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53702 PRONASE® Protease, Streptomyces griseus

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53702
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Overview

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53702-10KUCN
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      53702-250KUCN
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          53702-25KUCN
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              Glass bottle 25 ku
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              53702-500KUCN
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                  Glass bottle 500 ku
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                  53702-50KUCN
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                      Description
                      OverviewA non-specific protease liquefies mucins and digests proteins to free amino acids. Can be used to isolate living chondrocytes. Most active at neutral pH, but stable over wide range of pH and temperature. Note: 1KU = 1000 units.
                      Note: 1 KU = 1000 units.
                      Catalogue Number53702
                      Brand Family Calbiochem®
                      References
                      ReferencesNarahashi, Y., et al. 1968. J. Biochem. 64, 427.
                      Product Information
                      CAS number9036-06-0
                      Activity≥45,000 proteolytic units/g dry weight
                      Unit of DefinitionOne unit is defined as the amount of enzyme that will liberate a digestion product equivalent to 25 µg of tyrosine per min at 40°C, pH 7.5.
                      FormLyophilized
                      FormulationLyophilized from a solution containing calcium acetate as a stabilizer.
                      Hygroscopic Hygroscopic
                      Quality LevelMQ100
                      Applications
                      Biological Information
                      Physicochemical Information
                      Dimensions
                      Materials Information
                      Toxicological Information
                      Safety Information according to GHS
                      RTECSUK9595000
                      Safety Information
                      R PhraseR: 20-36/37/38-42/43

                      Harmful by inhalation.
                      Irritating to eyes, respiratory system and skin.
                      May cause sensitization by inhalation and skin contact.
                      S PhraseS: 22-26-36

                      Do not breathe dust.
                      In case of contact with eyes, rinse immediately with plenty of water and seek medical advice.
                      Wear suitable protective clothing.
                      Product Usage Statements
                      Storage and Shipping Information
                      Ship Code Ambient Temperature Only
                      Toxicity Harmful
                      Storage +2°C to +8°C
                      Hygroscopic Hygroscopic
                      Do not freeze Ok to freeze
                      Special InstructionsFollowing reconstitution, store in the refrigerator (4°C). Stock solutions are stable for 24 h at 4°C.
                      Packaging Information
                      Transport Information
                      Supplemental Information
                      Specifications
                      Global Trade Item Number
                      Catalogue Number GTIN
                      53702-10KUCN 04055977195835
                      53702-250KUCN 04055977195859
                      53702-25KUCN 04055977195842
                      53702-500KUCN 07790788051259
                      53702-50KUCN 04055977195866

                      Documentation

                      PRONASE® Protease, Streptomyces griseus SDS

                      Title

                      Safety Data Sheet (SDS) 

                      PRONASE® Protease, Streptomyces griseus Certificates of Analysis

                      TitleLot Number
                      53702

                      References

                      Reference overview
                      Narahashi, Y., et al. 1968. J. Biochem. 64, 427.

                      Brochure

                      Title
                      Bulk Product Guide
                      Data Sheet

                      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

                      Revision17-September-2007 RFH
                      DescriptionA non-specific protease liquefies mucins and digests proteins to free amino acids. Can be used to isolate living chondrocytes. Most active at neutral pH, but it is stable over a wide pH and temperature range. Has an optimal pH of 7.5. Note: 1 KU = 1000 units.
                      FormLyophilized
                      FormulationLyophilized from a solution containing calcium acetate as a stabilizer.
                      CAS number9036-06-0
                      RTECSUK9595000
                      Activity≥45,000 proteolytic units/g dry weight
                      Unit definitionOne unit is defined as the amount of enzyme that will liberate a digestion product equivalent to 25 µg of tyrosine per min at 40°C, pH 7.5.
                      SolubilityH₂O (10 mg/ml)
                      Storage +2°C to +8°C
                      Hygroscopic
                      Do Not Freeze Ok to freeze
                      Special InstructionsFollowing reconstitution, store in the refrigerator (4°C). Stock solutions are stable for 24 h at 4°C.
                      Toxicity Harmful
                      ReferencesNarahashi, Y., et al. 1968. J. Biochem. 64, 427.