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ST1013 PhosphoDetect™ Anti-GSK-3α/β (pTyr279/216) Rabbit pAb

This PhosphoDetect™ Anti-GSK-3α/β Rabbit pAb is validated for use in Dot Blot, ELISA, WB, ICC for the detection of GSK-3α/β.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Anti-Glycogen Synthase Kinase-3α/β, Phospho-Specific (Tyr279/216)

ST1013
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Overview

Replacement Information

Key Spec Table

Species ReactivityHostAntibody Type
H, M, RRbPolyclonal Antibody

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      Description
      OverviewRecognizes the ~51 kDa GSK-3α protein phosphorylated at Tyr279 and the ~47 kDa protein GSK-3β phosphorylated at Tyr216.
      Note: 1T = 1 test. Sufficient for 10 Western miniblots.
      Catalogue NumberST1013
      Brand Family Calbiochem®
      SynonymsAnti-Glycogen Synthase Kinase-3α/β, Phospho-Specific (Tyr279/216)
      Application Data
      Detection of human GSK-3β, phosphorylated at Tyr216 by immunoblotting. Samples: Whole cell lysate from control cells (lane 1) or control lysate spiked with human recombinant GSK-3β (lanes 2-5). Primary antibody: PhosphoDetect™ Anti-Glycogen Synthase Kinase-3α/β (pTyr279/216) Rabbit pAb (Cat. No. ST1013) (1:1000) pre-incubated without peptide (lanes 1, 5), or with phosphopeptide immunogen (lane 2), a generic phosphoserine-containing peptide (lane 3), or non-phosphopeptide corresponding to the immunogen (lane 4). Detection: chemiluminescence.
      References
      ReferencesPapasozomenos, S.C., et al. 2002. PNAS 99, 1140.
      Kirschenbaum, F., et al. 2001. J. Biol. Chem. 276, 7366.
      Bhat, R.V., et al. 2000. Proc. Natl. Acad. Sci. USA 97, 11074.
      Product Information
      FormLiquid
      FormulationIn Dulbecco's PBS (without Mg2+ and Ca2+), 50% glycerol, 1 mg/ml BSA, pH 7.3.
      Negative controlNormal rabbit serum
      Positive controlSerum starved insulin-treated 3T3-L1 cells
      Preservative≤0.1% sodium azide
      Quality LevelMQ100
      Applications
      Key Applications Dot Blot
      Enzyme-Linked Immunosorbent Assay
      Immunoblotting (Western Blotting)
      Immunocytochemistry
      Application NotesDot Blot (see comments)
      ELISA (see coments)
      Immunoblotting (1:1000)
      Immunocytochemistry (1:400)
      Application CommentsTo generate a positive control, serum starve 3T3-L1 cells for 3 h, then stimulate with 100 nM insulin or 100 ng/ml IGF-1 for 5 min. This antibody has also been reported to work for ELISA and dot blots. At a 1:1000 dilution there is enough antibody for 10 immunoblots at 10 ml/blot. Antibody must be titrated for optimal results in individual systems.
      Biological Information
      Immunogena synthetic phosphopeptide corresponding to amino acids surrounding the Tyr279/216 phosphorylation sites of human GSK-3α/β
      ImmunogenHuman
      HostRabbit
      IsotypeIgG
      Species Reactivity
      • Human
      • Mouse
      • Rat
      Antibody TypePolyclonal Antibody
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Standard Handling
      Storage -20°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      ST1013-10TCN 04055977208665

      Documentation

      PhosphoDetect™ Anti-GSK-3α/β (pTyr279/216) Rabbit pAb Certificates of Analysis

      TitleLot Number
      ST1013

      References

      Reference overview
      Papasozomenos, S.C., et al. 2002. PNAS 99, 1140.
      Kirschenbaum, F., et al. 2001. J. Biol. Chem. 276, 7366.
      Bhat, R.V., et al. 2000. Proc. Natl. Acad. Sci. USA 97, 11074.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision27-May-2008 JSW
      SynonymsAnti-Glycogen Synthase Kinase-3α/β, Phospho-Specific (Tyr279/216)
      ApplicationDot Blot (see comments)
      ELISA (see coments)
      Immunoblotting (1:1000)
      Immunocytochemistry (1:400)
      Application Data
      Detection of human GSK-3β, phosphorylated at Tyr216 by immunoblotting. Samples: Whole cell lysate from control cells (lane 1) or control lysate spiked with human recombinant GSK-3β (lanes 2-5). Primary antibody: PhosphoDetect™ Anti-Glycogen Synthase Kinase-3α/β (pTyr279/216) Rabbit pAb (Cat. No. ST1013) (1:1000) pre-incubated without peptide (lanes 1, 5), or with phosphopeptide immunogen (lane 2), a generic phosphoserine-containing peptide (lane 3), or non-phosphopeptide corresponding to the immunogen (lane 4). Detection: chemiluminescence.
      DescriptionImmunoaffinity purified rabbit polyclonal antibody. Recognizes the ~51 kDa phosphorylated GSK-3α protein phosphorylated at Ser279 and the ~47 kDa GSK-3β protein phosphorylated at Ser216.
      BackgroundGSK-3α contains a phosphate on tyrosine 279, and GSK-3β contains a phosphate on tyrosine 216. GSK is a protein serine kinase involved in the control of regulatory proteins like glycogen synthase and the transcription factor c-Jun. It has also been associated with the regulation of the microtubule-binding protein Tau, and may be involved in the pathogenesis of Alzheimer's Disease.
      HostRabbit
      Immunogen speciesHuman
      Immunogena synthetic phosphopeptide corresponding to amino acids surrounding the Tyr279/216 phosphorylation sites of human GSK-3α/β
      IsotypeIgG
      Specieshuman, mouse, rat
      Positive controlSerum starved insulin-treated 3T3-L1 cells
      Negative controlNormal rabbit serum
      FormLiquid
      FormulationIn Dulbecco's PBS (without Mg2+ and Ca2+), 50% glycerol, 1 mg/ml BSA, pH 7.3.
      Preservative≤0.1% sodium azide
      CommentsTo generate a positive control, serum starve 3T3-L1 cells for 3 h, then stimulate with 100 nM insulin or 100 ng/ml IGF-1 for 5 min. This antibody has also been reported to work for ELISA and dot blots. At a 1:1000 dilution there is enough antibody for 10 immunoblots at 10 ml/blot. Antibody must be titrated for optimal results in individual systems.
      Storage Avoid freeze/thaw
      -20°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Toxicity Standard Handling
      ReferencesPapasozomenos, S.C., et al. 2002. PNAS 99, 1140.
      Kirschenbaum, F., et al. 2001. J. Biol. Chem. 276, 7366.
      Bhat, R.V., et al. 2000. Proc. Natl. Acad. Sci. USA 97, 11074.

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