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ST1515 Anti-CAMK1 Mouse mAb (3G1)

ST1515
  
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Replacement Information

Tabulka spec. kláve

Host
M
Description
Overview

This product has been discontinued.





Recognizes the ~38 kDa CAMK1 protein in HL-60 cells.
Catalogue NumberST1515
Brand Family Calbiochem®
SynonymsAnti-Calcium/Calmodulin Dependent Protein Kinase 1
Application Data
Detection of human CAMK1 by immunoblotting. Sample: Whole cell lysate from HL-60 cells. Primary antibody: Anti-CAMK1 Mouse mAb (3G1) (Cat. No. ST1515) (1-5 µg/ml). Detection: chemiluminescence.
References
Product Information
Formliquid
FormulationIn PBS, pH 7.2.
Positive controlHL-60 cells
PreservativeNone
Applications
Key Applications Enzyme-Linked Immunosorbent Assay
Immunoblotting (Western Blotting)
Application NotesELISA (see comments)
Immunoblotting (1-5 µg/ml)
Application CommentsFor ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
Biological Information
ImmunogenA recombinant polypeptide corresponding to amino acids partial 271-371 of human CAMK1, expressed as a GST fusion protein
ImmunogenNA
Clone3G1
HostMouse
IsotypeIgG₁
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Blue Ice Only
Toxicity Regulatory Review
Storage -20°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Katalogové číslo GTIN
ST1515 0

Documentation

Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision05-November-2010 RFH
SynonymsAnti-Calcium/Calmodulin Dependent Protein Kinase 1
ApplicationELISA (see comments)
Immunoblotting (1-5 µg/ml)
Application Data
Detection of human CAMK1 by immunoblotting. Sample: Whole cell lysate from HL-60 cells. Primary antibody: Anti-CAMK1 Mouse mAb (3G1) (Cat. No. ST1515) (1-5 µg/ml). Detection: chemiluminescence.
DescriptionPurified mouse monoclonal antibody. Recognizes the ~38 kDa CAMK1 protein.
BackgroundCalcium/calmodulin-dependent protein kinase I is expressed in many tissues and is a component of a calmodulin-dependent protein kinase cascade. Calcium/calmodulin directly activates calcium/calmodulin-dependent protein kinase I by binding to the enzyme and indirectly promotes the phosphorylation and synergistic activation of the enzyme by calcium/calmodulin-dependent protein kinase I kinase.
HostMouse
Immunogen speciesNA
ImmunogenA recombinant polypeptide corresponding to amino acids partial 271-371 of human CAMK1, expressed as a GST fusion protein
Clone3G1
IsotypeIgG₁
Specieshuman
Positive controlHL-60 cells
Formliquid
FormulationIn PBS, pH 7.2.
PreservativeNone
CommentsFor ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
Storage Avoid freeze/thaw
-20°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Toxicity Regulatory Review