type
HICC, WB, FCMPurifiedMonoclonal Antibody
Description
Catalogue NumberMABT366
DescriptionAnti-Claudin-1/CLDN1 Antibody, clone 7A5
Alternate Names
  • Claudin-1
  • Senescence-associated epithelial membrane protein
Background InformationClaudin-1 (UniProt O95832; also known as Senescence-associated epithelial membrane protein) is encoded by the CLDN1 (also known as CLD1, ILVASC, NISCH, SEMP1) gene (ORF UNQ481/PRO944; Gene ID 9076) in human. Tight junctions (TJs) are highly specialized membrane barriers that block the free passage of small molecules, microorganisms, and cells across the paracellular space, as well as provide a physical barrier between the apical and basolateral membrane domains of a polarized epithelial cell. Claudins are TJ proteins whose main function is to regulate the passage of ions across the paracellular space. There exist at least 27 human claudins with differential epithelial expression patterns, allowing tissue-specific paracellular transport of ions. Claudin-1 is expressed on apical and basolateral surfaces of hepatocytes in normal liver tissue. Claudin-1 is a known host factor for hepatitis C virus (HCV) entry via interaction with CD81 and cell-to-cell HCV transmission. Claudin-1 is a four-transmembrane (a.a. 8-28, 82-102, 116-136, 164-184) protein, having both its N- and C-terminal ends exposed cytoplasmically (a.a. 1-7 & 185-211).
References
Product Information
FormatPurified
PresentationPurified mouse monoclonal IgG1κ antibody in PBS without preservatives.
Quality LevelMQ100
Applications
ApplicationThis Anti-Claudin-1 Antibody, clone 7A5 is validated for use in Immunocytochemistry, Western Blotting, Flow Cytometry for the detection of CLDN1.
Key Applications
  • Immunocytochemistry
  • Western Blotting
  • Flow Cytometry
Application NotesFlow Cytometry Analysis: 5.0 µg/mL from a representative lot immunostained HT1080 cells expressing exogenously transfected human claudin-1/CLDN1 and Huh-7.5.1 human hepatoma cells, but not mock-transfected HT1080 cells or non-claudin-1-expressing S7-d6 cells (Courtesy of Professor Masuo Kondoh, PhD, Osaka University, Japan).
Western Blotting Analysis: 0.5 µg/mL from a representative lot detected exogenously expressed claudin-1 in CLDN1-transfected, but not mock-transfected, HT1080 cells (Courtesy of Professor Masuo Kondoh, PhD, Osaka University, Japan).
Immunocytochemistry Analysis: A representative lot immunostained the surface of Huh-7.5.1 human hepatoma cells, but not the non-claudin-1-/CLDN1-expressing S7-A cells (Fukasawa, M., et al. (2015). J. Virol. 89(9):4866-4879).
Flow Cytometry Analysis: A representative lot specifically immunostained HT1080 cells expressing exogenously transfected human claudin-1 (CLDN1), but not HT1080 cells expressing human claudin-2, -4, -5, -6, -7, or -9, nor L cells expressing mouse claudin-1 (Fukasawa, M., et al. (2015). J. Virol. 89(9):4866-4879).
Flow Cytometry Analysis: A representative lot immunostained HEK293T transfectants expressing FLAG-tagged human claudin-1/CLDN1 and human-mouse claudin-1 chimeras with the second human extracellular loop (EL2), but not chimeras with the mouse EL2. M152L, but not V155I, mutation in human EL2 abolished the immunoreactivity (Fukasawa, M., et al. (2015). J. Virol. 89(9):4866-4879).
Western Blotting Analysis: A representative lot detected FLAG-tagged human claudin-1/CLDN1 and human-mouse claudin-1 chimeras with the second human extracellular loop (EL2), but not chimeras with the mouse EL2. M152L, but not V155I, mutation in the second human extracellular loop abolished the immunoreactivity (Fukasawa, M., et al. (2015). J. Virol. 89(9):4866-4879).
ELISA Analysis: A representative lot detected claudin-1/CLDN1 immunoreactivity in 3.7% formaldehyde-fixed Huh-7.5.1 human hepatoma cells by "cell ELISA" (Fukasawa, M., et al. (2015). J. Virol. 89(9):4866-4879).
Neutralization Analysis: A representative lot inhibited HCV infection of cultured Huh-7.5.1 human hepatoma cells in vitro and of human liver-chimeric mice in vivo (Fukasawa, M., et al. (2015). J. Virol. 89(9):4866-4879).
Biological Information
ImmunogenRecombinant human Claudin-1/CLDN1.
EpitopeSecond extracellular loop.
Clone7A5
ConcentrationPlease refer to lot specific datasheet.
HostMouse
SpecificityClone 7A5 specifically recognized human claudin-1/CLDN1 by targeting an epitope in the second extracellular loop and prevented claudin-1-dependent hepatitis C virus (HCV) entry into host cells. Clone 7A5 does not react with human claudin-2, -4, -5, -6, -7, or -9, nor mouse claudin-1 (Fukasawa, M., et al. (2015). J. Virol. 89(9):4866-4879).
IsotypeIgG1κ
Species Reactivity
  • Human
Species Reactivity NoteHuman. Not Mouse.
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • CLDN1
  • CLD1
  • ILVASC
  • NISCH
  • SEMP1
  • UNQ481/PRO944
Purification MethodProtein G purified.
UniProt Number
Molecular Weight~20 kDa observed. 22.74 kDa calculated.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Immunocytochemistry in HepG2 cells.

Immunocytochemistry Analysis: 10 µg/mL of this antibody detected Claudin-1/CLDN1 in HepG2 cells.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Packaging Information
Material Size100 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Katalogové číslo GTIN
MABT366 04055977351576

Documentation

Anti-Claudin-1/CLDN1 Antibody, clone 7A5 MSDS

Title

Safety Data Sheet (SDS) 

Anti-Claudin-1/CLDN1 Antibody, clone 7A5 Certificates of Analysis

TitleLot Number
Anti-Claudin-1/CLDN1, clone 7A5 - 3328865 3328865
Anti-Claudin-1/CLDN1, clone 7A5 - 3784454 3784454
Anti-Claudin-1/CLDN1, clone 7A5 - 3840850 3840850
Anti-Claudin-1/CLDN1, clone 7A5 - 3886613 3886613
Anti-Claudin-1/CLDN1, clone 7A5 - 3928109 3928109
Anti-Claudin-1/CLDN1, clone 7A5 - 4023282 4023282
Anti-Claudin-1/CLDN1, clone 7A5 - 4128220 4128220
Anti-Claudin-1/CLDN1, clone 7A5 - 4206940 4206940
Anti-Claudin-1/CLDN1, clone 7A5 -Q2688172 Q2688172