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109253 Papanicolaou's solution 1a Harris' hematoxylin solution

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109253
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          1.09253.1022
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              1.09253.2500
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                  Description
                  Catalogue Number109253
                  SynonymsHematoxylin solution acc. to Harris, Colour solution acc. to Papanicolaou, Harris' Hematoxylin solution
                  DescriptionPapanicolaou’s solution 1a Harris hematoxylin solution
                  OverviewPapanicolaou’s solution 1a Harris hematoxylin solution - for cytology, is a ready-to-use solution used for human-medical cell diagnosis and serve the purpose of the cytological investigation of sample material of human origin, for example, gynecological and clinico-cytological specimen materials. Papanicolaou’s technique is the most used staining procedure for cytological specimen is and is intended for the the staining of exfoliative cells in cytological specimens. In the first step, the cell nuclei are stained either progressively or regressively with a hematoxylin solution, (Hematoxylin solution modified acc.to Gill II, (Cat.no. 105175), Papanicolaou’s solution 1a Harris hematoxylin solution, (Cat.no. 109253) or Papanicolaou’s solution 1b Hematoxylin solution S, (Cat.no. 109254)). Nuclei are stained blue to dark violet. In the progressive hematoxylin staining method, staining is carried out to the endpoint, after which the slide is blued in tapwater. With the regressive method the material is over-stained and the excess of staining solution is removed by acid rinsing steps, followed by the bluing step. The structures of nuclei are more differentiated and better visible by the regressive method. The second staining step is cytoplasmic staining by (Papanicolaou’s solution 2b Orange II solution, (Cat. no. 1106887) or Papanicolaou’s solution 2a Orange G solution (OG6), (Cat. no. 106888)), especially for demonstration of mature and keratinized cells. The target structures are stained orange in different intensities. In the third staining step is used the so-called polychrome solution, a mixture of eosin, light green SF and Bismarck brown, Papanicolaou’s solution 3a polychromatic solution EA 31, (Cat.no 109271), Papanicolaou’s solution 3b polychromatic solution EA 50, (Cat.no. 109272), Papanicolaou’s solution 3c polychromatic solution EA 65,(Cat.no. 109270), or Papanicolaou’s solution 3d polychromatic solution EA 65, (Cat.no.109269). The polychrome solution is used for demonstration of differentiation of squamous cells. Both Papanicolaou’s solution 1a Harris hematoxylin solution(Cat.no. 109253) and Papanicolaou’s solution 1b Hematoxylin solution S (Cat.no. 109254) give a blue to dark violet staining result with clinical specimen material.
                  A 500 ml bottle of either solution is sufficient for 1500 -2500 stainings. It is registered as IVD and CE certified product and can be used for clinical diagnostics. For more details, please see instructions for use (IFU). The IFU can be downloaded from this webpage.
                  References
                  Product Information
                  HS Code3212 90 00
                  Quality LevelMQ400
                  Applications
                  Applicationfor cytology
                  Biological Information
                  Physicochemical Information
                  Boiling point100 °C (1013 hPa)
                  Density1.04 g/cm3 (20 °C)
                  pH value2.5 - 3.0 (H₂O, 20 °C)
                  Dimensions
                  Materials Information
                  Toxicological Information
                  Safety Information according to GHS
                  Hazard Pictogram(s) Hazard Pictogram(s)
                  Hazard Statement(s)H318: Causes serious eye damage.
                  EUH210: Safety data sheet available on request.
                  Precautionary Statement(s)P280: Wear eye protection/ face protection.
                  P305 + P351 + P338: IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
                  Signal WordDanger
                  Storage class10 - 13 Other liquids and solids
                  WGKWGK 1 slightly hazardous to water
                  Disposal28
                  Aqueous solutions: Container D.
                  Safety Information
                  Product Usage Statements
                  Storage and Shipping Information
                  StorageStore at +15°C to +25°C.
                  Packaging Information
                  Transport Information
                  Supplemental Information
                  Specifications
                  Suitability for microscopy (Vaginal smear)passes test
                  Nucleiblue to dark violet
                  Cyanophilic Cytoplasm (basophilic)blue-green
                  Eosinophilic Cytoplasm (acidophilic)pink
                  Global Trade Item Number
                  Catalogue Number GTIN
                  1.09253.0500 04022536112466
                  1.09253.1000 04022536676661
                  1.09253.1022 04022536707433
                  1.09253.2500 04022536112480

                  Documentation

                  Papanicolaou's solution 1a Harris' hematoxylin solution SDS

                  Title

                  Safety Data Sheet (SDS) 

                  Papanicolaou's solution 1a Harris' hematoxylin solution Certificates of Analysis

                  Product Number PackagingSpecificationLot Number
                  1.09253.0500 Plastic bottlePDF Specification Document
                  1.09253.1000 Plastic bottlePDF Specification Document
                  1.09253.1022 Plastic bottlePDF Specification Document
                  1.09253.2500 Plastic bottlePDF Specification Document

                  User Guides

                  Title
                  User Guide (IFU) - 109253

                  Related Products & Applications

                  Accessories (Primary)

                  Catalogue Number Description  
                  106887 Papanicolaou’s solution 2b Orange II solution Show Pricing & Availability
                  106888 Papanicolaou's solution 2a Orange G solution (OG 6) Show Pricing & Availability
                  109254 Papanicolaou's solution 1b Hematoxylin solution S Show Pricing & Availability
                  109269 Papanicolaou's solution 3d polychromatic solution EA 65 Show Pricing & Availability
                  109271 Papanicolaou's solution 3a polychromatic solution EA 31 Show Pricing & Availability
                  109272 Papanicolaou's solution 3b polychromatic solution EA 50 Show Pricing & Availability
                  115938 Hematoxylin monohydrate (C.I.75290) Show Pricing & Availability

                  Accessories (Secondary)

                  Catalogue Number Description  
                  104302 Hematoxylin cryst. (C.I. 75290) Show Pricing & Availability
                  105175 Hematoxylin solution modified acc. to Gill II Show Pricing & Availability
                  106887 Papanicolaou’s solution 2b Orange II solution Show Pricing & Availability
                  106888 Papanicolaou's solution 2a Orange G solution (OG 6) Show Pricing & Availability
                  109254 Papanicolaou's solution 1b Hematoxylin solution S Show Pricing & Availability
                  109269 Papanicolaou's solution 3d polychromatic solution EA 65 Show Pricing & Availability
                  109271 Papanicolaou's solution 3a polychromatic solution EA 31 Show Pricing & Availability
                  109272 Papanicolaou's solution 3b polychromatic solution EA 50 Show Pricing & Availability

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                  Categories

                  IVD/OEM Materials & Reagents > Microscopy > Cytology > Cytological Staining Reagents