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CBA001 InnoZyme™ Cathepsin B Activity Assay Kit, Fluorogenic

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CBA001
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Overview

Key Spec Table

Detection Methods
Fluorometric

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      Description
      OverviewA sensitive fluorogenic assay for the measurement of cathepsin B activity (Excitation max: ~360-380 nm; Emission max: ~430-460 nm). Cathepsin B is a cysteine proteinase which has been associated with increased invasivessness and development of the malignant cell phenotype. Cathepsin B has also been implicated in inflammatory airway disease.
      Catalogue NumberCBA001
      Brand Family Calbiochem®
      Application Data
      Calibration curve generated by various dilutions of the free AMC standard included in the kit (above).
      Materials Required but Not Delivered• Pipettors or multi-channel pipettors calibrated to target volume
      • 37°C incubator
      • Microplate reader capable of measuring fluorescence at wavelengths 360-380 nm for excitation and 440-460 nm for emission
      • Ice bucket
      References
      ReferencesBervar, A., et al. 2003. Biol. Chem. 384, 447.
      Grigolo, B. et al. 2003. Biomaterials 24, 1751.
      Scolaris, A., et al. 2002. Biol. Chem. 383, 1297.
      Murata, M., et al. 1991. FEBS Lett. 280, 307.
      Berquin, I.M. and Sloane, B.F. 1996. Adv. Exp. Med. Biol. 389, 281.
      Burnett, D., 1995. Arch. Biochem. Biophys. 317, 305.
      Reddy, V.Y., et al. 1995. Proc. Natl. Acad. Sci. 92, 3849.
      Buttle, D.J., 1994. In: Immunopharmacology of Joints and Connective Tissue (Dingle, J.T. and Davies, M.E., eds) London: Academic Press. 225.
      Barrett, A.J. and Kirschke, H., 1981. Methods Enzymol. 80, 535.
      Product Information
      Detection methodFluorometric
      Form96 Tests
      Format96-well plate
      Kit containsCathepsin B Enzyme, Calibration Standard, Cathepsin B Substrate, Assay Buffer, Cathepsin B Inhibitor, Reduction Reagent, Cell Lysis Buffer, Microtiter Plate, Plate Sealer, and a user protocol.
      Positive controlCathepsin B
      Quality LevelMQ100
      Biological Information
      Assay range0.09 - 100 ng/ml
      Assay time30 min
      Sample TypeCells and tissues
      Physicochemical Information
      Sensitivity0.63 ng/ml
      Product Usage Statements
      Intended useThe Calbiochem® Cathepsin B Activity Assay Kit, Fluorogenic is designed to measure cathepsin B activity in tissues extracts and cell lysates, and for screening cathepsin B inhibitors.

      Note: Following initial use the control Cathepsin B should be dispensed into aliquots and stored at -20°C. Avoid freeze/thaw cycles
      Storage and Shipping Information
      Ship Code Dry Ice Only
      Toxicity Multiple Toxicity Values, refer to MSDS
      Storage Multiple storage requirements
      Storage ConditionsStore the unopened components at -20°C and -70°C as indicated on the labels. All kit components, once opened, can be stored under the following conditions:

      Assay Buffer, Cell Lysis Buffer, 96-Well Plate, and Plate Sealers at Room Temperature

      Cathepsin B Inhibitor, Reduction Reagent, Cathepsin B Substrate, and Calibration Standard at -20°C

      Control Cathepsin B(aliquot) at -70°C
      Protect from Light Protect from light
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Supplemental Information
      Kit containsCathepsin B Enzyme, Calibration Standard, Cathepsin B Substrate, Assay Buffer, Cathepsin B Inhibitor, Reduction Reagent, Cell Lysis Buffer, Microtiter Plate, Plate Sealer, and a user protocol.
      Global Trade Item Number
      Catalogue Number GTIN
      CBA001-1KITCN 04055977220810