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374805 Sigma-AldrichHemocyanin, Keyhole Limpet, Megathura crenulata

Hemocyanin, Keyhole Limpet, Megathura crenulata, CAS 9013-72-3, is a large, multi-subunit, oxygen-carrying, metalloprotein that is used as a carrier protein in the production of antibodies.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

SDS
CoA
References
Data Sheet

Synonyms: KLH

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Overview

Replacement Information

Pricing & Availability

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Description
Overview	Stable solid containing more than 60% protein. Protein is 90% undissociated hemocyanin.
Overview	Protein:≥60%; copper to protein ratio: ≥1.5 x 10^-3
Catalogue Number	374805
Brand Family	Calbiochem®
Synonyms	KLH

References
References	Hayashi, K., et al. 1992. Virchows Archiv. B Cell Pathol. 63, 37. Shuler, K.R., et al. 1992. J. Immunol. Methods 156, 137. Erlanger, B.F. 1980. Methods Enzymol. 70, 85.

Product Information
CAS number	9013-72-3
Form	Blue to blue-gray lyophilized solid
Formulation	Lyophilized from BES buffer, MgSO₄.
Preservative	None
Quality Level	MQ200

Applications
Application	Hemocyanin, Keyhole Limpet, Megathura crenulata, CAS 9013-72-3, is a large, multi-subunit, oxygen-carrying, metalloprotein that is used as a carrier protein in the production of antibodies.
Application Comments	Protein concentration: ≥60% Copper: ≥0.09% Copper to Protein ratio: ≥1.5 x 10^-3

Biological Information
Purity	≥90% by size exclusion chromatography

Physicochemical Information

Dimensions

Materials Information

Toxicological Information

Safety Information according to GHS

Safety Information

Product Usage Statements

Storage and Shipping Information
Ship Code	Ambient Temperature Only
Toxicity	Standard Handling
Storage	+2°C to +8°C
Do not freeze	Ok to freeze
Special Instructions	Dissolve at 5-10 mg/ml (protein) in PBS or other physiological buffer by GENTLE addition of the buffer (e.g. slowly added down the side of the tube). DO NOT vortex or agitate vigorously to dissolve product. (KLH is an extremely large protein complex and has a tendency to aggregate and precipitate from solutions.) For further dilutions, use an appropriate buffer and maintain the magnesium concentration at ≥10 mM. Solutions should be stored in the refrigerator (4°C).

Packaging Information

Transport Information

Supplemental Information

Specifications

Global Trade Item Number
Catalogue Number	GTIN
374805-1GMCN	04055977191226
374805-250MGCN	07790788059316

Documentation

Hemocyanin, Keyhole Limpet, Megathura crenulata SDS

Title
Safety Data Sheet (SDS)

Hemocyanin, Keyhole Limpet, Megathura crenulata Certificates of Analysis

Title	Lot Number
374805	Enter Lot Number

References

Reference overview
Hayashi, K., et al. 1992. Virchows Archiv. B Cell Pathol. 63, 37. Shuler, K.R., et al. 1992. J. Immunol. Methods 156, 137. Erlanger, B.F. 1980. Methods Enzymol. 70, 85.

Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision	10-March-2011 JSW
Synonyms	KLH
Description	Hemocyanin purified from Keyhole Limpet (Mega thura crenulata). Protein:≥60%; copper to protein ratio: ≥1.5 x 10^-3
Background	Keyhole limpet hemocyanin (KLH), a carrier protein with a molecular weight ranging from 3,000,000-7,500,000, has many available primary amines to facilitate protein conjugation. KLH is used to immunize animals to elicit antibodies to small molecules (haptens) by covalent conjugation.
Form	Blue to blue-gray lyophilized solid
Formulation	Lyophilized from BES buffer, MgSO₄.
CAS number	9013-72-3
Purity	≥90% by size exclusion chromatography
Preservative	None
Comments	Protein concentration: ≥60% Copper: ≥0.09% Copper to Protein ratio: ≥1.5 x 10^-3
Storage	+2°C to +8°C
Do Not Freeze	Ok to freeze
Special Instructions	Dissolve at 5-10 mg/ml (protein) in PBS or other physiological buffer by GENTLE addition of the buffer (e.g. slowly added down the side of the tube). DO NOT vortex or agitate vigorously to dissolve product. (KLH is an extremely large protein complex and has a tendency to aggregate and precipitate from solutions.) For further dilutions, use an appropriate buffer and maintain the magnesium concentration at ≥10 mM. Solutions should be stored in the refrigerator (4°C).
Toxicity	Standard Handling
Merck USA index	14, 4647
References	Hayashi, K., et al. 1992. Virchows Archiv. B Cell Pathol. 63, 37. Shuler, K.R., et al. 1992. J. Immunol. Methods 156, 137. Erlanger, B.F. 1980. Methods Enzymol. 70, 85.

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