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DP08 Anti-Adenovirus 5 E1B 55 kDa Rat mAb (9C10)

DP08
  
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Tabulka spec. kláve

Species ReactivityHostAntibody Type
Adenovirus Infected CellsRMonoclonal Antibody
Description
OverviewRecognizes the ~19 kDa 2 E1B and the ~55 kDa 5 E1B adenovirus proteins in HEK293 cells. Will also immunoprecipitate associated proteins such as p53 and cyclins.
Catalogue NumberDP08
Brand Family Calbiochem®
Application Data
Detection of adenovirus 5 E1B 55 kDa protein by immunocytochemistry. Sample: HEK293 cells fixed with methanol. Primary antibody: Anti-Adenovirus 5 E1B 55 kDa Rat mAb (9C10) (Cat. No. DP08) (4 µg/ml). Detection: fluorescence (FITC).
References
ReferencesWhyte, P., et al. 1988. Nature 334, 124.
Harlow, E., et al. 1986. J. Virol. 39, 861.
Ziff, E. and Velcich, A. 1985. Cell 40, 705.
Houweling, A., et al. 1980. Virology 105, 537.
Berk, A.J., et al. 1979. Cell 17, 935.
Jones, N. and Shenk, T. 1979. Proc. Natl. Acad. Sci. USA 76, 3665.
Shiroki, K., et al. 1979. Virology 95, 127.
Gallimore, P.H., et al. 1974. J. Mol. Biol. 89, 49.
Graham, F.L., et al. 1974. Biol. 39, 637.
Product Information
FormLiquid
FormulationIn 50 mM sodium phosphate buffer, 0.2% gelatin.
Negative controlHS27 cells
Positive control293 cells
Preservative≤0.1% sodium azide
Applications
Application ReferencesImmunocytochemistry and Immunofluoresecence Zantema, A., et al. 1985. Virology 142, 44.
Key Applications Immunofluorescence
Immunoprecipitation
Application NotesImmunocytochemistry (4 µg/ml, see application references)
Immunofluorescence (4 µg/ml, see application references)
Immunoprecipitation (1-2 µg/sample)
Application CommentsThis antibody will immunoprecipitate adenovirus 2 and 5 E18 proteins and associated proteins (such as Rb and cyclins) as well as stain infected cells by immunofluorescence and immunoperoxidase. Antibody should be titrated for optimal results in individual systems.
Biological Information
Immunogenadenovirus 5 E1B protein
Clone9C10
HostRat
IsotypeIgG2b
Species Reactivity
  • Adenovirus Infected Cells
Antibody TypeMonoclonal Antibody
Concentration Label Please refer to vial label for lot-specific concentration
Storage and Shipping Information
Ship Code Blue Ice Only
Toxicity Standard Handling
Storage +2°C to +8°C
Do not freeze Yes
Global Trade Item Number
Katalogové číslo GTIN
DP08 0