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PC529 Anti-BACE (Ab-2) (485-501) Rabbit pAb

Anti-BACE (Ab-2) (485-501) Rabbit pAb MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Anti-β-Amyloid Precursor Protein Cleaving Enzyme, Anti-ASP2, Anti-β-Secretase

PC529
  
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Přehled

Replacement Information

Tabulka spec. kláve

Host
Rb
Description
Overview

This product has been discontinued.





Recognizes the ~70-75 kDa BACE protein in human brain tissue extracts and mouse NIH3T3 cell lysates.
Catalogue NumberPC529
Brand Family Calbiochem®
SynonymsAnti-β-Amyloid Precursor Protein Cleaving Enzyme, Anti-ASP2, Anti-β-Secretase
Application Data
Detection of human BACE by immunoblotting. Sample: Whole tissue extract from human brain (lanes A,B) and whole cell lysate from NIH3T3 cells (lane C). Primary antibody: Anti-BACE (Ab-2) (485-501) Rabbit pAb (Cat. No. PC529) (1 µg/ml) pre-incubated without blocking peptide (lanes A,C) or with blocking peptide (lane B). Detection: chemiluminescence.
References
ReferencesHussain, I., et al. 1999. Mol. Cell. Neurosci. 14, 419.
Sinha, S., et al. 1999. Nature. 502, 537.
Vassar, R., et al. 1999. Science. 266, 735.
Yan, R., et al. 1999. Nature 402, 583.
Product Information
FormLiquid
FormulationIn PBS.
Positive controlHuman brain tissue lysate
Preservative≤0.1% sodium azide
Applications
Key Applications Immunoblotting (Western Blotting)
Application NotesImmunoblotting (1:500-1:1000)
Application CommentsAntibody should be titrated for optimal results in individual systems.
Biological Information
Immunogena synthetic peptide (CLRQQHDDFADDISLLK) corresponding to amino acids 485-501 of human BACE
ImmunogenHuman
HostRabbit
IsotypeIgG
Concentration Label Please refer to vial label for lot-specific concentration
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Dry Ice Only
Toxicity Standard Handling
Storage -20°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Katalogové číslo GTIN
PC529 0

Documentation

Anti-BACE (Ab-2) (485-501) Rabbit pAb MSDS

Title

Safety Data Sheet (SDS) 

Anti-BACE (Ab-2) (485-501) Rabbit pAb Certificates of Analysis

TitleLot Number
PC529

References

Přehled odkazů
Hussain, I., et al. 1999. Mol. Cell. Neurosci. 14, 419.
Sinha, S., et al. 1999. Nature. 502, 537.
Vassar, R., et al. 1999. Science. 266, 735.
Yan, R., et al. 1999. Nature 402, 583.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision11-October-2007 RFH
SynonymsAnti-β-Amyloid Precursor Protein Cleaving Enzyme, Anti-ASP2, Anti-β-Secretase
ApplicationImmunoblotting (1:500-1:1000)
Application Data
Detection of human BACE by immunoblotting. Sample: Whole tissue extract from human brain (lanes A,B) and whole cell lysate from NIH3T3 cells (lane C). Primary antibody: Anti-BACE (Ab-2) (485-501) Rabbit pAb (Cat. No. PC529) (1 µg/ml) pre-incubated without blocking peptide (lanes A,C) or with blocking peptide (lane B). Detection: chemiluminescence.
DescriptionRabbit polyclonal antibody purified by ion exchange chromotography. Recognizes the ~70-75 kDa BACE protein.
BackgroundAccumulation of the amyloid-β(Aβ) plaque in the cerebral cortex is a critical event in the pathogenesis of Alzheimer's disease. Aβ peptide is generated by proteolytic cleavage of the β-amyloid protein precursor (APP) at β- and γ-sites by two proteases. APP is first cleaved by β-secretase, producing a soluble derivative of the protein and a membrane anchored 99-amino acid carboxy-terminal fragment (C99). The C99 fragment serves as a substrate for γ-secretase to generate the ~4 kDa amyloid-βpeptide, which is deposited in the brains of all suffers of Alzheimers disease. The long-sought β-secretase was recently identified by several groups independently and designated beta-site APP cleaving enzyme (BACE) and aspartyl protease 2 (Asp2). BACE, a ~70 kDa protein, is a novel transmembrane aspartic protease that colocalizes with APP.
HostRabbit
Immunogen speciesHuman
Immunogena synthetic peptide (CLRQQHDDFADDISLLK) corresponding to amino acids 485-501 of human BACE
IsotypeIgG
Specieshuman, mouse
Positive controlHuman brain tissue lysate
FormLiquid
FormulationIn PBS.
Concentration Label Please refer to vial label for lot-specific concentration
Preservative≤0.1% sodium azide
CommentsAntibody should be titrated for optimal results in individual systems.
Storage Avoid freeze/thaw
-20°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Toxicity Standard Handling
ReferencesHussain, I., et al. 1999. Mol. Cell. Neurosci. 14, 419.
Sinha, S., et al. 1999. Nature. 502, 537.
Vassar, R., et al. 1999. Science. 266, 735.
Yan, R., et al. 1999. Nature 402, 583.