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ST1507 Anti-AKR1B10 Mouse mAb (1A6)

Anti-AKR1B10. Mpuse monoclonal, clone 1A6 recognizes the ~37 kDa AKR1B10 protein in HepG2 and HeLa cells and human colon tissue. It is validated for ELISA, WB, ICC, and paraffin sections.

Anti-AKR1B10 Mouse mAb (1A6) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Anti-Aldo-Keto Reductase 1B10

ST1507
Purchase on Sigma-Aldrich

Přehled

Replacement Information

Tabulka spec. kláve

Species ReactivityHostAntibody Type
HMMonoclonal Antibody

Products

Katalogové čísloBalení ks/bal.
ST1507-100UG 100 μg
Description
OverviewRecognizes the ~37 kDa AKR1B10 protein in HepG2 and HeLa cells and human colon tissue.
Catalogue NumberST1507
Brand Family Calbiochem®
SynonymsAnti-Aldo-Keto Reductase 1B10
Application Data
Detection of human AKR1B10 by immunoblotting. Sample: Whole cell lysate from HepG2 cells. Primary antibody: Anti-AKR1B10 Mouse mAb (1A6) (Cat. No. ST1507) (1-5 µg/ml). Detection: chemiluminescence.

Detection of human AKR1B10 by staining paraffin sections. Sample: Formalin-fixed, paraffin-embedded human colon tissue. Primary antibody: Anti-AKR1B10 Mouse mAb (1A6) (Cat. No. ST1507) (3 µg/ml). Detection: colorimetric (DAB).

Detection of human AKR1B10 by immunocytochemistry. Sample: HeLa cellsfixed in Paraformaldehyde. Primary antibody: Anti-AKR1B10 Mouse mAb (1A6) (Cat. No. ST1507) (10 µg/ml). Detection: fluorescence.
References
ReferencesAhn K.H., et al. 2010 JOURNAL OF HEALTH SCIENCE 56, 287.
Satow, R., et al. 2010. Clin. Cancer Res. 16, 2518.
Product Information
Formliquid
FormulationIn PBS, pH 7.2.
Positive controlHepG2 cells, Human Colon tissue, HeLa cells
PreservativeNone
Quality LevelMQ100
Applications
Key Applications Enzyme-Linked Immunosorbent Assay
Immunoblotting (Western Blotting)
Immunocytochemistry
Paraffin Sections
Application NotesELISA (see comments)
Immunoblotting (1-5 µg/ml)
Immunocytochemistry (10 µg/ml)
Paraffin Sections (3 µg/ml)
Application CommentsFor ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
Biological Information
ImmunogenA recombinant polypeptide corresponding to amino acids 76-144 of human AKR1B10, expressed as a GST fusion protein
ImmunogenNA
Clone1A6
HostMouse
IsotypeIgG2a
Species Reactivity
  • Human
Antibody TypeMonoclonal Antibody
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Blue Ice Only
Toxicity Regulatory Review
Storage -20°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Katalogové číslo GTIN
ST1507-100UG 04055977224153

Documentation

Anti-AKR1B10 Mouse mAb (1A6) MSDS

Title

Safety Data Sheet (SDS) 

References

Přehled odkazů
Ahn K.H., et al. 2010 JOURNAL OF HEALTH SCIENCE 56, 287.
Satow, R., et al. 2010. Clin. Cancer Res. 16, 2518.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision05-November-2010 RFH
SynonymsAnti-Aldo-Keto Reductase 1B10
ApplicationELISA (see comments)
Immunoblotting (1-5 µg/ml)
Immunocytochemistry (10 µg/ml)
Paraffin Sections (3 µg/ml)
Application Data
Detection of human AKR1B10 by immunoblotting. Sample: Whole cell lysate from HepG2 cells. Primary antibody: Anti-AKR1B10 Mouse mAb (1A6) (Cat. No. ST1507) (1-5 µg/ml). Detection: chemiluminescence.

Detection of human AKR1B10 by staining paraffin sections. Sample: Formalin-fixed, paraffin-embedded human colon tissue. Primary antibody: Anti-AKR1B10 Mouse mAb (1A6) (Cat. No. ST1507) (3 µg/ml). Detection: colorimetric (DAB).

Detection of human AKR1B10 by immunocytochemistry. Sample: HeLa cellsfixed in Paraformaldehyde. Primary antibody: Anti-AKR1B10 Mouse mAb (1A6) (Cat. No. ST1507) (10 µg/ml). Detection: fluorescence.
DescriptionPurified mouse monoclonal antibody. Recognizes the ~37 kDa AKR1B10 protein.
BackgroundThis gene product encodes a member of the aldo/keto reductase superfamily, which consists of more than 40 known enzymes and proteins. This member can efficiently reduce aliphatic and aromatic aldehydes, and it is less active on hexoses. It is highly expressed in adrenal gland, small intestine, and colon, and may play an important role in liver carcinogenesis.
HostMouse
Immunogen speciesNA
ImmunogenA recombinant polypeptide corresponding to amino acids 76-144 of human AKR1B10, expressed as a GST fusion protein
Clone1A6
IsotypeIgG2a
Specieshuman
Positive controlHepG2 cells, Human Colon tissue, HeLa cells
Formliquid
FormulationIn PBS, pH 7.2.
PreservativeNone
CommentsFor ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
Storage Avoid freeze/thaw
-20°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Toxicity Regulatory Review
ReferencesAhn K.H., et al. 2010 JOURNAL OF HEALTH SCIENCE 56, 287.
Satow, R., et al. 2010. Clin. Cancer Res. 16, 2518.