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109204 Giemsa's azur eosin methylene blue solution

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109204
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              1.09204.1022
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                  1.09204.2500
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                      1.09204.9025
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                          Barrel PE/met 25 l
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                          Description
                          Catalogue Number109204
                          DescriptionGiemsa's azur-eosin-methylene blue solution
                          OverviewGiemsa’s Azure Eosin Methylene Blue solution - for microscopy, can be used for the staining of blood and bone marrow smears, paraffin sections and clinical-cytological specimens. Giemsa staining is one of the standard procedures in histology, demonstrating various cells with their morphological features better than with H&E stain. When used in hematological applications, Giemsa’s stain is frequently used in combination with other staining solutions, e. g. with May-Grünwald’s solution for Pappenheim (MGG) overview staining. This ready-to-use staining solution generally stains the nuclei red, based on the molecular interaction between the eosin Y dye and an Azure B-DNA complex. Both dyes assemble to an Eosin Y - Azure B-DNA complex and the intensity of the resulting stain depends on the content of Azure B and the ratio of Azure B : Eosin Y. Furthermore, the resulting stain can vary depending on the influence of fixation, staining times, pH-value of the solutions or buffer substances. In histology and clinico-cytological applications, Giemsa’s staining without additional stainings is used as an extended overview staining method. In this method, the color of the various cell components is influenced by pretreatment of the specimen material. Here, chromatin-containing structures (e. g. cell nuclei) appear in various blue shades, while the acidophilic components show up in a variety of red shades. The 500 ml bottle is sufficient for 3500-5000 stainings. This product is registered as IVD and CE marked. For more details, please see instructions for use (IFU). The IFU can be downloaded from this webpage.
                          References
                          Product Information
                          HS Code3212 90 90
                          Quality LevelMQ400
                          Applications
                          Applicationfor microscopy
                          Biological Information
                          Physicochemical Information
                          Boiling point>65 °C (1013 hPa)
                          Density0.99 g/cm3 (20 °C)
                          Explosion limit5.5 - 44 %(V) Methanol
                          Flash point18 °C
                          Ignition temperature455 °C
                          pH value6.0 - 8.2 (H₂O, 20 °C) (undiluted)
                          Dimensions
                          Materials Information
                          Toxicological Information
                          Safety Information according to GHS
                          Hazard Pictogram(s) Hazard Pictogram(s)
                          Hazard Pictogram(s)
                          Hazard Pictogram(s)
                          Hazard Statement(s)H225: Highly flammable liquid and vapour.
                          H301 + H311 + H331: Toxic if swallowed, in contact with skin or if inhaled.
                          H317: May cause an allergic skin reaction.
                          H370: Causes damage to organs.
                          Precautionary Statement(s)P210: Keep away from heat, hot surfaces, sparks, open flames and other ignition sources. No smoking.
                          P233: Keep container tightly closed.
                          P280: Wear protective gloves/ protective clothing/ eye protection/ face protection/ hearing protection.
                          P301 + P310: IF SWALLOWED: Immediately call a POISON CENTER/doctor.
                          P303 + P361 + P353: IF ON SKIN (or hair): Take off immediately all contaminated clothing. Rinse skin with water.
                          P304 + P340 + P311: IF INHALED: Remove person to fresh air and keep comfortable for breathing. Call a POISON CENTER/doctor.
                          P403 + P233: Store in a well-ventilated place. Keep container tightly closed.
                          Signal WordDanger
                          Storage class3 Flammable liquids
                          WGKWGK 2 obviously hazardous to water
                          Disposal9
                          Carcinogenic compounds and flammable compounds labelled "Highly toxic" or "Toxic": container F. Alkyl sulfates are carcinogenic; Take particular care to avoid inhalation and skin contact. To neutralize alkyl sulfates, add dropwise (from a dropping funnel) to concentrated ice-cool Ammonia solution (Cat.No. 105426) with vigorous stirring. Before placing in container D, check the pH with pH Universal indicator strips (Cat.No. 109535).
                          Safety Information
                          Categories of dangerhighly flammable, toxic
                          Product Usage Statements
                          Storage and Shipping Information
                          StorageStore at +15°C to +25°C.
                          Packaging Information
                          Transport Information
                          Declaration (railroad and road) ADR, RIDUN 1230 , 3 (6.1), II
                          Declaration (transport by air) IATA-DGRUN 1230 , 3 (6.1), II
                          Declaration (transport by sea) IMDG-CodeUN 1230 , 3 (6.1), II
                          Supplemental Information
                          Specifications
                          Suitability for microscopypasses test
                          Erythrocytesreddish-brownish
                          Nuclei / Chromatinred to violet
                          Eosinophilic granulesreddish to red-brown
                          Neutrophilic granuleslight violet
                          Lymphocyte cytoplasmnblue
                          Monocyte cytoplasmgrey-blue
                          Thrombocytesviolet
                          Basophilic granulesdark violet
                          Nuclei (Tissue section)blue to dark blue
                          Cellsblue to dark blue
                          Collagenpale blue
                          Osteoidpale blue
                          Eosinophilic granula (Tissue section)red
                          Acidic mucopolysaccharidesreddish-violet
                          Mast cell granulesreddish-violet
                          Cartilage matrixreddish-violet
                          Acidophilic materialsorange-red
                          Helicobacter pylori
                          blue to dark blue
                          blue to dark blue
                          Global Trade Item Number
                          Catalogue Number GTIN
                          1.09204.0100 04022536111841
                          1.09204.0500 04022536111889
                          1.09204.1000 04022536720081
                          1.09204.1022 04022536707389
                          1.09204.2500 04022536111926
                          1.09204.9025 04022536111940

                          Documentation

                          Giemsa's azur eosin methylene blue solution SDS

                          Title

                          Safety Data Sheet (SDS) 

                          Giemsa's azur eosin methylene blue solution Certificates of Analysis

                          Product Number PackagingSpecificationLot Number
                          1.09204.0100 Glass bottlePDF Specification Document
                          1.09204.0500 Plastic bottlePDF Specification Document
                          1.09204.1000 Plastic bottlePDF Specification Document
                          1.09204.1022 Plastic bottlePDF Specification Document
                          1.09204.2500 Plastic bottlePDF Specification Document
                          1.09204.9025 Barrel PE/metPDF Specification Document

                          User Guides

                          Title
                          User Guide (IFU) - 109204

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                          Categories

                          IVD/OEM Materials & Reagents > Microscopy > Hematology > Rapid Stainings, Hemacolor®
                          IVD/OEM Materials & Reagents > Microscopy > Histology > Histological Staining Solutions
                          IVD/OEM Materials & Reagents > Microscopy > Cytology > Cytological Staining Reagents
                          IVD/OEM Materials & Reagents > Microscopy > Hematology > Hematological Staining Solutions
                          IVD/OEM Materials & Reagents > Microscopy > Bacteriology > General Bacteriological Staining Solutions