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AP1151 Anti-CXCL1 Mouse mAb (7A11)

AP1151
  
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Replacement Information

Tabulka spec. kláve

Host
M
Description
Overview

This product has been discontinued.





Recognizes the CXCL1 protein.
Catalogue NumberAP1151
Brand Family Calbiochem®
SynonymsAnti-Chemokine (C-X-C) Ligand 1
Application Data
Detection of human CXCL1 by immunoblotting. Sample: Partial recombinant human protein. Primary antibody: Anti-CXCL1 Mouse mAb (7A11) (Cat. No. AP1151) (1-5 µg/ml). Detection: chemiluminescence.
References
Product Information
FormLiquid
FormulationIn PBS, pH 7.2.
PreservativeNone
Applications
Key Applications Enzyme-Linked Immunosorbent Assay
Immunoblotting (Western Blotting)
Application NotesELISA (see comments)
Immunoblotting (1-5 µg/ml)
Application CommentsFor ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
Biological Information
ImmunogenA recombinant polypeptide corresponding to amino acids 36-108 of human CXCL1, expressed as a GST fusion protein
Clone7A11
HostMouse
IsotypeIgG₁
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Blue Ice Only
Toxicity Regulatory Review
Storage -20°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Katalogové číslo GTIN
AP1151 0

Documentation

Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision19-October-2010 RFH
SynonymsAnti-Chemokine (C-X-C) Ligand 1
ApplicationELISA (see comments)
Immunoblotting (1-5 µg/ml)
Application Data
Detection of human CXCL1 by immunoblotting. Sample: Partial recombinant human protein. Primary antibody: Anti-CXCL1 Mouse mAb (7A11) (Cat. No. AP1151) (1-5 µg/ml). Detection: chemiluminescence.
DescriptionPurified mouse monoclonal antibody. Recognizes the CXCL1 protein.
BackgroundCXCL1 is secreted by human melanoma cells, has mitogenic properties and is implicated in melanoma pathogenesis. CXCL1 is expressed by macrophages, neutrophils and epithelial cells, and has neutrophil chemoattractant activity. CXCL1 plays a role in spinal cord development by inhibiting the migration of oligodendrocyte precursors and is involved in the processes of angiogenesis, inflammation, wound healing, and tumorigenesis. This chemokine elicits its effects by signaling through the chemokine receptor CXCR2. The gene for CXCL1 is located on human chromosome 4 amongst genes for other CXC chemokines. An initial study in mice showed evidence that CXCL1 decreased the severity of multiple sclerosis and may offer a neuro-protective function.
HostMouse
ImmunogenA recombinant polypeptide corresponding to amino acids 36-108 of human CXCL1, expressed as a GST fusion protein
Clone7A11
IsotypeIgG₁
Specieshuman
FormLiquid
FormulationIn PBS, pH 7.2.
PreservativeNone
CommentsFor ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
Storage Avoid freeze/thaw
-20°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Toxicity Regulatory Review