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499260 Osteopontin, His•Tag®, Mouse, Recombinant, Mouse

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: rmOPN

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499260
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Overview

Replacement Information

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499260-50UGCN
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      		 Plastic ampoule 50 μg
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      Description
      OverviewRecombinant, mouse osteopontin fused at the C-terminus to a His•Tag® sequence and expressed in NSO-derived Leu17-Asn294 (Glu99Gly) murine myeloma cells. A secreted, highly acidic, Ca2+-binding, RGD-containing, phosphorylated glycoprotein. Chemotactic for macrophages, smooth muscle cells, endothelial cells, and glial cells. Has been shown to inhibit nitric oxide production and cytotoxicity by activated macrophages. Plays a significant role in inducing granulomas of various origin. OPN is also involved in the migration of embryonic fibroblasts, macrophages, and metastatic cells.
      M.W. 65,000 and 30,000 under reducing conditions.
      Catalogue Number499260
      Brand Family Calbiochem®
      SynonymsrmOPN
      References
      ReferencesXie, Y., et al. 2001. Kidney Int. 60, 1645.
      Chiba, S., et al. 2000. Microbiol. Immunol. 44, 319.
      Miyazaki, Y., et al. 1990. J. Biol. Chem. 265, 14432.
      Product Information
      FormLyophilized
      FormulationLyophilized from 0.2 µm sterile-filtered PBS, 50 µg BSA/µg rmOPN.
      Quality LevelMQ100
      Applications
      Biological Information
      Biological activityED50 = 0.25-1.5 µg/ml
      based on the addition of 1 x 10⊃5 cells per well coated with osteopontin and incubated for 1 hr at 37°C.
      Purity≥95% by SDS-PAGE
      Physicochemical Information
      ContaminantsEndotoxins: ≤100 pg/µg rmOPN
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Ambient Temperature Only
      Toxicity Standard Handling
      Storage ≤ -70°C
      Do not freeze Ok to freeze
      Special InstructionsFollowing reconstitution, aliquot and freeze (-70°C). Stock solutions are stable for up to 1 month at 4°C and for up to 3 months at -70°C. Avoid freeze/thaw cycles of stock solutions.
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      499260-50UGCN 04055977199918

      Documentation

      Osteopontin, His•Tag®, Mouse, Recombinant, Mouse SDS

      Title

      Safety Data Sheet (SDS) 

      Osteopontin, His•Tag®, Mouse, Recombinant, Mouse Certificates of Analysis

      TitleLot Number
      499260

      References

      Reference overview
      Xie, Y., et al. 2001. Kidney Int. 60, 1645.
      Chiba, S., et al. 2000. Microbiol. Immunol. 44, 319.
      Miyazaki, Y., et al. 1990. J. Biol. Chem. 265, 14432.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision14-June-2019 JSW
      SynonymsrmOPN
      DescriptionRecombinant, mouse osteopontin fused at the C-terminus to a His•Tag® sequence and expressed in NSO-derived Leu17-Asn294 (Glu99Gly) murine myeloma cells. A secreted, highly acidic, calcium-binding, RGD-containing, phosphorylated glycoprotein. Osteopontin is expressed in several cell types including macrophages, fibroblasts, epithelial cells, T cells and osteoclasts in response to activation by cytokines, growth factors or inflammatory mediators. Mouse OPN cDNA encodes a 294 amino acid protein that has a 16 amino acid predicted signal peptide, which is cleaved to generate 278 amino acid mature protein. The mature protein contains an integrin binding arginine-glycine-aspartic acid (RGD) sequence. Exhibits some renoprotective actions in renal injury, such as increasing tolerance to acute ischemia, inhibiting inducible nitric oxide synthase, and reducing cell peroxide levels.
      FormLyophilized
      FormulationLyophilized from 0.2 µm sterile-filtered PBS, 50 µg BSA/µg rmOPN.
      Purity≥95% by SDS-PAGE
      ContaminantsEndotoxins: ≤100 pg/µg rmOPN
      Biological activityED50 = 0.25-1.5 µg/ml
      based on the addition of 1 x 10⊃5 cells per well coated with osteopontin and incubated for 1 hr at 37°C.
      SolubilityReconstitute to ≥100 µg/ml with sterile PBS.
      Storage ≤ -70°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing reconstitution, aliquot and freeze (-70°C). Stock solutions are stable for up to 1 month at 4°C and for up to 3 months at -70°C. Avoid freeze/thaw cycles of stock solutions.
      Toxicity Standard Handling
      ReferencesXie, Y., et al. 2001. Kidney Int. 60, 1645.
      Chiba, S., et al. 2000. Microbiol. Immunol. 44, 319.
      Miyazaki, Y., et al. 1990. J. Biol. Chem. 265, 14432.