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AP1062 PhosphoDetect™ Anti-PDH-E1α (pSer²⁹³) Rabbit pAb

This PhosphoDetect™ Anti-PDH-E1α (pSer²⁹³) Rabbit pAb is validated for use in Immunoblotting, IP, ICC for the detection of PDH-E1α (pSer²⁹³).

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Anti-Pyruvate Dehydogenase pSer²⁹³ Rabbit pAb

AP1062
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Overview

Replacement Information

Key Spec Table

Species ReactivityHostAntibody Type
H, M, Pm, RRbPolyclonal Antibody

Pricing & Availability

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AP1062-50ULCN
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      Description
      OverviewRecognizes the ~44 kDa PDH-E1α protein phosphorylated at Ser293 in HEK293 cells.
      Catalogue NumberAP1062
      Brand Family Calbiochem®
      SynonymsAnti-Pyruvate Dehydogenase pSer²⁹³ Rabbit pAb
      Application Data
      Detection of human PDH-E3α, phosphorylated at Ser293, by immunocytochemistry. Samples: HEK293 cells treated with DCA (bottom panels) or left untreated (top panels) and subsequently fixed with 4% paraformaldehyde. Primary antibody: PhosphoDetect™ Anti-PDH-E1α (pSer293) Rabbit pAb (Cat. No. AP1062) (1:500). Detection: fluorescence (red) using DAPI to counterstain the nuclei (blue) and AlexaFluor®-Phalloidin to counterstain actin filaments (green).

      Detection of human PDH-E1α, phosphorylated at Ser293, by immunoprecipitation, followed by immunoblotting. Samples: Whole cell lysate (375 µg) from HEK293 cells. Antibody for IP: PhosphoDetect™ Anti PDH-E1α (pSer293) Rabbit pAb (Cat. No. AP1062) (1:50). . Antibodyfor IB: Anti-PDH-E1α (pSer293 Rabbit pAb (Cat. No. AP1062) (1:1000) Detection: chemiluminescence.

      Detection of human PDH-E1α, phosphorylated at Ser293, by immunoblotting. Samples: Whole cell lysate from HEK293 cells left untreated (lane 1) and HEK293 cells treated with dichloroacetate. Primary antibody: PhosphoDetect™ Anti PDH-E1α (pSer293) Rabbit pAb (Cat. No. AP1062) (1:10,000). Detection: chemiluminescence.
      References
      ReferencesSeifert, F., et al. 2007. Biochemistry 21, 6277.
      Lee, J., et al. 2007. Mol. Cell Prot. 4, 669.
      Korotchkina, L.G., et al. 2001. J. Biol. Chem. 40, 37223.
      Product Information
      FormLiquid
      FormulationIn 150 mM NaCl, 100 mM Tris-Glycine, pH 7.4.
      Positive controlHEK293 cells
      Preservative≤0.1% sodium azide
      Quality LevelMQ100
      Applications
      Key Applications Immunoblotting (Western Blotting)
      Immunocytochemistry
      Immunoprecipitation
      Application NotesImmunoblotting (1:10,000)
      Immunoprecipitation (1:50)
      Immunocytochemistry (1:500)
      Application CommentsAntibody should be titrated for optimal results in individual systems.
      Biological Information
      Immunogena synthetic phosphopeptide corresponding to amino acids surrounding the Ser 293 phosphorylation site of human PDH-E1α
      ImmunogenHuman
      HostRabbit
      IsotypeIgG
      Species Reactivity
      • Human
      • Mouse
      • Primate
      • Rat
      Antibody TypePolyclonal Antibody
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Standard Handling
      Storage -20°C
      Do not freeze Ok to freeze
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      AP1062-50ULCN 04055977227765

      Documentation

      PhosphoDetect™ Anti-PDH-E1α (pSer²⁹³) Rabbit pAb SDS

      Title

      Safety Data Sheet (SDS) 

      PhosphoDetect™ Anti-PDH-E1α (pSer²⁹³) Rabbit pAb Certificates of Analysis

      TitleLot Number
      AP1062

      References

      Reference overview
      Seifert, F., et al. 2007. Biochemistry 21, 6277.
      Lee, J., et al. 2007. Mol. Cell Prot. 4, 669.
      Korotchkina, L.G., et al. 2001. J. Biol. Chem. 40, 37223.

      Citations

      Title
      Madhok, B.M., et al. 2010. British Journal of Cancer 102, 1746.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision30-August-2011 JSW
      SynonymsAnti-Pyruvate Dehydogenase pSer²⁹³ Rabbit pAb
      ApplicationImmunoblotting (1:10,000)
      Immunoprecipitation (1:50)
      Immunocytochemistry (1:500)
      Application Data
      Detection of human PDH-E3α, phosphorylated at Ser293, by immunocytochemistry. Samples: HEK293 cells treated with DCA (bottom panels) or left untreated (top panels) and subsequently fixed with 4% paraformaldehyde. Primary antibody: PhosphoDetect™ Anti-PDH-E1α (pSer293) Rabbit pAb (Cat. No. AP1062) (1:500). Detection: fluorescence (red) using DAPI to counterstain the nuclei (blue) and AlexaFluor®-Phalloidin to counterstain actin filaments (green).

      Detection of human PDH-E1α, phosphorylated at Ser293, by immunoprecipitation, followed by immunoblotting. Samples: Whole cell lysate (375 µg) from HEK293 cells. Antibody for IP: PhosphoDetect™ Anti PDH-E1α (pSer293) Rabbit pAb (Cat. No. AP1062) (1:50). . Antibodyfor IB: Anti-PDH-E1α (pSer293 Rabbit pAb (Cat. No. AP1062) (1:1000) Detection: chemiluminescence.

      Detection of human PDH-E1α, phosphorylated at Ser293, by immunoblotting. Samples: Whole cell lysate from HEK293 cells left untreated (lane 1) and HEK293 cells treated with dichloroacetate. Primary antibody: PhosphoDetect™ Anti PDH-E1α (pSer293) Rabbit pAb (Cat. No. AP1062) (1:10,000). Detection: chemiluminescence.
      DescriptionImmunoaffinity purified rabbit polyclonal antibody. Recognizes the ~44 kDa PDH-E1α protein phosphorylated at Ser293.
      BackgroundPyruvate dehydogenase (PDH) complex (PDC) catalyzes the irreversible conversion of pyruvate to acetyl CoA, providing the link between glycolyis and the TCA cycle. Dysregulation of the PDC in skeletal muscle has been implicated in type 2 diabetes and mutations in the PDC result in various mitochondrial diseases. The PDC is a multienzyme complex composed of several copies of three catalytic proteins (E1, E2, and E3): one structural (E3-binding protein) and two regulatory proteins (PDH kinase [PDK] and PDH phosphatase [PDP]). The activity of the PDC complex is mainly regulated by phosphorylation-dehosphorylation of three specific serine residues (232, 293, 300) on the E1 subunit. Phosphorylation of PDH determines the activity of the complex. Phosphorylation by one of four PDK isoforms inactivates the complex, while removal of the phosphate by one of two PDP isoforms activates the complex. The activity of the PDP1 isoform is sensitive to Ca2+, while the PDK isoforms are regulated by the reduction and acetylation status of the E2 subunit, pyruvate, and the small molecule the anti cancer dichloroacetate. (DCA).
      HostRabbit
      Immunogen speciesHuman
      Immunogena synthetic phosphopeptide corresponding to amino acids surrounding the Ser 293 phosphorylation site of human PDH-E1α
      IsotypeIgG
      Specieshuman, mouse, primate, rat
      Positive controlHEK293 cells
      FormLiquid
      FormulationIn 150 mM NaCl, 100 mM Tris-Glycine, pH 7.4.
      Preservative≤0.1% sodium azide
      CommentsAntibody should be titrated for optimal results in individual systems.
      Storage -20°C
      Do Not Freeze Ok to freeze
      Toxicity Standard Handling
      ReferencesSeifert, F., et al. 2007. Biochemistry 21, 6277.
      Lee, J., et al. 2007. Mol. Cell Prot. 4, 669.
      Korotchkina, L.G., et al. 2001. J. Biol. Chem. 40, 37223.
      CitationMadhok, B.M., et al. 2010. British Journal of Cancer 102, 1746.