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MABE143 Anti-Ago1 Antibody, clone 4G7-E12

Anti-Ago1 Antibody, clone 4G7-E12 is a Mouse Monoclonal Antibody for detection of Ago1 also known as hAgo1, Argonaute1, eIF-2C 1 & has been validated in WB.

Anti-Ago1 Antibody, clone 4G7-E12 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Epigenetics & Nuclear Function Research Sub-Category: Cell Cycle, DNA Replication & Repair Gene Symbol: EIF2C1, AGO1 UniProt Number: Q9UL18
MABE143
100 µg  
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Tabulka spec. kláve

Species ReactivityKey ApplicationsHostFormatAntibody Type
HWBMPurifiedMonoclonal Antibody
Description
Catalogue NumberMABE143
DescriptionAnti-Ago1 Antibody, clone 4G7-E12
Alternate Names
  • hAgo1
  • Argonaute1
  • eIF-2C 1
  • eIF2C 1
  • Q99
  • GERP95
  • DKFZp686M13167
Background InformationAgo1 (Eukaryotic translation initiation factor 2C 1) belongs to the argonaute family and contains 1 PAZ and 1 Piwi domain. It is required for G1 arrest and mating in response to nitrogen starvation. It is also known as a cell cycle control protein through its regulation of cytokinesis and its related cell cycle checkpoints, which occur downstream of dcr1. It is required, indirectly, for regulated hyperphosphorylation of cdc2. Ago 1 also has a role in the RNA interference (RNAi) pathway, which is important for heterochromatin formation and accurate chromosome segregation. It has been shown to be non-essential for siRNA-directed target RNA cleavage, but is needed for the production of mature miRNA that affects miRNA-directed RNA cleavage.
References
Product Information
FormatPurified
Control
  • HeLa cell lysate
PresentationPurified mouse monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationAnti-Ago1 Antibody, clone 4G7-E12 is a Mouse Monoclonal Antibody for detection of Ago1 also known as hAgo1, Argonaute1, eIF-2C 1 & has been validated in WB.
Key Applications
  • Western Blotting
Application Notes Immunoprecipitation: A representative lot of this antibody was used for immunoprecipitation by an independent laboratory (see Azuma-Mukai A. et al. (2008) Proc. Natl. Acad. Sci. USA 105: 7964-7969).
Biological Information
ImmunogenGST-tagged recombinant protein corresponding to human Ago1.
Clone4G7-E12
ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
HostMouse
SpecificityThis antibody has been shown by an independent laboratory to not cross react with Ago2, Ago3, or Ago4 (Azuma-Mukai A et al. (2008) Proc. Natl. Acad. Sci. USA 105: 7964-7969).
IsotypeIgG2aκ
Species Reactivity
  • Human
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Entrez Gene SummaryThis gene encodes a member of the Argonaute family of proteins which play a role in RNA interference. The encoded protein is highly basic, and contains a PAZ domain and a PIWI domain. It may interact with dicer1 and play a role in short-interfering-RNA-mediated gene silencing. This gene is located on chromosome 1 in a cluster of closely related family members including argonaute 3, and argonaute 4.
Gene Symbol
  • EIF2C1
  • AGO1
Purification MethodProtein G Purified
UniProt Number
UniProt SummaryFUNCTION: Required for RNA-mediated gene silencing (RNAi). Binds to short RNAs such as microRNAs (miRNAs) or short interfering RNAs (siRNAs), and represses the translation of mRNAs which are complementary to them. Lacks endonuclease activity and does not appear to cleave target mRNAs. Also required for transcriptional gene silencing (TGS) of promoter regions which are complementary to bound short antigene RNAs (agRNAs).

SUBUNIT STRUCTURE: Interacts with DDB1, DDX6, DDX20, DDX47, DHX9, DICER1, EIF2C2/AGO2, GEMIN4, ILF3, IMP8, MOV10, PRMT5, SART3, TARBP2, TNRC6B and UPF1.

SUBCELLULAR LOCATION: Cytoplasm > P-body.
Molecular Weight~97 kDa observed.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blot in HeLa cell lysate.

Western Blot Analysis: 0.1 µg/mL of this antibody detected Ago1 in HeLa cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 µg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Katalogové číslo GTIN
MABE143 04053252632853

Documentation

Anti-Ago1 Antibody, clone 4G7-E12 MSDS

Title

Safety Data Sheet (SDS) 

Anti-Ago1 Antibody, clone 4G7-E12 Certificates of Analysis

TitleLot Number
Anti-Ago1, clone 4G7-E12 3073362
Anti-Ago1, clone 4G7-E12 - 2366607 2366607
Anti-Ago1, clone 4G7-E12 - 2205032 2205032
Anti-Ago1, clone 4G7-E12 - 2287218 2287218
Anti-Ago1, clone 4G7-E12 - 3744823 3744823
Anti-Ago1, clone 4G7-E12 - 4123908 4123908
Anti-Ago1, clone 4G7-E12 - 4153073 4153073
Anti-Ago1, clone 4G7-E12 - NRG1911091 NRG1911091
Anti-Ago1, clone 4G7-E12 -2620668 2620668
Anti-Ago1, clone 4G7-E12 -2788123 2788123

Brochure

Title
New Products: Volume 3, 2012