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MABS1166 Anti-AdipoR2 Antibody, clone 4D3.2

This Anti-AdipoR2 Antibody, clone 4D3.2 is validated for use in Western Blotting, Immunohistochemistry (Paraffin) for the detection of AdipoR2.

Anti-AdipoR2 Antibody, clone 4D3.2 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Signaling Research Sub-Category: Lipid Metabolism & Weight Regulation Gene Symbol: ADIPOR2, PAQR2 UniProt Number: Q86V24
MABS1166
100 µL  
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Tabulka spec. kláve

Species ReactivityKey ApplicationsHostFormatAntibody Type
H, RWB, IH(P)MPurifiedMonoclonal Antibody
Description
Catalogue NumberMABS1166
DescriptionAnti-AdipoR2 Antibody, clone 4D3.2
Alternate Names
  • Adiponectin receptor protein 2
  • AdipoR2
  • Progestin and adipoQ receptor family member II
Background InformationAdiponectin receptor protein 2 (UniProt Q86V24; also known as Progestin and adipoQ receptor family member II) is encoded by the ADIPOR2 (also known as PAQR2) gene (Gene ID 79602) in human. Adiponectin receptor protein 1 & 2 (AdipoR1 & AdipoR2) are receptors for adiponectin, a well knonwn anti-inflammatory adipokine highly abundant in serum. Both AdipoR1 and AdipoR2 are reported to be ubiquitously expressed with the highest expression in skeletal muscle and liver, respectively. Circulating adiponectin levels are reduced in obesity and type II diabetes, whereas weight loss elevates serum adiponectin. Adiponectin deficiency in mice is reported to exacerbate metabolic dysfunction by diet-induced obesity in some studies, while adiponectin overexpression alleviates metabolic dysfunction independent of weight loss in both genetic and diet-induced animal models of obesity. Adipor1-knockout (KO) results in increased adiposity and decreased glucose clearance in mice, whereas Adipor2-KO mice are lean and displayed improved glucose clearance. In addition to its metabolic role, adiponectin deficiency is associated with various clinical cardiovascular disorders including myocardial infarction, peripheral artery disease, and endothelial dysfunction. In a study of murine hind limb ischemia model, blood flow recovery after chronic hind limb ischemic surgery is similar among wild-type and AdipoR1-KO mice, while revascularization in AdipoR2-KO mice is severely attenuated. Human AdipoR2 is a 7-transmembrane (a.a. 148-168, 182-202, 214-234, 246-266, 274-294, 310-330, 349-369) protein with the opposite topology of G protein-coupled receptors (GPCRs), having its N-terminal end at the cytoplasmic side (a.a. 1-147) and C-terminal end at the extracellular side (a.a. 370-386). Unlike GPCRs also, adipoR1 & AdipoR2 transmembrane helices are not kinked or tilted relative to the plane of the membrane.
References
Product Information
FormatPurified
PresentationPurified mouse monoclonal IgG1κ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationThis Anti-AdipoR2 Antibody, clone 4D3.2 is validated for use in Western Blotting, Immunohistochemistry (Paraffin) for the detection of AdipoR2.
Key Applications
  • Western Blotting
  • Immunohistochemistry (Paraffin)
Application NotesImmunohistochemistry Analysis: A 1:250 dilution from a representative lot detected AdipoR2 in human prostate cancer, as well as normal rat heart and human cerebral cortex tissue sections.
Biological Information
ImmunogenGST-tagged recombinant human AdipoR2 N-terminal fragment.
EpitopeN-terminal cytoplasmic domain.
Clone4D3.2
ConcentrationPlease refer to lot specific datasheet.
HostMouse
SpecificityClone 4D3.2 targets an epitope within the N-terminal cytoplasmic domain.
IsotypeIgG1κ
Species Reactivity
  • Human
  • Rat
Species Reactivity NoteHuman, Rat.
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • ADIPOR2
  • PAQR2
Purification MethodProtein G purified.
UniProt Number
Molecular Weight43.88 kDa calculated.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting of GST-tagged human AdipoR2 N-terminal fragment.

Western Blotting Analysis: A 1:1000 dilution of this antibody detected 50 ng of GST-tagged recombinant human AdipoR2 N-terminal fragment.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Katalogové číslo GTIN
MABS1166 04055977353570