Millipore Sigma Vibrant Logo

109218 Gram's crystal violet solution

for the Gram staining method

Gram's crystal violet solution MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
109218
View Pricing & Availability

Overview

Replacement Information

Pricing & Availability

Catalogue Number AvailabilityPackaging Qty/Pack Price Quantity
1.09218.0500
Retrieving availability...
—
Limited Availability Limited Availability
In Stock 
Discontinued
Limited Quantities Available
Availability to be confirmed
    Remaining : Will advise
      Remaining : Will advise
      Will advise
      Contact Customer Service
      Contact Customer Service

      		 Plastic bottle 500 ml
      Retrieving price...
      Price could not be retrieved
      Minimum Quantity is a multiple of
      Maximum Quantity is
      Upon Order Completion More Information
      You Saved ()
       
      —
      Request Pricing
      		 —
      1.09218.1022
      Retrieving availability...
      —
      Limited Availability Limited Availability
      In Stock 
      Discontinued
      Limited Quantities Available
      Availability to be confirmed
        Remaining : Will advise
          Remaining : Will advise
          Will advise
          Contact Customer Service
          Contact Customer Service

          		 Plastic bottle 20 PC
          Retrieving price...
          Price could not be retrieved
          Minimum Quantity is a multiple of
          Maximum Quantity is
          Upon Order Completion More Information
          You Saved ()
           
          —
          Request Pricing
          		 —
          1.09218.2500
          Retrieving availability...
          —
          Limited Availability Limited Availability
          In Stock 
          Discontinued
          Limited Quantities Available
          Availability to be confirmed
            Remaining : Will advise
              Remaining : Will advise
              Will advise
              Contact Customer Service
              Contact Customer Service

              		 Plastic bottle 2.5 l
              Retrieving price...
              Price could not be retrieved
              Minimum Quantity is a multiple of
              Maximum Quantity is
              Upon Order Completion More Information
              You Saved ()
               
              —
              Request Pricing
              		 —
              Description
              Catalogue Number109218
              DescriptionGram's crystal violet solution
              OverviewGram's crystal violet solution for the Gram staining method, is a ready-to-use solution used in bacteriology. The Gram staining allows a fast differentiation of bacteria in Gram-positive and Gram-negative.The mureine structure of the bacteria wall is the basis of the color affinity. In the first step, bacteria will be stained with Gram's crystal violet solution (Cat. No.109218), an aniline dye. After the treatment with iodine solution (Lugol’s solution stabilized with PVP, Cat No.100567), a dye-iodine complex will form. During the decolorizing step (Gram’s decolorizing solution, Cat. No. 110218), this complex stays in the multilayer mureine structure of the cell wall of Gram-positive bacteria - they will appear blue-violet. Gram-negative bacteria, by contrast, have a cell wall consisting of a single-layered murein structure, and correspondingly re-release the staining dye with the decoloring solution. Gram-negative bacteria will be counterstained with safranine solution (Gram's safranine solution (Cat.no. 109217) and will then appear pink to red.
              A bottle with 500 ml Gram's crystal violet solution is sufficient for staining up to 250 slides. This product is registered as IVD and CE marked. For more details, please see instructions for use (IFU). The IFU can be downloaded from this webpage.
              References
              Product Information
              HS Code3212 90 90
              Quality LevelMQ400
              Applications
              Applicationfor the Gram staining method
              Biological Information
              Physicochemical Information
              Density0.99 g/cm3 (20 °C)
              Flash point47 °C
              Dimensions
              Materials Information
              Toxicological Information
              Safety Information according to GHS
              Hazard Pictogram(s) Hazard Pictogram(s)
              Hazard Statement(s)H226: Flammable liquid and vapour.
              H412: Harmful to aquatic life with long lasting effects.
              Precautionary Statement(s)P210: Keep away from heat, hot surfaces, sparks, open flames and other ignition sources. No smoking.
              P233: Keep container tightly closed.
              P240: Ground and bond container and receiving equipment.
              P241: Use explosion-proof electrical/ ventilating/ lighting equipment.
              P242: Use non-sparking tools.
              P273: Avoid release to the environment.
              Signal WordWarning
              Storage class3 Flammable liquids
              WGKWGK 2 obviously hazardous to water
              Disposal28
              Aqueous solutions: Container D.
              Safety Information
              Categories of dangerflammable, dangerous for the environment
              Product Usage Statements
              Storage and Shipping Information
              StorageStore at +15°C to +25°C.
              Packaging Information
              Transport Information
              Declaration (railroad and road) ADR, RIDKein Gefahrgut
              Declaration (transport by air) IATA-DGRNo Dangerous Good
              Declaration (transport by sea) IMDG-CodeNo Dangerous Good
              Supplemental Information
              Specifications
              Suitability for microscopypasses test
              gram-positve microorganismsblue-violet
              gram-negative microorganismspink to red
              Global Trade Item Number
              Catalogue Number GTIN
              1.09218.0500 04022536112077
              1.09218.1022 04022536707419
              1.09218.2500 04022536557403

              Related Products & Applications

              Alternative Products

              Catalogue Number Description  
              111885 Gram-Color Show Pricing & Availability

              Related Products

              Catalogue Number Description  
              111885 Gram-Color Show Pricing & Availability

              Accessories (Primary)

              Catalogue Number Description  
              100567 Lugol's solution Show Pricing & Availability
              109217 Gram's safranine solution Show Pricing & Availability
              109261 Lugol's solution (diluted iodine-potassium iodide solution) Show Pricing & Availability
              110218 Gram's decolorizing solution Show Pricing & Availability

              Accessories (Secondary)

              Catalogue Number Description  
              100567 Lugol's solution Show Pricing & Availability
              109217 Gram's safranine solution Show Pricing & Availability
              109261 Lugol's solution (diluted iodine-potassium iodide solution) Show Pricing & Availability
              111885 Gram-Color Show Pricing & Availability

              Related Products By: Application Facete

              Gram bacteria Detection
              Bacteria Staining

              Categories

              IVD/OEM Materials & Reagents > Microscopy > Bacteriology > Gram-Bacteria Staining